tetano
Editor, Senior Moderator
Front Immunol. 2019 Nov 6;10:2568. doi: 10.3389/fimmu.2019.02568. eCollection 2019. [h=1]Gene Expression-Based Identification of Antigen-Responsive CD8[SUP]+[/SUP] T Cells on a Single-Cell Level.[/h]
Fuchs YF[SUP]1[/SUP], Sharma V[SUP]1,[/SUP][SUP]2[/SUP], Eugster A[SUP]1[/SUP], Kraus G[SUP]1[/SUP], Morgenstern R[SUP]1[/SUP], Dahl A[SUP]3[/SUP], Reinhardt S[SUP]3[/SUP], Petzold A[SUP]3[/SUP], Lindner A[SUP]1[/SUP], L?bel D[SUP]1[/SUP], Bonifacio E[SUP]1,[/SUP][SUP]2,[/SUP][SUP]4[/SUP].
[h=3]Author information[/h] 1 Faculty of Medicine, DFG Center for Regenerative Therapies Dresden, Technische Universit?t Dresden, Dresden, Germany. 2 German Center for Diabetes Research (DZD), Paul Langerhans Institute Dresden, Technische Universit?t Dresden, Dresden, Germany. 3 DRESDEN-Concept Genome Center c/o Center for Molecular and Cellular Bioengineering, Technische Universit?t Dresden, Dresden, Germany. 4 Institute of Diabetes and Obesity, Helmholtz Zentrum M?nchen, German Research Center for Environmental Health, Neuherberg, Germany.
[h=3]Abstract[/h] CD8[SUP]+[/SUP] T cells are important effectors of adaptive immunity against pathogens, tumors, and self antigens. Here, we asked how human cognate antigen-responsive CD8[SUP]+[/SUP] T cells and their receptors could be identified in unselected single-cell gene expression data. Single-cell RNA sequencing and qPCR of dye-labeled antigen-specific cells identified large gene sets that were congruently up- or downregulated in virus-responsive CD8[SUP]+[/SUP] T cells under different antigen presentation conditions. Combined expression of TNFRSF9, XCL1, XCL2, and CRTAM was the most distinct marker of virus-responsive cells on a single-cell level. Using transcriptomic data, we developed a machine learning-based classifier that provides sensitive and specific detection of virus-responsive CD8[SUP]+[/SUP] T cells from unselected populations. Gene response profiles of CD8[SUP]+[/SUP] T cells specific for the autoantigen islet-specific glucose-6-phosphatase catalytic subunit-related protein differed markedly from virus-specific cells. These findings provide single-cell gene expression parameters for comprehensive identification of rare antigen-responsive cells and T cell receptors.
Copyright ? 2019 Fuchs, Sharma, Eugster, Kraus, Morgenstern, Dahl, Reinhardt, Petzold, Lindner, L?bel and Bonifacio.
[h=4]KEYWORDS:[/h] CD8+ T cells; CMV pp65; CTL (cytotoxic T lymphocyte); T cell receptor (TCR); antigen-responsive; gene-expression analysis; influenza matrix protein; single-cell
PMID: 31781096 PMCID: PMC6851025 DOI: 10.3389/fimmu.2019.02568
Free PMC Article
Fuchs YF[SUP]1[/SUP], Sharma V[SUP]1,[/SUP][SUP]2[/SUP], Eugster A[SUP]1[/SUP], Kraus G[SUP]1[/SUP], Morgenstern R[SUP]1[/SUP], Dahl A[SUP]3[/SUP], Reinhardt S[SUP]3[/SUP], Petzold A[SUP]3[/SUP], Lindner A[SUP]1[/SUP], L?bel D[SUP]1[/SUP], Bonifacio E[SUP]1,[/SUP][SUP]2,[/SUP][SUP]4[/SUP].
[h=3]Author information[/h] 1 Faculty of Medicine, DFG Center for Regenerative Therapies Dresden, Technische Universit?t Dresden, Dresden, Germany. 2 German Center for Diabetes Research (DZD), Paul Langerhans Institute Dresden, Technische Universit?t Dresden, Dresden, Germany. 3 DRESDEN-Concept Genome Center c/o Center for Molecular and Cellular Bioengineering, Technische Universit?t Dresden, Dresden, Germany. 4 Institute of Diabetes and Obesity, Helmholtz Zentrum M?nchen, German Research Center for Environmental Health, Neuherberg, Germany.
[h=3]Abstract[/h] CD8[SUP]+[/SUP] T cells are important effectors of adaptive immunity against pathogens, tumors, and self antigens. Here, we asked how human cognate antigen-responsive CD8[SUP]+[/SUP] T cells and their receptors could be identified in unselected single-cell gene expression data. Single-cell RNA sequencing and qPCR of dye-labeled antigen-specific cells identified large gene sets that were congruently up- or downregulated in virus-responsive CD8[SUP]+[/SUP] T cells under different antigen presentation conditions. Combined expression of TNFRSF9, XCL1, XCL2, and CRTAM was the most distinct marker of virus-responsive cells on a single-cell level. Using transcriptomic data, we developed a machine learning-based classifier that provides sensitive and specific detection of virus-responsive CD8[SUP]+[/SUP] T cells from unselected populations. Gene response profiles of CD8[SUP]+[/SUP] T cells specific for the autoantigen islet-specific glucose-6-phosphatase catalytic subunit-related protein differed markedly from virus-specific cells. These findings provide single-cell gene expression parameters for comprehensive identification of rare antigen-responsive cells and T cell receptors.
Copyright ? 2019 Fuchs, Sharma, Eugster, Kraus, Morgenstern, Dahl, Reinhardt, Petzold, Lindner, L?bel and Bonifacio.
[h=4]KEYWORDS:[/h] CD8+ T cells; CMV pp65; CTL (cytotoxic T lymphocyte); T cell receptor (TCR); antigen-responsive; gene-expression analysis; influenza matrix protein; single-cell
PMID: 31781096 PMCID: PMC6851025 DOI: 10.3389/fimmu.2019.02568
Free PMC Article