tetano
Editor, Senior Moderator
PeerJ. 2019 Dec 17;7:e8239. doi: 10.7717/peerj.8239. eCollection 2019. [h=1]Genetic and serologic surveillance of canine (CIV) and equine (EIV) influenza virus in Nuevo Le?n State, M?xico.[/h]
Plata-Hip?lito CB[SUP]1[/SUP], Cedillo-Rosales S[SUP]2[/SUP], Obreg?n-Mac?as N[SUP]3[/SUP], Hern?ndez-Luna CE[SUP]4[/SUP], Rodr?guez-Padilla C[SUP]1[/SUP], Tamez-Guerra RS[SUP]1[/SUP], Contreras-Cordero JF[SUP]1[/SUP].
[h=3]Author information[/h] 1 Universidad Aut?noma de Nuevo Le?n, Facultad de Ciencias Biol?gicas, Laboratorio de Inmunolog?a y Virolog?a, San Nicol?s de los Garza, Nuevo Le?n, M?xico. 2 Universidad Aut?noma de Nuevo Le?n, Facultad de Medicina Veterinaria y Zootecnia, Departamento de Virolog?a, Escobedo, Nuevo Le?n, M?xico. 3 Universidad Aut?noma de Nuevo Le?n, Facultad de Medicina Veterinaria y Zootecnia, Departamento de Grandes Especies, Escobedo, Nuevo Le?n, M?xico. 4 Universidad Aut?noma de Nuevo Le?n, Facultad de Ciencias Biol?gicas, Departamento de Qu?mica, San Nicol?s de los Garza, Nuevo Le?n, M?xico.
[h=3]Abstract[/h] [h=4]Background:[/h] Despite the uncontrolled distribution of the Influenza A virus through wild birds, the detection of canine influenza virus and equine influenza virus in Mexico was absent until now. Recently, outbreaks of equine and canine influenza have been reported around the world; the virus spreads quickly among animals and there is potential for zoonotic transmission.
[h=4]Methods:[/h] Amplification of the Influenza A virus matrix gene from necropsies, nasal and conjunctival swabs from trash service horses and pets/stray dogs was performed through RT-PCR. The seroprevalence was carried out through Sandwich enzyme-linked immunosorbent assay system using the M1 recombinant protein and polyclonal antibodies anti-M1.
[h=4]Results:[/h] The matrix gene was amplified from 13 (19.11%) nasal swabs, two (2.94%) conjunctival swabs and five (7.35%) lung necropsies, giving a total of 20 (29.41%) positive samples in a pet dog population. A total of six (75%) positive samples of equine nasal swab were amplified. Sequence analysis showed 96-99% identity with sequences of Influenza A virus matrix gene present in H1N1, H1N2 and H3N2 subtypes. The phylogenetic analysis of the sequences revealed higher identity with matrix gene sequences detected from zoonotic isolates of subtype H1N1/2009. The detection of anti-M1 antibodies in stray dogs showed a prevalence of 123 (100%) of the sampled population, whereas in horses, 114 (92.68%) positivity was obtained.
[h=4]Conclusion:[/h] The results unveil the prevalence of Influenza A virus in the population of horses and dogs in the state of Nuevo Leon, which could indicate a possible outbreak of equine and Canine Influenza in Mexico. We suggest that the prevalence of Influenza virus in companion animals be monitored to investigate its epizootic and zoonotic potential, in addition to encouraging the regulation of vaccination in these animal species in order to improve their quality of life.
? 2019 Plata-Hip?lito et al.
[h=4]KEYWORDS:[/h] Canine Influenza Virus (CIV); Equine Influenza Virus (EIV); Matrix gene (M); Polyclonal antibodies
PMID: 31871842 PMCID: PMC6924343 DOI: 10.7717/peerj.8239
Free PMC Article
Plata-Hip?lito CB[SUP]1[/SUP], Cedillo-Rosales S[SUP]2[/SUP], Obreg?n-Mac?as N[SUP]3[/SUP], Hern?ndez-Luna CE[SUP]4[/SUP], Rodr?guez-Padilla C[SUP]1[/SUP], Tamez-Guerra RS[SUP]1[/SUP], Contreras-Cordero JF[SUP]1[/SUP].
[h=3]Author information[/h] 1 Universidad Aut?noma de Nuevo Le?n, Facultad de Ciencias Biol?gicas, Laboratorio de Inmunolog?a y Virolog?a, San Nicol?s de los Garza, Nuevo Le?n, M?xico. 2 Universidad Aut?noma de Nuevo Le?n, Facultad de Medicina Veterinaria y Zootecnia, Departamento de Virolog?a, Escobedo, Nuevo Le?n, M?xico. 3 Universidad Aut?noma de Nuevo Le?n, Facultad de Medicina Veterinaria y Zootecnia, Departamento de Grandes Especies, Escobedo, Nuevo Le?n, M?xico. 4 Universidad Aut?noma de Nuevo Le?n, Facultad de Ciencias Biol?gicas, Departamento de Qu?mica, San Nicol?s de los Garza, Nuevo Le?n, M?xico.
[h=3]Abstract[/h] [h=4]Background:[/h] Despite the uncontrolled distribution of the Influenza A virus through wild birds, the detection of canine influenza virus and equine influenza virus in Mexico was absent until now. Recently, outbreaks of equine and canine influenza have been reported around the world; the virus spreads quickly among animals and there is potential for zoonotic transmission.
[h=4]Methods:[/h] Amplification of the Influenza A virus matrix gene from necropsies, nasal and conjunctival swabs from trash service horses and pets/stray dogs was performed through RT-PCR. The seroprevalence was carried out through Sandwich enzyme-linked immunosorbent assay system using the M1 recombinant protein and polyclonal antibodies anti-M1.
[h=4]Results:[/h] The matrix gene was amplified from 13 (19.11%) nasal swabs, two (2.94%) conjunctival swabs and five (7.35%) lung necropsies, giving a total of 20 (29.41%) positive samples in a pet dog population. A total of six (75%) positive samples of equine nasal swab were amplified. Sequence analysis showed 96-99% identity with sequences of Influenza A virus matrix gene present in H1N1, H1N2 and H3N2 subtypes. The phylogenetic analysis of the sequences revealed higher identity with matrix gene sequences detected from zoonotic isolates of subtype H1N1/2009. The detection of anti-M1 antibodies in stray dogs showed a prevalence of 123 (100%) of the sampled population, whereas in horses, 114 (92.68%) positivity was obtained.
[h=4]Conclusion:[/h] The results unveil the prevalence of Influenza A virus in the population of horses and dogs in the state of Nuevo Leon, which could indicate a possible outbreak of equine and Canine Influenza in Mexico. We suggest that the prevalence of Influenza virus in companion animals be monitored to investigate its epizootic and zoonotic potential, in addition to encouraging the regulation of vaccination in these animal species in order to improve their quality of life.
? 2019 Plata-Hip?lito et al.
[h=4]KEYWORDS:[/h] Canine Influenza Virus (CIV); Equine Influenza Virus (EIV); Matrix gene (M); Polyclonal antibodies
PMID: 31871842 PMCID: PMC6924343 DOI: 10.7717/peerj.8239
Free PMC Article