tetano
Editor, Senior Moderator
Genes Genomics
. 2023 May 3.
doi: 10.1007/s13258-023-01384-8. Online ahead of print. Host factor TNK2 is required for influenza virus infection
Ao Zhou[SUP] 1 2 [/SUP], Wenhua Zhang[SUP] 3 4 [/SUP], Baoxin Wang[SUP] 3 4 [/SUP]
Affiliations
Background: Host factors are required for Influenza virus infection and have great potential to become antiviral target.
Objective: Here we demonstrate the role of TNK2 in influenza virus infection. CRISPR/Cas9 induced TNK2 deletion in A549 cells.
Methods: CRISPR/Cas9-mediated deletion of TNK2. Western blotting and qPCR was used to measure the expression of TNK2 and other proteins.
Results: CRISPR/Cas9-mediated deletion of TNK2 decreased the replication of influenza virus and significantly inhibited the ex-pression of viral proteins and TNK2 inhibitors (XMD8-87 and AIM-100) reduced the expression of influenza M2, while over-expression of TNK2 weakened the resistance of TNK2-knockout cells to influenza virus infection. Furthermore, a decrease of nuclear import of IAV in the infected TNK2 mutant cells was observed in 3 h post-infection. Interestingly, TNK2 deletion enhanced the colocalization of LC3 with autophagic receptor p62 and led to the attenuation of influenza virus-caused accumulation of autophagosomes in TNK2 mutant cells. Further, confocal microscopy visualization result showed that influenza viral matrix 2 (M2) was colocalized with Lamp1 in the infected TNK2 mutant cells in early infection, while almost no colocalization between M2 and Lamp1 was observed in IAV-infected wild-type cells. Moreover, TNK2 depletion also affected the trafficking of early endosome and the movement of influenza viral NP and M2.
Conclusion: Our results identified TNK2 as a critical host factor for influenza viral M2 protein trafficking, suggesting that TNK2 will be an attractive target for the development of antivirals therapeutics.
Keywords: Autophagy; CRISPR; Influenza virus; Lysosome; TNK2.
. 2023 May 3.
doi: 10.1007/s13258-023-01384-8. Online ahead of print. Host factor TNK2 is required for influenza virus infection
Ao Zhou[SUP] 1 2 [/SUP], Wenhua Zhang[SUP] 3 4 [/SUP], Baoxin Wang[SUP] 3 4 [/SUP]
Affiliations
- PMID: 37133719
- DOI: 10.1007/s13258-023-01384-8
Background: Host factors are required for Influenza virus infection and have great potential to become antiviral target.
Objective: Here we demonstrate the role of TNK2 in influenza virus infection. CRISPR/Cas9 induced TNK2 deletion in A549 cells.
Methods: CRISPR/Cas9-mediated deletion of TNK2. Western blotting and qPCR was used to measure the expression of TNK2 and other proteins.
Results: CRISPR/Cas9-mediated deletion of TNK2 decreased the replication of influenza virus and significantly inhibited the ex-pression of viral proteins and TNK2 inhibitors (XMD8-87 and AIM-100) reduced the expression of influenza M2, while over-expression of TNK2 weakened the resistance of TNK2-knockout cells to influenza virus infection. Furthermore, a decrease of nuclear import of IAV in the infected TNK2 mutant cells was observed in 3 h post-infection. Interestingly, TNK2 deletion enhanced the colocalization of LC3 with autophagic receptor p62 and led to the attenuation of influenza virus-caused accumulation of autophagosomes in TNK2 mutant cells. Further, confocal microscopy visualization result showed that influenza viral matrix 2 (M2) was colocalized with Lamp1 in the infected TNK2 mutant cells in early infection, while almost no colocalization between M2 and Lamp1 was observed in IAV-infected wild-type cells. Moreover, TNK2 depletion also affected the trafficking of early endosome and the movement of influenza viral NP and M2.
Conclusion: Our results identified TNK2 as a critical host factor for influenza viral M2 protein trafficking, suggesting that TNK2 will be an attractive target for the development of antivirals therapeutics.
Keywords: Autophagy; CRISPR; Influenza virus; Lysosome; TNK2.