tetano
Editor, Senior Moderator
J Gen Virol. 2010 Dec 22. [Epub ahead of print]
Genetic and antigenic characterization of H1 influenza viruses from United States swine from 2008.
Lorusso A, Vincent AL, Harland ML, Alt D, Bayles DO, Swenson SL, Gramer MR, Russell CA, Smith DJ, Lager KM, Lewis NS.
Virus and Prion Disease Research Unit, USDA/ARS/NADC, Ames, IA, USA;
Abstract
Prior to the introduction of the 2009 pandemic H1N1 virus from humans into pigs, four phylogenetic clusters (α-, β-, γ- and δ) of the hemagglutinin (HA) gene from H1 influenza viruses could be found in U.S. swine. Information regarding the antigenic relatedness of the H1 viruses was lacking due to the dynamic and variable nature of swine lineage H1. We characterized twelve H1 isolates from 2008 by using 454 genome sequencing technology and phylogenetic analysis of all eight gene segments and by serologic cross-reactivity in the hemagglutination inhibition (HI) assay. Genetic diversity was demonstrated in all gene segments, but most notably in the HA gene. The gene segments from the 2009 pandemic H1N1 formed clusters separate from North American swine lineage viruses, suggesting progenitors of the pandemic virus were not present in U.S. pigs immediately prior to 2009. Serologic cross-reactivity paired with antigenic cartography demonstrated that the viruses in the different phylogenetic clusters are also antigenically divergent.
PMID: 21177926 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21177926
Genetic and antigenic characterization of H1 influenza viruses from United States swine from 2008.
Lorusso A, Vincent AL, Harland ML, Alt D, Bayles DO, Swenson SL, Gramer MR, Russell CA, Smith DJ, Lager KM, Lewis NS.
Virus and Prion Disease Research Unit, USDA/ARS/NADC, Ames, IA, USA;
Abstract
Prior to the introduction of the 2009 pandemic H1N1 virus from humans into pigs, four phylogenetic clusters (α-, β-, γ- and δ) of the hemagglutinin (HA) gene from H1 influenza viruses could be found in U.S. swine. Information regarding the antigenic relatedness of the H1 viruses was lacking due to the dynamic and variable nature of swine lineage H1. We characterized twelve H1 isolates from 2008 by using 454 genome sequencing technology and phylogenetic analysis of all eight gene segments and by serologic cross-reactivity in the hemagglutination inhibition (HI) assay. Genetic diversity was demonstrated in all gene segments, but most notably in the HA gene. The gene segments from the 2009 pandemic H1N1 formed clusters separate from North American swine lineage viruses, suggesting progenitors of the pandemic virus were not present in U.S. pigs immediately prior to 2009. Serologic cross-reactivity paired with antigenic cartography demonstrated that the viruses in the different phylogenetic clusters are also antigenically divergent.
PMID: 21177926 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21177926