tetano
Editor, Senior Moderator
Med Mal Infect. 2013 Jul 19. pii: S0399-077X(13)00168-6. doi: 10.1016/j.medmal.2013.06.005. [Epub ahead of print]
Genetic diversity of Influenza B virus in 2009-2010 and 2010-2011 in Tunisia.
El Moussi A, Ben Hadj Kacem MA, Ledesma J, Pozo F, Teresa Cuevas M, Casas I, Slim A.
Source
National Influenza Centre-Tunis, Unit Virology, Microbiology Laboratory, Charles Nicolle's Hospital, boulevard 9-Avril, 1006 Tunis, Tunisia. Electronic address: awatefbio@ymail.com.
Abstract
OBJECTIVE:
The authors had for aim to characterize influenza B strains having circulated in Tunisia to identify new mutations and compare them with reference strains.
METHODS:
The epidemiological surveillance of influenza allowed identifying 19 patients with symptoms related to respiratory infection, who had been infected by influenza B strains isolated in several regions of Tunisia in 2009-2010 and in 2010-2011. Laboratory identification and detection of mutations in the segment encoding hemagglutinin of influenza viruses was performed by real time PCR and sequencing.
RESULTS:
The two influenza B Tunisian strains of the 2009-2010 season belonged to the Victoria lineage, whereas 2010-2011 season strains belonged to B/Victoria/2/87 and B/Yamagata/16/88 lineages with a dominance of the Yamagata lineage (76%). This study allowed identifying amino acid substitutions: T121A, S150I, N165Y, T181A, G183R, D196N, S229D, M251V and K253R in the B/Yamagata lineage; L58P, N75K, K109N, N165K, S172P and K257R into the B/Victoria lineage. These mutations were specific of Tunisian groups of variants. Most influenza B-Yamagata lineage viruses (69%) were associated with severe cases.
CONCLUSION:
Molecular analysis of the various influenza B strains circulating in Tunisia is useful to detect new mutations that can modify the phenotype of influenza strains.
Copyright ? 2013 Elsevier Masson SAS. All rights reserved.
KEYWORDS:
B/Victoria lineage, B/Yamagata lineage, Grippe B, Influenza B, Lign?e B-Victoria, Lign?e B-Yamagata, Mutation, Phylogeny, Phylog?nie, Sequencing, S?quen?age
PMID:
23876206
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23876206
Genetic diversity of Influenza B virus in 2009-2010 and 2010-2011 in Tunisia.
El Moussi A, Ben Hadj Kacem MA, Ledesma J, Pozo F, Teresa Cuevas M, Casas I, Slim A.
Source
National Influenza Centre-Tunis, Unit Virology, Microbiology Laboratory, Charles Nicolle's Hospital, boulevard 9-Avril, 1006 Tunis, Tunisia. Electronic address: awatefbio@ymail.com.
Abstract
OBJECTIVE:
The authors had for aim to characterize influenza B strains having circulated in Tunisia to identify new mutations and compare them with reference strains.
METHODS:
The epidemiological surveillance of influenza allowed identifying 19 patients with symptoms related to respiratory infection, who had been infected by influenza B strains isolated in several regions of Tunisia in 2009-2010 and in 2010-2011. Laboratory identification and detection of mutations in the segment encoding hemagglutinin of influenza viruses was performed by real time PCR and sequencing.
RESULTS:
The two influenza B Tunisian strains of the 2009-2010 season belonged to the Victoria lineage, whereas 2010-2011 season strains belonged to B/Victoria/2/87 and B/Yamagata/16/88 lineages with a dominance of the Yamagata lineage (76%). This study allowed identifying amino acid substitutions: T121A, S150I, N165Y, T181A, G183R, D196N, S229D, M251V and K253R in the B/Yamagata lineage; L58P, N75K, K109N, N165K, S172P and K257R into the B/Victoria lineage. These mutations were specific of Tunisian groups of variants. Most influenza B-Yamagata lineage viruses (69%) were associated with severe cases.
CONCLUSION:
Molecular analysis of the various influenza B strains circulating in Tunisia is useful to detect new mutations that can modify the phenotype of influenza strains.
Copyright ? 2013 Elsevier Masson SAS. All rights reserved.
KEYWORDS:
B/Victoria lineage, B/Yamagata lineage, Grippe B, Influenza B, Lign?e B-Victoria, Lign?e B-Yamagata, Mutation, Phylogeny, Phylog?nie, Sequencing, S?quen?age
PMID:
23876206
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23876206