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Journal of Clinical Virology
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doi:10.1016/j.jcv.2011.09.032 | How to Cite or Link Using DOI
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Genetic diversity of influenza A(H1N1)2009 virus circulating during the season 2010?2011 in Spain
Juan Ledesmaa, Corresponding Author Contact Information, E-mail The Corresponding Author, Francisco Pozoa, Gabriel Reinab, Miriam Blascoc, Guadalupe Rodr?guezd, Milagrosa Montese, Isabel L?pez-Miragayaf, Carmen Salvadorg, Jordi Reinah, Ra?l Ort?z de Lejarazui, Pilar Egidoj, Jos? L?pez Barbak, Concepci?n Delgadol, Mar?a Teresa Cuevasa, Inmaculada Casasa and the Spanish Influenza Surveillance System (SISS)
a National Influenza Centre-Madrid, Influenza and Respiratory Viruses Laboratory, Instituto de Salud Carlos III, Madrid, Spain
b Servicio de Microbiolog?a, Cl?nica Universidad de Navarra, Pamplona, Navarra, Spain
c Servicio de Microbiolog?a, Hospital San Pedro, Logro?o, La Rioja, Spain
d Servicio de Mircrobiolog?a, Hospital San Pedro de Alc?ntara, C?ceres, Extremadura, Spain
e Servicio de Mircrobiolog?a, Hospital Donostia, Pa?s Vasco, Spain
f Servicio de Mircrobiolog?a del Complejo Hospitalario de Vigo, Galicia, Spain
g Servicio de Microbiolog?a, Hospital Virgen de la Arrixaca, Murcia, Spain
h Servicio de Microbiolog?a, Hospital Universitari Son Espases, Palma de Mallorca, Baleares, Spain
i National Influenza Centre-Valladolid, Facultad de Medicina, Valladolid, Castilla y Le?n, Spain
j Servicio de Microbiolog?a, Hospital Miguel Servet, Zaragoza, Arag?n, Spain
k Servicio de Microbiolog?a, Hospital de INGESA, Ceuta, Spain
l National Centre of Epidemiology, Instituto de Salud Carlos III, Madrid, Spain
Received 11 August 2011; revised 26 September 2011; Accepted 29 September 2011. Available online 8 November 2011.
Abstract
Background
Genetic diversity of influenza A(H1N1)2009 viruses has been reported since the pandemic virus emerged in April 2009. Different genetic clades have been identified and defined based on amino acid substitutions found in the haemagglutinin (HA) protein sequences. In Spain, circulating influenza viruses are monitored each season by the regional laboratories enrolled in the Spanish Influenza Surveillance System (SISS). The analysis of the HA gene sequence helps to detect the genetic diversity and viral evolution.
Objectives
To perform an analysis of the genetic diversity of influenza A(H1N1)2009 viruses circulating in Spain during the season 2010?2011 based on analysis of the HA sequence gene.
Study design
Phylogenetic analysis based on the HA1 subunit of the haemagglutinin gene was carried out on 220 influenza A(H1N1)2009 viruses circulating during the season 2010?2011.
Results
Six different genetic groups were identified among circulating A(H1N1)2009 viruses, five of them were previously reported during season 2010?2011. A new group, characterized by E172K and K308E changes and a proline at position 83, was observed in 12.27% of the Spanish viruses.
Conclusion
Co-circulation of six different genetic groups of influenza A(H1N1)2009 viruses was identified in Spain during the season 2010?2011. Nevertheless, at this stage, none of the groups identified to date have resulted in significant antigenic changes according to data collected by World Health Organization Collaborating Centres for influenza surveillance.
