tetano
Editor, Senior Moderator
Front Immunol
. 2022 Sep 20;13:952650.
doi: 10.3389/fimmu.2022.952650. eCollection 2022.
Potential of conserved antigenic sites in development of universal SARS-like coronavirus vaccines
Siling Wang[SUP] 1 [/SUP], Dinghui Wu[SUP] 2 [/SUP], Hualong Xiong[SUP] 1 [/SUP], Juan Wang[SUP] 1 [/SUP], Zimin Tang[SUP] 1 [/SUP], Zihao Chen[SUP] 1 [/SUP], Yizhen Wang[SUP] 1 [/SUP], Yali Zhang[SUP] 1 [/SUP], Dong Ying[SUP] 1 [/SUP], Xue Lin[SUP] 1 [/SUP], Chang Liu[SUP] 1 [/SUP], Shaoqi Guo[SUP] 1 [/SUP], Weikun Tian[SUP] 1 [/SUP], Yajie Lin[SUP] 1 [/SUP], Xiaoping Zhang[SUP] 1 [/SUP], Quan Yuan[SUP] 1 [/SUP], Hai Yu[SUP] 1 [/SUP], Tianying Zhang[SUP] 1 [/SUP], Zizheng Zheng[SUP] 1 [/SUP], Ningshao Xia[SUP] 1 [/SUP]
Affiliations
Abstract
Given pandemic risks of zoonotic SARS-CoV-2 variants and other SARS-like coronaviruses in the future, it is valuable to perform studies on conserved antigenic sites to design universal SARS-like coronavirus vaccines. By using antibodies obtained from convalescent COVID-19 patients, we succeeded in functional comparison of conserved antigenic sites at multiple aspects with each other, and even with SARS-CoV-2 unique antigenic sites, which promotes the cognition of process of humoral immune response to the conserved antigenic sites. The conserved antigenic sites between SARS-CoV-2 and SARS-CoV can effectively induce affinity maturation of cross-binding antibodies, finally resulting in broadly neutralizing antibodies against multiple variants of concern, which provides an important basis for universal vaccine design, however they are subdominant, putatively due to their lower accessibility relative to SARS-CoV-2 unique antigenic sites. Furthermore, we preliminarily design RBDs to improve the immunogenicity of these conserved antigenic sites. Our study focusing on conserved antigenic sites provides insights for promoting the development of universal SARS-like coronavirus vaccines, thereby enhancing our pandemic preparedness.
Keywords: COVID-19; SARS-CoV-2; conserved antigenic sites; convalescent individual; cross-neutralizing antibodies; universal vaccine.
. 2022 Sep 20;13:952650.
doi: 10.3389/fimmu.2022.952650. eCollection 2022.
Potential of conserved antigenic sites in development of universal SARS-like coronavirus vaccines
Siling Wang[SUP] 1 [/SUP], Dinghui Wu[SUP] 2 [/SUP], Hualong Xiong[SUP] 1 [/SUP], Juan Wang[SUP] 1 [/SUP], Zimin Tang[SUP] 1 [/SUP], Zihao Chen[SUP] 1 [/SUP], Yizhen Wang[SUP] 1 [/SUP], Yali Zhang[SUP] 1 [/SUP], Dong Ying[SUP] 1 [/SUP], Xue Lin[SUP] 1 [/SUP], Chang Liu[SUP] 1 [/SUP], Shaoqi Guo[SUP] 1 [/SUP], Weikun Tian[SUP] 1 [/SUP], Yajie Lin[SUP] 1 [/SUP], Xiaoping Zhang[SUP] 1 [/SUP], Quan Yuan[SUP] 1 [/SUP], Hai Yu[SUP] 1 [/SUP], Tianying Zhang[SUP] 1 [/SUP], Zizheng Zheng[SUP] 1 [/SUP], Ningshao Xia[SUP] 1 [/SUP]
Affiliations
- PMID: 36203593
- PMCID: PMC9530325
- DOI: 10.3389/fimmu.2022.952650
Abstract
Given pandemic risks of zoonotic SARS-CoV-2 variants and other SARS-like coronaviruses in the future, it is valuable to perform studies on conserved antigenic sites to design universal SARS-like coronavirus vaccines. By using antibodies obtained from convalescent COVID-19 patients, we succeeded in functional comparison of conserved antigenic sites at multiple aspects with each other, and even with SARS-CoV-2 unique antigenic sites, which promotes the cognition of process of humoral immune response to the conserved antigenic sites. The conserved antigenic sites between SARS-CoV-2 and SARS-CoV can effectively induce affinity maturation of cross-binding antibodies, finally resulting in broadly neutralizing antibodies against multiple variants of concern, which provides an important basis for universal vaccine design, however they are subdominant, putatively due to their lower accessibility relative to SARS-CoV-2 unique antigenic sites. Furthermore, we preliminarily design RBDs to improve the immunogenicity of these conserved antigenic sites. Our study focusing on conserved antigenic sites provides insights for promoting the development of universal SARS-like coronavirus vaccines, thereby enhancing our pandemic preparedness.
Keywords: COVID-19; SARS-CoV-2; conserved antigenic sites; convalescent individual; cross-neutralizing antibodies; universal vaccine.