tetano
Editor, Senior Moderator
Front Immunol
. 2022 Jan 18;12:793197.
doi: 10.3389/fimmu.2021.793197. eCollection 2021.
SARS-CoV-2-Specific T Cell Responses Are Stronger in Children With Multisystem Inflammatory Syndrome Compared to Children With Uncomplicated SARS-CoV-2 Infection
Susan R Conway[SUP] 1 2 3 [/SUP], Christopher A Lazarski[SUP] 1 [/SUP], Naomi E Field[SUP] 1 [/SUP], Mariah Jensen-Wachspress[SUP] 1 [/SUP], Haili Lang[SUP] 1 [/SUP], Vaishnavi Kankate[SUP] 1 [/SUP], Jessica Durkee-Shock[SUP] 1 4 [/SUP], Hannah Kinoshita[SUP] 1 5 [/SUP], William Suslovic[SUP] 6 [/SUP], Kathleen Webber[SUP] 1 [/SUP], Karen Smith[SUP] 3 7 [/SUP], Jeffrey I Cohen[SUP] 4 [/SUP], Peter D Burbelo[SUP] 8 [/SUP], Anqing Zhang[SUP] 9 [/SUP], Stephen J Teach[SUP] 3 10 [/SUP], Trisha Ibeh[SUP] 10 [/SUP], Meghan Delaney[SUP] 3 6 [/SUP], Roberta L DeBiasi[SUP] 3 11 12 [/SUP], Michael D Keller[SUP] 1 13 14 [/SUP], Catherine M Bollard[SUP] 1 14 [/SUP]
Affiliations
Abstract
Background: Despite similar rates of infection, adults and children have markedly different morbidity and mortality related to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Compared to adults, children have infrequent severe manifestations of acute infection but are uniquely at risk for the rare and often severe Multisystem Inflammatory Syndrome in Children (MIS-C) following infection. We hypothesized that these differences in presentation are related to differences in the magnitude and/or antigen specificity of SARS-CoV-2-specific T cell (CST) responses between adults and children. We therefore set out to measure the CST response in convalescent adults versus children with and without MIS-C following SARS-CoV-2 infection.
Methods: CSTs were expanded from blood collected from convalescent children and adults post SARS-CoV-2 infection and evaluated by intracellular flow cytometry, surface markers, and cytokine production following stimulation with SARS-CoV-2-specific peptides. Presence of serum/plasma antibody to spike and nucleocapsid was measured using the luciferase immunoprecipitation systems (LIPS) assay.
Findings: Twenty-six of 27 MIS-C patients, 7 of 8 non-MIS-C convalescent children, and 13 of 14 adults were seropositive for spike and nucleocapsid antibody. CST responses in MIS-C patients were significantly higher than children with uncomplicated SARS-CoV-2 infection, but weaker than CST responses in convalescent adults.
Interpretation: Age-related differences in the magnitude of CST responses suggest differing post-infectious immunity to SARS-CoV-2 in children compared to adults post uncomplicated infection. Children with MIS-C have CST responses that are stronger than children with uncomplicated SARS-CoV-2 infection and weaker than convalescent adults, despite near uniform seropositivity.
Keywords: COVID-19; MIS-C; SARS-CoV-2; T cell; pediatric.
. 2022 Jan 18;12:793197.
doi: 10.3389/fimmu.2021.793197. eCollection 2021.
SARS-CoV-2-Specific T Cell Responses Are Stronger in Children With Multisystem Inflammatory Syndrome Compared to Children With Uncomplicated SARS-CoV-2 Infection
Susan R Conway[SUP] 1 2 3 [/SUP], Christopher A Lazarski[SUP] 1 [/SUP], Naomi E Field[SUP] 1 [/SUP], Mariah Jensen-Wachspress[SUP] 1 [/SUP], Haili Lang[SUP] 1 [/SUP], Vaishnavi Kankate[SUP] 1 [/SUP], Jessica Durkee-Shock[SUP] 1 4 [/SUP], Hannah Kinoshita[SUP] 1 5 [/SUP], William Suslovic[SUP] 6 [/SUP], Kathleen Webber[SUP] 1 [/SUP], Karen Smith[SUP] 3 7 [/SUP], Jeffrey I Cohen[SUP] 4 [/SUP], Peter D Burbelo[SUP] 8 [/SUP], Anqing Zhang[SUP] 9 [/SUP], Stephen J Teach[SUP] 3 10 [/SUP], Trisha Ibeh[SUP] 10 [/SUP], Meghan Delaney[SUP] 3 6 [/SUP], Roberta L DeBiasi[SUP] 3 11 12 [/SUP], Michael D Keller[SUP] 1 13 14 [/SUP], Catherine M Bollard[SUP] 1 14 [/SUP]
Affiliations
- PMID: 35116027
- PMCID: PMC8803660
- DOI: 10.3389/fimmu.2021.793197
Abstract
Background: Despite similar rates of infection, adults and children have markedly different morbidity and mortality related to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Compared to adults, children have infrequent severe manifestations of acute infection but are uniquely at risk for the rare and often severe Multisystem Inflammatory Syndrome in Children (MIS-C) following infection. We hypothesized that these differences in presentation are related to differences in the magnitude and/or antigen specificity of SARS-CoV-2-specific T cell (CST) responses between adults and children. We therefore set out to measure the CST response in convalescent adults versus children with and without MIS-C following SARS-CoV-2 infection.
Methods: CSTs were expanded from blood collected from convalescent children and adults post SARS-CoV-2 infection and evaluated by intracellular flow cytometry, surface markers, and cytokine production following stimulation with SARS-CoV-2-specific peptides. Presence of serum/plasma antibody to spike and nucleocapsid was measured using the luciferase immunoprecipitation systems (LIPS) assay.
Findings: Twenty-six of 27 MIS-C patients, 7 of 8 non-MIS-C convalescent children, and 13 of 14 adults were seropositive for spike and nucleocapsid antibody. CST responses in MIS-C patients were significantly higher than children with uncomplicated SARS-CoV-2 infection, but weaker than CST responses in convalescent adults.
Interpretation: Age-related differences in the magnitude of CST responses suggest differing post-infectious immunity to SARS-CoV-2 in children compared to adults post uncomplicated infection. Children with MIS-C have CST responses that are stronger than children with uncomplicated SARS-CoV-2 infection and weaker than convalescent adults, despite near uniform seropositivity.
Keywords: COVID-19; MIS-C; SARS-CoV-2; T cell; pediatric.