tetano
Editor, Senior Moderator
J Med Virol. 2016 Jul 6. doi: 10.1002/jmv.24625. [Epub ahead of print]
[h=1]Genetic analysis of neuraminidase gene of influenza A(H1N1)pdm09 virus circulating in Southwest India from 2009-2012.[/h] Jagadesh A[SUP]1[/SUP], Salam AA[SUP]2[/SUP], Zadeh VR[SUP]1[/SUP], Arunkumar G[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Genetic analysis of neuraminidase gene sequences in 23 archived isolates of influenza A(H1N1)pdm09 virus, isolated during the 2009-2012 influenza seasons, was carried out to determine the genetic variability. Amino acid substitutions were observed at the rates of 0.3-0.7% per year. The catalytic site consisting of eight functional and 11 framework residues were found conserved in 20 isolates and mutated in three (E228G, E278G and N295T) isolates. To the best of our knowledge the three catalytic site mutants observed in our study have not been reported elsewhere to date. Similarly, mutations in the antigenic sites (K217E, K254E, V267A and D451E except I263V) are discussed for the first time through this article. The effect of these mutations on drug and antibody binding were analyzed using biochemical and structural studies. Detailed studies on the neuraminidase gene are sparse and our study may serve as an appropriate platform to gain insights about the evolution of influenza virus, thereby facilitating drugs/vaccines design and development. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Antigenic site; Catalytic site; Genetic analysis; Influenza virus; Neuraminidase
PMID: 27380821 DOI: 10.1002/jmv.24625
[PubMed - as supplied by publisher]
[h=1]Genetic analysis of neuraminidase gene of influenza A(H1N1)pdm09 virus circulating in Southwest India from 2009-2012.[/h] Jagadesh A[SUP]1[/SUP], Salam AA[SUP]2[/SUP], Zadeh VR[SUP]1[/SUP], Arunkumar G[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Genetic analysis of neuraminidase gene sequences in 23 archived isolates of influenza A(H1N1)pdm09 virus, isolated during the 2009-2012 influenza seasons, was carried out to determine the genetic variability. Amino acid substitutions were observed at the rates of 0.3-0.7% per year. The catalytic site consisting of eight functional and 11 framework residues were found conserved in 20 isolates and mutated in three (E228G, E278G and N295T) isolates. To the best of our knowledge the three catalytic site mutants observed in our study have not been reported elsewhere to date. Similarly, mutations in the antigenic sites (K217E, K254E, V267A and D451E except I263V) are discussed for the first time through this article. The effect of these mutations on drug and antibody binding were analyzed using biochemical and structural studies. Detailed studies on the neuraminidase gene are sparse and our study may serve as an appropriate platform to gain insights about the evolution of influenza virus, thereby facilitating drugs/vaccines design and development. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Antigenic site; Catalytic site; Genetic analysis; Influenza virus; Neuraminidase
PMID: 27380821 DOI: 10.1002/jmv.24625
[PubMed - as supplied by publisher]