Mary Wilson
Well-known member
Posted July 25, 2022
doi: https://doi.org/10.1101/2022.07.23.501239
Terry C. Jones, Julia Schneider, Barbara Muehlemann, Talitha Veith, Joern Beheim-Schwarzbach, Julia Tesch, Marie Schmidt, Felix Walper, Tobias Bleicker, Caroline Isner, Frieder Pfaefflin, Ricardo Werner, Victor M. Corman, Christian Drosten
Abstract
Genome sequences from 47 monkeypox virus infections detected in a German university virology laboratory were analyzed in context of other sequences from the 2022 outbreak and earlier monkeypox genomes. Identical non-synonymous amino acid changes in six genes and the signature of APOBEC editing match other sequences from the European outbreak. Non-synonymous changes that were present in one to three sequences were found in 34 other genes. In sequences from two lesions of one patient, an 856 nucleotide translocation between genome termini resulted in the duplication of an initial (5-prime end) gene, and the disruption or complete deletion of four genes near the (3-prime) genome end. Orthopoxvirus genome rearrangements of this nature are known to confer fitness advantages in the face of selection pressure. This change may therefore represent an early virus adaptation in the novel widespread and sustained human-to-human context of the current monkeypox outbreak.
https://www.biorxiv.org/content/10.1101/2022.07.23.501239v1.full.pdf
doi: https://doi.org/10.1101/2022.07.23.501239
Terry C. Jones, Julia Schneider, Barbara Muehlemann, Talitha Veith, Joern Beheim-Schwarzbach, Julia Tesch, Marie Schmidt, Felix Walper, Tobias Bleicker, Caroline Isner, Frieder Pfaefflin, Ricardo Werner, Victor M. Corman, Christian Drosten
Abstract
Genome sequences from 47 monkeypox virus infections detected in a German university virology laboratory were analyzed in context of other sequences from the 2022 outbreak and earlier monkeypox genomes. Identical non-synonymous amino acid changes in six genes and the signature of APOBEC editing match other sequences from the European outbreak. Non-synonymous changes that were present in one to three sequences were found in 34 other genes. In sequences from two lesions of one patient, an 856 nucleotide translocation between genome termini resulted in the duplication of an initial (5-prime end) gene, and the disruption or complete deletion of four genes near the (3-prime) genome end. Orthopoxvirus genome rearrangements of this nature are known to confer fitness advantages in the face of selection pressure. This change may therefore represent an early virus adaptation in the novel widespread and sustained human-to-human context of the current monkeypox outbreak.
https://www.biorxiv.org/content/10.1101/2022.07.23.501239v1.full.pdf