tetano
Editor, Senior Moderator
Front Immunol
. 2022 Oct 19;13:1042406.
doi: 10.3389/fimmu.2022.1042406. eCollection 2022.
Pre-existing humoral immunity to low pathogenic human coronaviruses exhibits limited cross-reactive antibodies response against SARS-CoV-2 in children
Nina Li[SUP] 1 2 [/SUP], XueYun Li[SUP] 1 2 [/SUP], Jiani Wu[SUP] 1 2 [/SUP], Shengze Zhang[SUP] 1 2 [/SUP], Lin Zhu[SUP] 1 2 [/SUP], Qiqi Chen[SUP] 1 2 [/SUP], Ying Fan[SUP] 1 2 [/SUP], Zhengyu Wu[SUP] 1 2 [/SUP], Sidian Xie[SUP] 1 2 [/SUP], Qi Chen[SUP] 1 2 [/SUP], Ning Wang[SUP] 3 [/SUP], Nan Wu[SUP] 4 [/SUP], Chuming Luo[SUP] 1 2 [/SUP], Yuelong Shu[SUP] 1 2 5 6 [/SUP], Huanle Luo[SUP] 1 2 6 [/SUP]
Affiliations
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection causes asymptomatic or mild symptoms, even rare hospitalization in children. A major concern is whether the pre-existing antibodies induced by low pathogenic human coronaviruses (LPH-CoVs) in children can cross-react with SARS-CoV-2. To address this unresolved question, we analyzed the pre-existing spike (S)-specific immunoglobin (Ig) G antibodies against LPH-CoVs and the cross-reactive antibodies against SARS-CoV-2 in 658 serum samples collected from children prior to SARS-CoV-2 outbreak. We found that the seroprevalence of these four LPH-CoVs reached 75.84%, and about 24.64% of the seropositive samples had cross-reactive IgG antibodies against the nucleocapsid, S, and receptor binding domain antigens of SARS-CoV-2. Additionally, the re-infections with different LPH-CoVs occurred frequently in children and tended to increase the cross-reactive antibodies against SARS-CoV-2. From the forty-nine serum samples with cross-reactive anti-S IgG antibodies against SARS-CoV-2, we found that seven samples with a median age of 1.4 years old had detected neutralizing activity for the wild-type or mutant SARS-CoV-2 S pseudotypes. Interestingly, all of the seven samples contained anti-S IgG antibodies against HCoV-OC43. Together, these data suggest that children's pre-existing antibodies to LPH-CoVs have limited cross-reactive neutralizing antibodies against SRAS-CoV-2.
Keywords: SARS-CoV-2; antigen specific antibody; cross-reactive antibody; low pathogenic human coronaviruses; neutralizing activity.
. 2022 Oct 19;13:1042406.
doi: 10.3389/fimmu.2022.1042406. eCollection 2022.
Pre-existing humoral immunity to low pathogenic human coronaviruses exhibits limited cross-reactive antibodies response against SARS-CoV-2 in children
Nina Li[SUP] 1 2 [/SUP], XueYun Li[SUP] 1 2 [/SUP], Jiani Wu[SUP] 1 2 [/SUP], Shengze Zhang[SUP] 1 2 [/SUP], Lin Zhu[SUP] 1 2 [/SUP], Qiqi Chen[SUP] 1 2 [/SUP], Ying Fan[SUP] 1 2 [/SUP], Zhengyu Wu[SUP] 1 2 [/SUP], Sidian Xie[SUP] 1 2 [/SUP], Qi Chen[SUP] 1 2 [/SUP], Ning Wang[SUP] 3 [/SUP], Nan Wu[SUP] 4 [/SUP], Chuming Luo[SUP] 1 2 [/SUP], Yuelong Shu[SUP] 1 2 5 6 [/SUP], Huanle Luo[SUP] 1 2 6 [/SUP]
Affiliations
- PMID: 36341358
- PMCID: PMC9626651
- DOI: 10.3389/fimmu.2022.1042406
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection causes asymptomatic or mild symptoms, even rare hospitalization in children. A major concern is whether the pre-existing antibodies induced by low pathogenic human coronaviruses (LPH-CoVs) in children can cross-react with SARS-CoV-2. To address this unresolved question, we analyzed the pre-existing spike (S)-specific immunoglobin (Ig) G antibodies against LPH-CoVs and the cross-reactive antibodies against SARS-CoV-2 in 658 serum samples collected from children prior to SARS-CoV-2 outbreak. We found that the seroprevalence of these four LPH-CoVs reached 75.84%, and about 24.64% of the seropositive samples had cross-reactive IgG antibodies against the nucleocapsid, S, and receptor binding domain antigens of SARS-CoV-2. Additionally, the re-infections with different LPH-CoVs occurred frequently in children and tended to increase the cross-reactive antibodies against SARS-CoV-2. From the forty-nine serum samples with cross-reactive anti-S IgG antibodies against SARS-CoV-2, we found that seven samples with a median age of 1.4 years old had detected neutralizing activity for the wild-type or mutant SARS-CoV-2 S pseudotypes. Interestingly, all of the seven samples contained anti-S IgG antibodies against HCoV-OC43. Together, these data suggest that children's pre-existing antibodies to LPH-CoVs have limited cross-reactive neutralizing antibodies against SRAS-CoV-2.
Keywords: SARS-CoV-2; antigen specific antibody; cross-reactive antibody; low pathogenic human coronaviruses; neutralizing activity.