tetano
Editor, Senior Moderator
Front Immunol
. 2022 Apr 12;13:817345.
doi: 10.3389/fimmu.2022.817345. eCollection 2022.
Persistence of SARS-CoV-2 Antibodies in Vaccinated Health Care Workers Analyzed by Coronavirus Antigen Microarray
Sina Hosseinian[SUP] 1 [/SUP], Kathleen Powers[SUP] 1 [/SUP], Milind Vasudev[SUP] 1 [/SUP], Anton M Palma[SUP] 2 [/SUP], Rafael de Assis[SUP] 3 [/SUP], Aarti Jain[SUP] 3 [/SUP], Peter Horvath[SUP] 2 [/SUP], Paramveer S Birring[SUP] 1 [/SUP], Rana Andary[SUP] 1 [/SUP], Connie Au[SUP] 1 [/SUP], Brandon Chin[SUP] 1 [/SUP], Ghali Khalil[SUP] 1 [/SUP], Jenny Ventura[SUP] 1 [/SUP], Madeleine K Luu[SUP] 4 [/SUP], Cesar Figueroa[SUP] 5 [/SUP], Joshua M Obiero[SUP] 3 [/SUP], Emily Silzel[SUP] 3 [/SUP], Rie Nakajima[SUP] 3 [/SUP], William Thomas Gombrich[SUP] 4 [/SUP], Algis Jasinskas[SUP] 3 [/SUP], Frank Zaldivar[SUP] 2 6 [/SUP], Sebastian Schubl[SUP] 1 5 [/SUP], Philip L Felgner[SUP] 3 [/SUP], Saahir Khan[SUP] 7 [/SUP], Specimen Collection Group
Affiliations
Abstract
Recent studies provide conflicting evidence on the persistence of SARS-CoV-2 immunity induced by mRNA vaccines. Here, we aim to quantify the persistence of humoral immunity following vaccination using a coronavirus antigen microarray that includes 10 SARS-CoV-2 antigens. In a prospective longitudinal cohort of 240 healthcare workers, composite SARS-CoV-2 IgG antibody levels did not wane significantly over a 6-month study period. In the subset of the study population previously exposed to SARS-CoV-2 based on seropositivity for nucleocapsid antibodies, higher composite anti-spike IgG levels were measured before the vaccine but no significant difference from unexposed individuals was observed at 6 months. Age, vaccine type, or worker role did not significantly impact composite IgG levels, although non-significant trends towards lower antibody levels in older participants and higher antibody levels with Moderna vaccine were observed at 6 months. A small subset of our cohort were classified as having waning antibody titers at 6 months, and these individuals were less likely to work in patient care roles and more likely to have prior exposure to SARS-CoV-2.
Keywords: SARS-CoV-2; antibodies; healthcare workers; mRNA; microarray; serology; vaccine.
. 2022 Apr 12;13:817345.
doi: 10.3389/fimmu.2022.817345. eCollection 2022.
Persistence of SARS-CoV-2 Antibodies in Vaccinated Health Care Workers Analyzed by Coronavirus Antigen Microarray
Sina Hosseinian[SUP] 1 [/SUP], Kathleen Powers[SUP] 1 [/SUP], Milind Vasudev[SUP] 1 [/SUP], Anton M Palma[SUP] 2 [/SUP], Rafael de Assis[SUP] 3 [/SUP], Aarti Jain[SUP] 3 [/SUP], Peter Horvath[SUP] 2 [/SUP], Paramveer S Birring[SUP] 1 [/SUP], Rana Andary[SUP] 1 [/SUP], Connie Au[SUP] 1 [/SUP], Brandon Chin[SUP] 1 [/SUP], Ghali Khalil[SUP] 1 [/SUP], Jenny Ventura[SUP] 1 [/SUP], Madeleine K Luu[SUP] 4 [/SUP], Cesar Figueroa[SUP] 5 [/SUP], Joshua M Obiero[SUP] 3 [/SUP], Emily Silzel[SUP] 3 [/SUP], Rie Nakajima[SUP] 3 [/SUP], William Thomas Gombrich[SUP] 4 [/SUP], Algis Jasinskas[SUP] 3 [/SUP], Frank Zaldivar[SUP] 2 6 [/SUP], Sebastian Schubl[SUP] 1 5 [/SUP], Philip L Felgner[SUP] 3 [/SUP], Saahir Khan[SUP] 7 [/SUP], Specimen Collection Group
Affiliations
- PMID: 35493473
- PMCID: PMC9040070
- DOI: 10.3389/fimmu.2022.817345
Abstract
Recent studies provide conflicting evidence on the persistence of SARS-CoV-2 immunity induced by mRNA vaccines. Here, we aim to quantify the persistence of humoral immunity following vaccination using a coronavirus antigen microarray that includes 10 SARS-CoV-2 antigens. In a prospective longitudinal cohort of 240 healthcare workers, composite SARS-CoV-2 IgG antibody levels did not wane significantly over a 6-month study period. In the subset of the study population previously exposed to SARS-CoV-2 based on seropositivity for nucleocapsid antibodies, higher composite anti-spike IgG levels were measured before the vaccine but no significant difference from unexposed individuals was observed at 6 months. Age, vaccine type, or worker role did not significantly impact composite IgG levels, although non-significant trends towards lower antibody levels in older participants and higher antibody levels with Moderna vaccine were observed at 6 months. A small subset of our cohort were classified as having waning antibody titers at 6 months, and these individuals were less likely to work in patient care roles and more likely to have prior exposure to SARS-CoV-2.
Keywords: SARS-CoV-2; antibodies; healthcare workers; mRNA; microarray; serology; vaccine.