tetano
Editor, Senior Moderator
Front Med (Lausanne)
. 2022 Mar 15;9:835168.
doi: 10.3389/fmed.2022.835168. eCollection 2022.
Prolonged Shedding of SARS-CoV-2 in Feces of COVID-19 Positive Patients: Trends in Genomic Variation in First and Second Wave
Mallika Lavania[SUP] 1 [/SUP], Madhuri S Joshi[SUP] 1 [/SUP], Sujata S Ranshing[SUP] 1 [/SUP], Varsha A Potdar[SUP] 2 [/SUP], Manohar Shinde[SUP] 1 [/SUP], Nutan Chavan[SUP] 1 [/SUP], Santosh M Jadhav[SUP] 3 [/SUP], Prasad Sarkale[SUP] 4 [/SUP], Sreelekshmy Mohandas[SUP] 4 [/SUP], Pradeep M Sawant[SUP] 1 [/SUP], Sanjaykumar Tikute[SUP] 1 [/SUP], Vikram Padbidri[SUP] 5 [/SUP], Sampada Patwardhan[SUP] 6 [/SUP], Rohan Kate[SUP] 7 [/SUP]
Affiliations
Abstract
The main route of the transmission of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are through respiratory pathways and close contact of human-to-human. While information about other modes of transmission is comparatively less, some published literature supporting the likelihood of a fecal-oral mode of transmission has been accumulating. The diagnosis of SARS-COV-2 infected cases is based on the real-time reverse transcription-PCR (RT-PCR). The fecal excretion of SARS-COV-2 has been reported frequently, however, the role of fecal viral load with the severity of disease is not yet clear. Our study focused on the investigation of SARS-CoV-2 shedding in the fecal samples of patients with coronavirus disease 2019 (COVID-19). A total of 280 RT-PCR-positive patients were enrolled, among them 15.4% had gastrointestinal (GI) symptoms. It was shown that 62% of the patients were positive for SARS-CoV-2 RNA in fecal specimens. This positivity was not related to the presence of GI symptoms and the severity of disease. The next generation sequencing [NGS] of SARS-CoV-2 from fecal samples of patients was performed to analyze mutational variations. Findings from this study not only emphasized the potential presence of SARS-CoV-2 in feces, but also its continuing mutational changes and its possible role in fecal-oral transmission.
Keywords: COVID-19; NGS; SARS-CoV-2; fecal; real time RT-PCR.
. 2022 Mar 15;9:835168.
doi: 10.3389/fmed.2022.835168. eCollection 2022.
Prolonged Shedding of SARS-CoV-2 in Feces of COVID-19 Positive Patients: Trends in Genomic Variation in First and Second Wave
Mallika Lavania[SUP] 1 [/SUP], Madhuri S Joshi[SUP] 1 [/SUP], Sujata S Ranshing[SUP] 1 [/SUP], Varsha A Potdar[SUP] 2 [/SUP], Manohar Shinde[SUP] 1 [/SUP], Nutan Chavan[SUP] 1 [/SUP], Santosh M Jadhav[SUP] 3 [/SUP], Prasad Sarkale[SUP] 4 [/SUP], Sreelekshmy Mohandas[SUP] 4 [/SUP], Pradeep M Sawant[SUP] 1 [/SUP], Sanjaykumar Tikute[SUP] 1 [/SUP], Vikram Padbidri[SUP] 5 [/SUP], Sampada Patwardhan[SUP] 6 [/SUP], Rohan Kate[SUP] 7 [/SUP]
Affiliations
- PMID: 35372453
- PMCID: PMC8965355
- DOI: 10.3389/fmed.2022.835168
Abstract
The main route of the transmission of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are through respiratory pathways and close contact of human-to-human. While information about other modes of transmission is comparatively less, some published literature supporting the likelihood of a fecal-oral mode of transmission has been accumulating. The diagnosis of SARS-COV-2 infected cases is based on the real-time reverse transcription-PCR (RT-PCR). The fecal excretion of SARS-COV-2 has been reported frequently, however, the role of fecal viral load with the severity of disease is not yet clear. Our study focused on the investigation of SARS-CoV-2 shedding in the fecal samples of patients with coronavirus disease 2019 (COVID-19). A total of 280 RT-PCR-positive patients were enrolled, among them 15.4% had gastrointestinal (GI) symptoms. It was shown that 62% of the patients were positive for SARS-CoV-2 RNA in fecal specimens. This positivity was not related to the presence of GI symptoms and the severity of disease. The next generation sequencing [NGS] of SARS-CoV-2 from fecal samples of patients was performed to analyze mutational variations. Findings from this study not only emphasized the potential presence of SARS-CoV-2 in feces, but also its continuing mutational changes and its possible role in fecal-oral transmission.
Keywords: COVID-19; NGS; SARS-CoV-2; fecal; real time RT-PCR.