tetano
Editor, Senior Moderator
Front Immunol
. 2021 Sep 27;12:728021.
doi: 10.3389/fimmu.2021.728021. eCollection 2021.
SARS-CoV-2 mRNA Vaccines Elicit Different Responses in Immunologically Naïve and Pre-Immune Humans
David Forgacs[SUP] 1 [/SUP], Hyesun Jang[SUP] 1 [/SUP], Rodrigo B Abreu[SUP] 1 [/SUP], Hannah B Hanley[SUP] 1 [/SUP], Jasper L Gattiker[SUP] 1 [/SUP], Alexandria M Jefferson[SUP] 1 [/SUP], Ted M Ross[SUP] 1 2 [/SUP]
Affiliations
Abstract
As the COVID-19 pandemic continues, the authorization of vaccines for emergency use has been crucial in slowing down the rate of infection and transmission of the SARS-CoV-2 virus that causes COVID-19. In order to investigate the longitudinal serological responses to SARS-CoV-2 natural infection and vaccination, a large-scale, multi-year serosurveillance program entitled SPARTA (SARS SeroPrevalence and Respiratory Tract Assessment) was initiated at 4 locations in the U.S. The serological assay presented here measuring IgG binding to the SARS-CoV-2 receptor binding domain (RBD) detected antibodies elicited by SARS-CoV-2 infection or vaccination with a 95.5% sensitivity and a 95.9% specificity. We used this assay to screen more than 3100 participants and selected 20 previously infected pre-immune and 32 immunologically naïve participants to analyze their antibody binding to RBD and viral neutralization (VN) responses following vaccination with two doses of either the Pfizer-BioNTech BNT162b2 or the Moderna mRNA-1273 vaccine. Vaccination not only elicited a more robust immune reaction than natural infection, but the level of neutralizing and anti-RBD antibody binding after vaccination is also significantly higher in pre-immune participants compared to immunologically naïve participants (p<0.0033). Furthermore, the administration of the second vaccination did not further increase the neutralizing or binding antibody levels in pre-immune participants (p=0.69). However, ~46% of the immunologically naïve participants required both vaccinations to seroconvert.
Keywords: SARS-CoV-2; antibody response; coronavirus; immunization; neutralization; pre-immune; vaccination.
. 2021 Sep 27;12:728021.
doi: 10.3389/fimmu.2021.728021. eCollection 2021.
SARS-CoV-2 mRNA Vaccines Elicit Different Responses in Immunologically Naïve and Pre-Immune Humans
David Forgacs[SUP] 1 [/SUP], Hyesun Jang[SUP] 1 [/SUP], Rodrigo B Abreu[SUP] 1 [/SUP], Hannah B Hanley[SUP] 1 [/SUP], Jasper L Gattiker[SUP] 1 [/SUP], Alexandria M Jefferson[SUP] 1 [/SUP], Ted M Ross[SUP] 1 2 [/SUP]
Affiliations
- PMID: 34646267
- PMCID: PMC8502960
- DOI: 10.3389/fimmu.2021.728021
Abstract
As the COVID-19 pandemic continues, the authorization of vaccines for emergency use has been crucial in slowing down the rate of infection and transmission of the SARS-CoV-2 virus that causes COVID-19. In order to investigate the longitudinal serological responses to SARS-CoV-2 natural infection and vaccination, a large-scale, multi-year serosurveillance program entitled SPARTA (SARS SeroPrevalence and Respiratory Tract Assessment) was initiated at 4 locations in the U.S. The serological assay presented here measuring IgG binding to the SARS-CoV-2 receptor binding domain (RBD) detected antibodies elicited by SARS-CoV-2 infection or vaccination with a 95.5% sensitivity and a 95.9% specificity. We used this assay to screen more than 3100 participants and selected 20 previously infected pre-immune and 32 immunologically naïve participants to analyze their antibody binding to RBD and viral neutralization (VN) responses following vaccination with two doses of either the Pfizer-BioNTech BNT162b2 or the Moderna mRNA-1273 vaccine. Vaccination not only elicited a more robust immune reaction than natural infection, but the level of neutralizing and anti-RBD antibody binding after vaccination is also significantly higher in pre-immune participants compared to immunologically naïve participants (p<0.0033). Furthermore, the administration of the second vaccination did not further increase the neutralizing or binding antibody levels in pre-immune participants (p=0.69). However, ~46% of the immunologically naïve participants required both vaccinations to seroconvert.
Keywords: SARS-CoV-2; antibody response; coronavirus; immunization; neutralization; pre-immune; vaccination.