Abbreviations: HA, haemagglutinin; NA, neuraminidase; SISS, Spanish Influenza Surveillance System; WHO, World Health Organization; MDCK, Madin?Darby Canine Kidney Cells; S, serine; T, treonine; D, aspartic acid; N, asparagine; R, arginine; K, lysine; I, isoleucine; V, valine; L, leucine; E, glutamic acid; A, alanine; P, proline; G, glycine; Q, glutamine
http://www.sciencedirect.com/science/article/pii/S1386653211004161
In Press, Corrected Proof - Note to users
doi:10.1016/j.jcv.2011.09.032 | How to Cite or Link Using DOI
Permissions & Reprints
Genetic diversity of influenza A(H1N1)2009 virus circulating during the season 2010?2011 in Spain
Juan Ledesmaa, Corresponding Author Contact Information, E-mail The Corresponding Author, Francisco Pozoa, Gabriel Reinab, Miriam Blascoc, Guadalupe Rodr?guezd, Milagrosa Montese, Isabel L?pez-Miragayaf, Carmen Salvadorg, Jordi Reinah, Ra?l Ort?z de Lejarazui, Pilar Egidoj, Jos? L?pez Barbak, Concepci?n Delgadol, Mar?a Teresa Cuevasa, Inmaculada Casasa and the Spanish Influenza Surveillance System (SISS)
a National Influenza Centre-Madrid, Influenza and Respiratory Viruses Laboratory, Instituto de Salud Carlos III, Madrid, Spain
b Servicio de Microbiolog?a, Cl?nica Universidad de Navarra, Pamplona, Navarra, Spain
c Servicio de Microbiolog?a, Hospital San Pedro, Logro?o, La Rioja, Spain
d Servicio de Mircrobiolog?a, Hospital San Pedro de Alc?ntara, C?ceres, Extremadura, Spain
e Servicio de Mircrobiolog?a, Hospital Donostia, Pa?s Vasco, Spain
f Servicio de Mircrobiolog?a del Complejo Hospitalario de Vigo, Galicia, Spain
g Servicio de Microbiolog?a, Hospital Virgen de la Arrixaca, Murcia, Spain
h Servicio de Microbiolog?a, Hospital Universitari Son Espases, Palma de Mallorca, Baleares, Spain
i National Influenza Centre-Valladolid, Facultad de Medicina, Valladolid, Castilla y Le?n, Spain
j Servicio de Microbiolog?a, Hospital Miguel Servet, Zaragoza, Arag?n, Spain
k Servicio de Microbiolog?a, Hospital de INGESA, Ceuta, Spain
l National Centre of Epidemiology, Instituto de Salud Carlos III, Madrid, Spain
Received 11 August 2011; revised 26 September 2011; Accepted 29 September 2011. Available online 8 November 2011.
Abstract
Background
Genetic diversity of influenza A(H1N1)2009 viruses has been reported since the pandemic virus emerged in April 2009. Different genetic clades have been identified and defined based on amino acid substitutions found in the haemagglutinin (HA) protein sequences. In Spain, circulating influenza viruses are monitored each season by the regional laboratories enrolled in the Spanish Influenza Surveillance System (SISS). The analysis of the HA gene sequence helps to detect the genetic diversity and viral evolution.
Objectives
To perform an analysis of the genetic diversity of influenza A(H1N1)2009 viruses circulating in Spain during the season 2010?2011 based on analysis of the HA sequence gene.
Study design
Phylogenetic analysis based on the HA1 subunit of the haemagglutinin gene was carried out on 220 influenza A(H1N1)2009 viruses circulating during the season 2010?2011.
Results
Six different genetic groups were identified among circulating A(H1N1)2009 viruses, five of them were previously reported during season 2010?2011. A new group, characterized by E172K and K308E changes and a proline at position 83, was observed in 12.27% of the Spanish viruses.
Conclusion
Co-circulation of six different genetic groups of influenza A(H1N1)2009 viruses was identified in Spain during the season 2010?2011. Nevertheless, at this stage, none of the groups identified to date have resulted in significant antigenic changes according to data collected by World Health Organization Collaborating Centres for influenza surveillance.
Abbreviations: HA, haemagglutinin; NA, neuraminidase; SISS, Spanish Influenza Surveillance System; WHO, World Health Organization; MDCK, Madin?Darby Canine Kidney Cells; S, serine; T, treonine; D, aspartic acid; N, asparagine; R, arginine; K, lysine; I, isoleucine; V, valine; L, leucine; E, glutamic acid; A, alanine; P, proline; G, glycine; Q, glutamine
http://www.sciencedirect.com/science/article/pii/S1386653211004161