tetano
Editor, Senior Moderator
Zhonghua Liu Xing Bing Xue Za Zhi. 2011 Apr;32(4):376-381.
[Genetic variation of the hemagglutinin and neuraminidase of influenza B viruses isolated in Zhejiang province during 1999 - 2010.]
[Article in Chinese]
Mao HY, Zhou M, Zhang YJ, Chen Y, Xu CP, Li Z, Yan JY, Lu YY.
Source
Virus Institute, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
Abstract
OBJECTIVE:
To Characterize the genetic diversity of hemagglutinin (HA) and neuraminidase (NA) of influenza B viruses isolated in Zhejiang province during 1999 - 2010.
METHODS:
Respiratory specimens were collected from patients with flu-like syndrome during the influenza outbreaks or from the hospitals which carrying out influenza surveillance project in Zhejiang province. Samples were detected by real-time RT-PCR and isolated for influenza virus. HA(1) and NA genes of influenza B virus isolates were amplified and sequenced. Phylogenetic comparison and genetic diversity analysis were performed using the bioinformation software.
RESULTS:
A total of 34 influenza B viruses were evolved in this study including Victoria-like and Yamagata-like strains according to the results of the HI test. The mutation rate of Victoria-like HA(1) gene was 4.5% and Yamagata-like HA(1) gene was 3.4%, respectively. The Victoria-like influenza B isolates had appeared to be all re-assortants having a Victoria lineage HA and Yamagata lineage NA since 2004. The predominant type of influenza virus isolates in 2010 was also influenza B virus after the H1N1 flu pandemic in Zhejiang province. The isolated strains were antigenically and genetically similar to B/Brisbane/60/2008-the vaccine strain proposed for 2009 - 2010. Many differences of HA(1) and NA amino acids existed in the current isolates when compared to previous influenza B strains.
CONCLUSION:
Significant diversity was generated among influenza B virus isolated from 1999 to 2010 in Zhejiang province. Genetic re-assortment and antigenic drift seemed the main evolutionary mechanism on influenza B virus.
PMID:
21569670
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21569670
[Genetic variation of the hemagglutinin and neuraminidase of influenza B viruses isolated in Zhejiang province during 1999 - 2010.]
[Article in Chinese]
Mao HY, Zhou M, Zhang YJ, Chen Y, Xu CP, Li Z, Yan JY, Lu YY.
Source
Virus Institute, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
Abstract
OBJECTIVE:
To Characterize the genetic diversity of hemagglutinin (HA) and neuraminidase (NA) of influenza B viruses isolated in Zhejiang province during 1999 - 2010.
METHODS:
Respiratory specimens were collected from patients with flu-like syndrome during the influenza outbreaks or from the hospitals which carrying out influenza surveillance project in Zhejiang province. Samples were detected by real-time RT-PCR and isolated for influenza virus. HA(1) and NA genes of influenza B virus isolates were amplified and sequenced. Phylogenetic comparison and genetic diversity analysis were performed using the bioinformation software.
RESULTS:
A total of 34 influenza B viruses were evolved in this study including Victoria-like and Yamagata-like strains according to the results of the HI test. The mutation rate of Victoria-like HA(1) gene was 4.5% and Yamagata-like HA(1) gene was 3.4%, respectively. The Victoria-like influenza B isolates had appeared to be all re-assortants having a Victoria lineage HA and Yamagata lineage NA since 2004. The predominant type of influenza virus isolates in 2010 was also influenza B virus after the H1N1 flu pandemic in Zhejiang province. The isolated strains were antigenically and genetically similar to B/Brisbane/60/2008-the vaccine strain proposed for 2009 - 2010. Many differences of HA(1) and NA amino acids existed in the current isolates when compared to previous influenza B strains.
CONCLUSION:
Significant diversity was generated among influenza B virus isolated from 1999 to 2010 in Zhejiang province. Genetic re-assortment and antigenic drift seemed the main evolutionary mechanism on influenza B virus.
PMID:
21569670
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21569670