tetano
Editor, Senior Moderator
Influenza Other Respir Viruses. 2013 Dec 6. doi: 10.1111/irv.12219. [Epub ahead of print]
Genetic characterization of seasonal influenza A (H3N2) viruses in Ontario during 2010-2011 influenza season: high prevalence of mutations at antigenic sites.
Eshaghi A, Duvvuri VR, Li A, Patel SN, Bastien N, Li Y, Low DE, Gubbay JB.
Source
Ontario Agency for Health Protection and Promotion, Toronto, ON, Canada.
Abstract
BACKGROUND:
The direct effect of antigenic site mutations in influenza viruses on antigenic drift and vaccine effectiveness is poorly understood.
OBJECTIVE:
To investigate the genetic and antigenic characteristics of human influenza A (H3N2) viruses circulating in Ontario during the early 2010-2011 winter season.
STUDY DESIGN:
We sequenced the hemagglutinin (HA) and neuraminidase (NA) genes from 41 A(H3N2) viruses detected in nasopharyngeal specimens. Strain typing was performed by hemagglutination inhibition (HI) assay. Molecular and phylogenetic tree analyses were conducted.
RESULTS:
HA and NA genes showed high similarity to the 2010-2011 vaccine strain, A/Perth/16/2009 (H3N2)-like virus (97?7-98?5% and 98?7-99?5% amino acid (AA) identity, respectively). Compared to A/Perth/16/2009 strain, HA gene mutations were documented at 28 different AA positions across all five H3 antigenic sites, with a range of 5-11 mutations in individual viruses. Thirty-six (88%) viruses had 8 AA substitutions in common; none of these had reduced HI titer. Among Ontario isolates, 11 antigenic site AAs were positively selected with an increase in glycosylation sites.
CONCLUSION:
The presence of antigenic site mutations with high frequency among 2010-2011 influenza H3N2 isolates confirms ongoing adaptive H3N2 evolution. These may represent early phylogenetic changes that could cause antigenic drift with further mutations. Clinical relevance of antigenic site mutations not causing drift in HI assays is unknown and requires further investigation. In addition, viral sequencing information will assist with vaccine strain planning and may facilitate early detection of vaccine escape.
? 2013 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd.
KEYWORDS:
Antigenic site mutations, genetic and antigenic characterization, phylogenetic analysis, positive selection analysis, seasonal influenza A (H3N2) virus
PMID:
24313991
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24313991
Genetic characterization of seasonal influenza A (H3N2) viruses in Ontario during 2010-2011 influenza season: high prevalence of mutations at antigenic sites.
Eshaghi A, Duvvuri VR, Li A, Patel SN, Bastien N, Li Y, Low DE, Gubbay JB.
Source
Ontario Agency for Health Protection and Promotion, Toronto, ON, Canada.
Abstract
BACKGROUND:
The direct effect of antigenic site mutations in influenza viruses on antigenic drift and vaccine effectiveness is poorly understood.
OBJECTIVE:
To investigate the genetic and antigenic characteristics of human influenza A (H3N2) viruses circulating in Ontario during the early 2010-2011 winter season.
STUDY DESIGN:
We sequenced the hemagglutinin (HA) and neuraminidase (NA) genes from 41 A(H3N2) viruses detected in nasopharyngeal specimens. Strain typing was performed by hemagglutination inhibition (HI) assay. Molecular and phylogenetic tree analyses were conducted.
RESULTS:
HA and NA genes showed high similarity to the 2010-2011 vaccine strain, A/Perth/16/2009 (H3N2)-like virus (97?7-98?5% and 98?7-99?5% amino acid (AA) identity, respectively). Compared to A/Perth/16/2009 strain, HA gene mutations were documented at 28 different AA positions across all five H3 antigenic sites, with a range of 5-11 mutations in individual viruses. Thirty-six (88%) viruses had 8 AA substitutions in common; none of these had reduced HI titer. Among Ontario isolates, 11 antigenic site AAs were positively selected with an increase in glycosylation sites.
CONCLUSION:
The presence of antigenic site mutations with high frequency among 2010-2011 influenza H3N2 isolates confirms ongoing adaptive H3N2 evolution. These may represent early phylogenetic changes that could cause antigenic drift with further mutations. Clinical relevance of antigenic site mutations not causing drift in HI assays is unknown and requires further investigation. In addition, viral sequencing information will assist with vaccine strain planning and may facilitate early detection of vaccine escape.
? 2013 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd.
KEYWORDS:
Antigenic site mutations, genetic and antigenic characterization, phylogenetic analysis, positive selection analysis, seasonal influenza A (H3N2) virus
PMID:
24313991
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24313991