tetano
Editor, Senior Moderator
Front Immunol
. 2023 Aug 30;14:1243164.
doi: 10.3389/fimmu.2023.1243164. eCollection 2023. Pre-existing CD4 T cell help boosts antibody responses but has limited impact on germinal center, antigen-specific B cell frequencies after influenza infection
Danica F Besavilla[SUP] 1 [/SUP], Laura Reusch[SUP] 1 [/SUP], Josue Enriquez[SUP] 1 [/SUP], Karin Schön[SUP] 1 [/SUP], Davide Angeletti[SUP] 1 2 [/SUP]
Affiliations
The influenza virus is a persistent burden on global health, with seasonal vaccines providing incomplete protection. CD4[SUP]+[/SUP] T cells help shape B cell and antibody responses; however, the selectivity of help and the effect on various antigen-specific B cell populations have not been fully elucidated. Here, we studied the specificity, selectivity, and influence of nucleoprotein (NP) CD4[SUP]+[/SUP] T cells on the magnitude and quality of hemagglutinin (HA) and NP-specific B cells and antibody responses. We identified immunodominant peptides and showed that peptide immunization was sufficient to induce CD4[SUP]+[/SUP] cells with Th1 and Tfh phenotypes. Surprisingly, while preexisting CD4[SUP]+[/SUP] T cells enhanced the influx of total germinal center (GC) B cells in the mediastinal lymph node after infection, this was not reflected by an increase in the frequency of antigen-specific cells within the GC. Furthermore, we demonstrated that NP-specific help was able to accelerate the kinetics and magnitude of the Ab response for NP but not for HA. Overall, our results showed that pre-existing CD4[SUP]+[/SUP] T cells provide strong cognate help during immunization or infection to enhance Ab production but not antigen-specific GC or memory B cells.
Keywords: B cells; CD4+ T cells; antibodies; germinal center; influenza.
. 2023 Aug 30;14:1243164.
doi: 10.3389/fimmu.2023.1243164. eCollection 2023. Pre-existing CD4 T cell help boosts antibody responses but has limited impact on germinal center, antigen-specific B cell frequencies after influenza infection
Danica F Besavilla[SUP] 1 [/SUP], Laura Reusch[SUP] 1 [/SUP], Josue Enriquez[SUP] 1 [/SUP], Karin Schön[SUP] 1 [/SUP], Davide Angeletti[SUP] 1 2 [/SUP]
Affiliations
- PMID: 37711622
- PMCID: PMC10499173
- DOI: 10.3389/fimmu.2023.1243164
The influenza virus is a persistent burden on global health, with seasonal vaccines providing incomplete protection. CD4[SUP]+[/SUP] T cells help shape B cell and antibody responses; however, the selectivity of help and the effect on various antigen-specific B cell populations have not been fully elucidated. Here, we studied the specificity, selectivity, and influence of nucleoprotein (NP) CD4[SUP]+[/SUP] T cells on the magnitude and quality of hemagglutinin (HA) and NP-specific B cells and antibody responses. We identified immunodominant peptides and showed that peptide immunization was sufficient to induce CD4[SUP]+[/SUP] cells with Th1 and Tfh phenotypes. Surprisingly, while preexisting CD4[SUP]+[/SUP] T cells enhanced the influx of total germinal center (GC) B cells in the mediastinal lymph node after infection, this was not reflected by an increase in the frequency of antigen-specific cells within the GC. Furthermore, we demonstrated that NP-specific help was able to accelerate the kinetics and magnitude of the Ab response for NP but not for HA. Overall, our results showed that pre-existing CD4[SUP]+[/SUP] T cells provide strong cognate help during immunization or infection to enhance Ab production but not antigen-specific GC or memory B cells.
Keywords: B cells; CD4+ T cells; antibodies; germinal center; influenza.