tetano
Editor, Senior Moderator
J Am Chem Soc. 2011 May 11. [Epub ahead of print]
Genetic Analysis of H1N1 Influenza Virus from Throat Swab Samples in a Microfluidic System for Point-of-Care Diagnostics.
Ferguson BS, Buchsbaum SF, Wu TT, Hsieh K, Xiao Y, Sun R, Soh HT.
Abstract
The ability to obtain sequence-specific genetic information about rare target organisms directly from complex biological samples at the point of care would transform many areas of biotechnology. Microfluidics technology offers compelling tools for integrating multiple biochemical processes in a single device, but despite significant progress, only limited examples have shown specific, genetic analysis of clinical samples within the context of a fully integrated, portable platform. Herein we present the Magnetic Integrated Microfluidic Electrochemical Detector (MIMED) that integrates sample preparation and electrochemical sensors in a monolithic disposable device to detect RNA-based virus directly from patient samples. By combining immunomagnetic target capture, concentration and purification, reverse-transcriptase polymerase chain reaction (RT-PCR) and single-stranded DNA (ssDNA) generation in the sample preparation chamber, as well as sequence specific electrochemical DNA detection in the electrochemical cell, we demonstrate the detection of influenza H1N1 in throat swab samples at loads as low as 10 TCID50 - 4 orders of magnitude below the clinical titer for this virus. Given the availability of affinity reagents for a broad range of pathogens, our system offers a general approach for multi-target diagnostics at the point-of-care.
PMID:
21561070
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21561070
Genetic Analysis of H1N1 Influenza Virus from Throat Swab Samples in a Microfluidic System for Point-of-Care Diagnostics.
Ferguson BS, Buchsbaum SF, Wu TT, Hsieh K, Xiao Y, Sun R, Soh HT.
Abstract
The ability to obtain sequence-specific genetic information about rare target organisms directly from complex biological samples at the point of care would transform many areas of biotechnology. Microfluidics technology offers compelling tools for integrating multiple biochemical processes in a single device, but despite significant progress, only limited examples have shown specific, genetic analysis of clinical samples within the context of a fully integrated, portable platform. Herein we present the Magnetic Integrated Microfluidic Electrochemical Detector (MIMED) that integrates sample preparation and electrochemical sensors in a monolithic disposable device to detect RNA-based virus directly from patient samples. By combining immunomagnetic target capture, concentration and purification, reverse-transcriptase polymerase chain reaction (RT-PCR) and single-stranded DNA (ssDNA) generation in the sample preparation chamber, as well as sequence specific electrochemical DNA detection in the electrochemical cell, we demonstrate the detection of influenza H1N1 in throat swab samples at loads as low as 10 TCID50 - 4 orders of magnitude below the clinical titer for this virus. Given the availability of affinity reagents for a broad range of pathogens, our system offers a general approach for multi-target diagnostics at the point-of-care.
PMID:
21561070
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21561070