tetano
Editor, Senior Moderator
Curr Microbiol. 2017 May 11. doi: 10.1007/s00284-017-1262-6. [Epub ahead of print]
[h=1]Genetic Characterization of Influenza A (H1N1) Pandemic 2009 Virus Isolates from Mumbai.[/h] Gohil D[SUP]1[/SUP], Kothari S[SUP]2[/SUP], Shinde P[SUP]3[/SUP], Meharunkar R[SUP]4[/SUP], Warke R[SUP]2,[/SUP][SUP]4[/SUP], Chowdhary A[SUP]2[/SUP], Deshmukh R[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Pandemic influenza A (H1N1) 2009 virus was first detected in India in May 2009 which subsequently became endemic in many parts of the country. Influenza A viruses have the ability to evade the immune response through its ability of antigenic variations. The study aims to characterize influenza A (H1N1) pdm 09 viruses circulating in Mumbai during the pandemic and post-pandemic period. Nasopharyngeal swabs positive for influenza A (H1N1) pdm 09 viruses were inoculated on Madin-Darby canine kidney cell line for virus isolation. Molecular and phylogenetic analysis of influenza A (H1N1) pdm 09 isolates was conducted to understand the evolution and genetic diversity of the strains. Nucleotide and amino acid sequences of the HA gene of Mumbai isolates when compared to A/California/07/2009-vaccine strain revealed 14 specific amino acid differences located at the antigenic sites. Amino acid variations in HA and NA gene resulted in changes in the N-linked glycosylation motif which may lead to immune evasion. Phylogenetic analysis of the isolates revealed their evolutionary position with vaccine strain A/California/07/2009 but had undergone changes gradually. The findings in the present study confirm genetic variability of influenza viruses and highlight the importance of continuous surveillance during influenza outbreaks.
PMID: 28497210 DOI: 10.1007/s00284-017-1262-6
[h=1]Genetic Characterization of Influenza A (H1N1) Pandemic 2009 Virus Isolates from Mumbai.[/h] Gohil D[SUP]1[/SUP], Kothari S[SUP]2[/SUP], Shinde P[SUP]3[/SUP], Meharunkar R[SUP]4[/SUP], Warke R[SUP]2,[/SUP][SUP]4[/SUP], Chowdhary A[SUP]2[/SUP], Deshmukh R[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Pandemic influenza A (H1N1) 2009 virus was first detected in India in May 2009 which subsequently became endemic in many parts of the country. Influenza A viruses have the ability to evade the immune response through its ability of antigenic variations. The study aims to characterize influenza A (H1N1) pdm 09 viruses circulating in Mumbai during the pandemic and post-pandemic period. Nasopharyngeal swabs positive for influenza A (H1N1) pdm 09 viruses were inoculated on Madin-Darby canine kidney cell line for virus isolation. Molecular and phylogenetic analysis of influenza A (H1N1) pdm 09 isolates was conducted to understand the evolution and genetic diversity of the strains. Nucleotide and amino acid sequences of the HA gene of Mumbai isolates when compared to A/California/07/2009-vaccine strain revealed 14 specific amino acid differences located at the antigenic sites. Amino acid variations in HA and NA gene resulted in changes in the N-linked glycosylation motif which may lead to immune evasion. Phylogenetic analysis of the isolates revealed their evolutionary position with vaccine strain A/California/07/2009 but had undergone changes gradually. The findings in the present study confirm genetic variability of influenza viruses and highlight the importance of continuous surveillance during influenza outbreaks.
PMID: 28497210 DOI: 10.1007/s00284-017-1262-6