tetano
Editor, Senior Moderator
Future Microbiol
. 2020 Sep;15:1343-1352.
doi: 10.2217/fmb-2020-0149.
Mutation profile of over 4500 SARS-CoV-2 isolations reveals prevalent cytosine-to-uridine deamination on viral RNAs
Yue Li[SUP] 1 [/SUP], Xinai Yang[SUP] 1 [/SUP], Na Wang[SUP] 1 [/SUP], Haiyan Wang[SUP] 1 [/SUP], Bin Yin[SUP] 1 [/SUP], Xiaoping Yang[SUP] 1 [/SUP], Wenqing Jiang[SUP] 1 [/SUP]
Affiliations
Abstract
Aim: The inference of coronavirus evolution is largely based on mutations in SARS-CoV-2 genome. Misinterpretation of these mutations would mislead people about the evolution of SARS-CoV-2. Materials & methods: With 4521 lines of SARS-CoV-2, we obtained 3169 unique point mutation sites. We counted the numbers and calculated the minor allele frequency (MAF) of each mutation type. Results: Nearly half of the point mutations are C-T mismatches and 20% are A-G mismatches. The MAF of C-T and A-G mismatches is significantly higher than MAF of other mutation types. Conclusion: The excessive C-T mismatches do not resemble the random mutation profile. They are likely to be caused by the cytosine-to-uridine deamination system in hosts.
Keywords: MAF (minor allele frequency); RNA virus; SARS-CoV-2; deamination; mutations.
. 2020 Sep;15:1343-1352.
doi: 10.2217/fmb-2020-0149.
Mutation profile of over 4500 SARS-CoV-2 isolations reveals prevalent cytosine-to-uridine deamination on viral RNAs
Yue Li[SUP] 1 [/SUP], Xinai Yang[SUP] 1 [/SUP], Na Wang[SUP] 1 [/SUP], Haiyan Wang[SUP] 1 [/SUP], Bin Yin[SUP] 1 [/SUP], Xiaoping Yang[SUP] 1 [/SUP], Wenqing Jiang[SUP] 1 [/SUP]
Affiliations
- PMID: 33085541
- DOI: 10.2217/fmb-2020-0149
Abstract
Aim: The inference of coronavirus evolution is largely based on mutations in SARS-CoV-2 genome. Misinterpretation of these mutations would mislead people about the evolution of SARS-CoV-2. Materials & methods: With 4521 lines of SARS-CoV-2, we obtained 3169 unique point mutation sites. We counted the numbers and calculated the minor allele frequency (MAF) of each mutation type. Results: Nearly half of the point mutations are C-T mismatches and 20% are A-G mismatches. The MAF of C-T and A-G mismatches is significantly higher than MAF of other mutation types. Conclusion: The excessive C-T mismatches do not resemble the random mutation profile. They are likely to be caused by the cytosine-to-uridine deamination system in hosts.
Keywords: MAF (minor allele frequency); RNA virus; SARS-CoV-2; deamination; mutations.