tetano
Editor, Senior Moderator
Front Microbiol
. 2022 Jul 27;13:912138.
doi: 10.3389/fmicb.2022.912138. eCollection 2022.
A SARS-CoV-2 Negative Antigen Rapid Diagnostic in RT-qPCR Positive Samples Correlates With a Low Likelihood of Infectious Viruses in the Nasopharynx
Isadora Alonso Corrêa[SUP] 1 [/SUP], Débora Souza Faffe[SUP] 2 [/SUP], Rafael Mello Galliez[SUP] 3 [/SUP], Cássia Cristina Alves Gonçalves[SUP] 4 [/SUP], Richard Araújo Maia[SUP] 4 [/SUP], Gustavo Peixoto da Silva[SUP] 1 [/SUP], Filipe Romero Rebello Moreira[SUP] 4 [/SUP], Diana Mariani[SUP] 4 [/SUP], Mariana Freire Campos[SUP] 2 [/SUP], Isabela de Carvalho Leitão[SUP] 2 [/SUP], Marcos Romário de Souza[SUP] 1 [/SUP], Marcela Sabino Cunha[SUP] 1 [/SUP], Érica Ramos Dos Santos Nascimento[SUP] 4 [/SUP], Liane de Jesus Ribeiro[SUP] 4 [/SUP], Thais Felix Cordeiro da Cruz[SUP] 4 [/SUP], Cintia Policarpo[SUP] 4 [/SUP], Luis Gonzales[SUP] 5 [/SUP], Mary A Rodgers[SUP] 5 [/SUP], Michael Berg[SUP] 5 [/SUP], Roy Vijesurier[SUP] 5 [/SUP], Gavin A Cloherty[SUP] 5 [/SUP], John Hackett Jr[SUP] 5 [/SUP], Orlando da Costa Ferreira Jr[SUP] 4 [/SUP], Terezinha Marta Pereira Pinto Castiñeiras[SUP] 3 [/SUP], Amilcar Tanuri[SUP] 4 [/SUP], Luciana Jesus da Costa[SUP] 1 [/SUP]
Affiliations
Abstract
Severe acute respiratory syndrome-related coronavirus (SARS-CoV-2) transmission occurs even among fully vaccinated individuals; thus, prompt identification of infected patients is central to control viral circulation. Antigen rapid diagnostic tests (Ag-RDTs) are highly specific, but sensitivity is variable. Discordant RT-qPCR vs. Ag-RDT results are reported, raising the question of whether negative Ag-RDT in positive RT-qPCR samples could imply the absence of infectious viruses. To study the relationship between negative Ag-RDT results with virological, molecular, and serological parameters, we selected a cross-sectional and a follow-up dataset and analyzed virus culture, subgenomic RNA quantification, and sequencing to determine infectious viruses and mutations. We demonstrated that RT-qPCR positive while SARS-CoV-2 Ag-RDT negative discordant results correlate with the absence of infectious virus in nasopharyngeal samples. A decrease in sgRNA detection together with an expected increase in detectable anti-S and anti-N IgGs was also verified in these samples. The data clearly demonstrate that a negative Ag-RDT sample is less likely to harbor infectious SARS-CoV-2 and, consequently, has a lower transmissible potential.
Keywords: SARS-CoV-2; antigen rapid test; infectious virus detection; qRT- PCR; virus transmissibility.
. 2022 Jul 27;13:912138.
doi: 10.3389/fmicb.2022.912138. eCollection 2022.
A SARS-CoV-2 Negative Antigen Rapid Diagnostic in RT-qPCR Positive Samples Correlates With a Low Likelihood of Infectious Viruses in the Nasopharynx
Isadora Alonso Corrêa[SUP] 1 [/SUP], Débora Souza Faffe[SUP] 2 [/SUP], Rafael Mello Galliez[SUP] 3 [/SUP], Cássia Cristina Alves Gonçalves[SUP] 4 [/SUP], Richard Araújo Maia[SUP] 4 [/SUP], Gustavo Peixoto da Silva[SUP] 1 [/SUP], Filipe Romero Rebello Moreira[SUP] 4 [/SUP], Diana Mariani[SUP] 4 [/SUP], Mariana Freire Campos[SUP] 2 [/SUP], Isabela de Carvalho Leitão[SUP] 2 [/SUP], Marcos Romário de Souza[SUP] 1 [/SUP], Marcela Sabino Cunha[SUP] 1 [/SUP], Érica Ramos Dos Santos Nascimento[SUP] 4 [/SUP], Liane de Jesus Ribeiro[SUP] 4 [/SUP], Thais Felix Cordeiro da Cruz[SUP] 4 [/SUP], Cintia Policarpo[SUP] 4 [/SUP], Luis Gonzales[SUP] 5 [/SUP], Mary A Rodgers[SUP] 5 [/SUP], Michael Berg[SUP] 5 [/SUP], Roy Vijesurier[SUP] 5 [/SUP], Gavin A Cloherty[SUP] 5 [/SUP], John Hackett Jr[SUP] 5 [/SUP], Orlando da Costa Ferreira Jr[SUP] 4 [/SUP], Terezinha Marta Pereira Pinto Castiñeiras[SUP] 3 [/SUP], Amilcar Tanuri[SUP] 4 [/SUP], Luciana Jesus da Costa[SUP] 1 [/SUP]
Affiliations
- PMID: 35966714
- PMCID: PMC9364907
- DOI: 10.3389/fmicb.2022.912138
Abstract
Severe acute respiratory syndrome-related coronavirus (SARS-CoV-2) transmission occurs even among fully vaccinated individuals; thus, prompt identification of infected patients is central to control viral circulation. Antigen rapid diagnostic tests (Ag-RDTs) are highly specific, but sensitivity is variable. Discordant RT-qPCR vs. Ag-RDT results are reported, raising the question of whether negative Ag-RDT in positive RT-qPCR samples could imply the absence of infectious viruses. To study the relationship between negative Ag-RDT results with virological, molecular, and serological parameters, we selected a cross-sectional and a follow-up dataset and analyzed virus culture, subgenomic RNA quantification, and sequencing to determine infectious viruses and mutations. We demonstrated that RT-qPCR positive while SARS-CoV-2 Ag-RDT negative discordant results correlate with the absence of infectious virus in nasopharyngeal samples. A decrease in sgRNA detection together with an expected increase in detectable anti-S and anti-N IgGs was also verified in these samples. The data clearly demonstrate that a negative Ag-RDT sample is less likely to harbor infectious SARS-CoV-2 and, consequently, has a lower transmissible potential.
Keywords: SARS-CoV-2; antigen rapid test; infectious virus detection; qRT- PCR; virus transmissibility.