tetano
Editor, Senior Moderator
Genome Announc. 2014 Oct 2;2(5). pii: e00841-14. doi: 10.1128/genomeA.00841-14.
Genetic Instability of Influenza pH1N1 Viruses.
Jalovaara P1, Bychkov D1, Ahtiainen L1, Kallio-Kokko H2, Valkonen M3, Kantele A3, Mattila P1, Almusa H1, Kallioniemi O1, Kainov D4.
Author information
Abstract
Here, we report full-length genome sequences of influenza pH1N1 viruses obtained prior to and after propagation in MDCK cells. Paired comparisons of the genomes showed that each strain acquired 1.0 to 18.8 mutations per genome per replication cycle, which corresponds to 0.5 to 5.8 mutations per virus proteome per replication cycle. Our analysis indicates that pH1N1 viruses accumulated adaptive mutations among others in response to propagation in cell culture. These results could be important for vaccine and drug-sensitivity surveillance studies, as well as for vaccine and antiviral drug development programs where cell cultures are used for influenza propagation.
Copyright ? 2014 Jalovaara et al.
PMID:
25278520
[PubMed]
PMCID:
PMC4183864
Free PMC Article
http://www.ncbi.nlm.nih.gov/pubmed/25278520
Genetic Instability of Influenza pH1N1 Viruses.
Jalovaara P1, Bychkov D1, Ahtiainen L1, Kallio-Kokko H2, Valkonen M3, Kantele A3, Mattila P1, Almusa H1, Kallioniemi O1, Kainov D4.
Author information
Abstract
Here, we report full-length genome sequences of influenza pH1N1 viruses obtained prior to and after propagation in MDCK cells. Paired comparisons of the genomes showed that each strain acquired 1.0 to 18.8 mutations per genome per replication cycle, which corresponds to 0.5 to 5.8 mutations per virus proteome per replication cycle. Our analysis indicates that pH1N1 viruses accumulated adaptive mutations among others in response to propagation in cell culture. These results could be important for vaccine and drug-sensitivity surveillance studies, as well as for vaccine and antiviral drug development programs where cell cultures are used for influenza propagation.
Copyright ? 2014 Jalovaara et al.
PMID:
25278520
[PubMed]
PMCID:
PMC4183864
Free PMC Article
http://www.ncbi.nlm.nih.gov/pubmed/25278520