tetano
Editor, Senior Moderator
J Med Virol. 2018 Jul 23. doi: 10.1002/jmv.25273. [Epub ahead of print]
[h=1]Genetic characterization of influenza B virus in Cameroon and high frequency of reassortant strains.[/h] Monamele CG[SUP]1,[/SUP][SUP]2[/SUP], Vernet MA[SUP]1[/SUP], Njankouo MR[SUP]1[/SUP], Kenmoe S[SUP]1[/SUP], Schoenhals M[SUP]1[/SUP], Yahaya AA[SUP]3[/SUP], Anong DN[SUP]2,[/SUP][SUP]4[/SUP], Akoachere JF[SUP]2[/SUP], Njouom R[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza B is broadly divided into B/Victoria and B/Yamagata lineages based on their genetic and antigenic properties. We describe in this study the first report on genome characterization of type B influenza virus in the Cameroon National Influenza Centre (NIC) between 2014 and 2017. Respiratory samples were collected as part of the influenza surveillance activity in the NIC. RNA products were tested for the presence of influenza using the CDC Influenza A/B typing panel. Thirty-five samples positive for influenza B were selected for sequencing three gene segments (HA, NA and M) and phylogenetic trees were generated by MEGA version 6.0. Nucleotide phylogenetic analysis of the HA gene revealed the presence of three major clades among Cameroonian strains. All Victoria lineages grouped into B/Victoria clade 1A, while, Yamagata lineages grouped into Yamagata clade 2 (2014 strains) and Yamagata clade 3 (2015-2017). We observed a high frequency of reassortant viruses with Yamagata-like HA gene and Victoria-like NA gene (27.4%; 23/84). Results from this study confirm variations in the genome composition of type B influenza virus and emphasize on the relevance of molecular surveillance for spotting peculiar genetic variants of public health and clinical significance. This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Cameroon; Victoria lineage; Yamagata lineage; genome characterization; influenza B
PMID: 30036447 DOI: 10.1002/jmv.25273
[h=1]Genetic characterization of influenza B virus in Cameroon and high frequency of reassortant strains.[/h] Monamele CG[SUP]1,[/SUP][SUP]2[/SUP], Vernet MA[SUP]1[/SUP], Njankouo MR[SUP]1[/SUP], Kenmoe S[SUP]1[/SUP], Schoenhals M[SUP]1[/SUP], Yahaya AA[SUP]3[/SUP], Anong DN[SUP]2,[/SUP][SUP]4[/SUP], Akoachere JF[SUP]2[/SUP], Njouom R[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza B is broadly divided into B/Victoria and B/Yamagata lineages based on their genetic and antigenic properties. We describe in this study the first report on genome characterization of type B influenza virus in the Cameroon National Influenza Centre (NIC) between 2014 and 2017. Respiratory samples were collected as part of the influenza surveillance activity in the NIC. RNA products were tested for the presence of influenza using the CDC Influenza A/B typing panel. Thirty-five samples positive for influenza B were selected for sequencing three gene segments (HA, NA and M) and phylogenetic trees were generated by MEGA version 6.0. Nucleotide phylogenetic analysis of the HA gene revealed the presence of three major clades among Cameroonian strains. All Victoria lineages grouped into B/Victoria clade 1A, while, Yamagata lineages grouped into Yamagata clade 2 (2014 strains) and Yamagata clade 3 (2015-2017). We observed a high frequency of reassortant viruses with Yamagata-like HA gene and Victoria-like NA gene (27.4%; 23/84). Results from this study confirm variations in the genome composition of type B influenza virus and emphasize on the relevance of molecular surveillance for spotting peculiar genetic variants of public health and clinical significance. This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Cameroon; Victoria lineage; Yamagata lineage; genome characterization; influenza B
PMID: 30036447 DOI: 10.1002/jmv.25273