tetano
Editor, Senior Moderator
Front Immunol
. 2021 Dec 9;12:779026.
doi: 10.3389/fimmu.2021.779026. eCollection 2021.
Evidence of SARS-CoV-2-Specific T-Cell-Mediated Myocarditis in a MIS-A Case
Kevin M Vannella[SUP] 1 2 [/SUP], Cihan Oguz[SUP] 3 4 [/SUP], Sydney R Stein[SUP] 1 2 [/SUP], Stefania Pittaluga[SUP] 5 [/SUP], Esra Dikoglu[SUP] 5 [/SUP], Arjun Kanwal[SUP] 6 [/SUP], Sabrina C Ramelli[SUP] 7 [/SUP], Thomas Briese[SUP] 8 [/SUP], Ling Su[SUP] 9 [/SUP], Xiaolin Wu[SUP] 9 [/SUP], Marcos J Ramos-Benitez[SUP] 1 2 10 [/SUP], Luis J Perez-Valencia[SUP] 1 2 [/SUP], Ashley Babyak[SUP] 1 2 [/SUP], Nu Ri Cha[SUP] 11 [/SUP], Joon-Yong Chung[SUP] 5 [/SUP], Kris Ylaya[SUP] 5 [/SUP], Ronson J Madathil[SUP] 12 [/SUP], Kapil K Saharia[SUP] 13 [/SUP], Thomas M Scalea[SUP] 14 [/SUP], Quincy K Tran[SUP] 15 [/SUP], Daniel L Herr[SUP] 16 [/SUP], David E Kleiner[SUP] 5 [/SUP], Stephen M Hewitt[SUP] 5 [/SUP], Luigi D Notarangelo[SUP] 17 [/SUP], Alison Grazioli[SUP] 18 [/SUP], Daniel S Chertow[SUP] 1 2 [/SUP]
Affiliations
Abstract
A 26-year-old otherwise healthy man died of fulminant myocarditis. Nasopharyngeal specimens collected premortem tested negative for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Histopathological evaluation of the heart showed myocardial necrosis surrounded by cytotoxic T-cells and tissue-repair macrophages. Myocardial T-cell receptor (TCR) sequencing revealed hyper-dominant clones with highly similar sequences to TCRs that are specific for SARS-CoV-2 epitopes. SARS-CoV-2 RNA was detected in the gut, supporting a diagnosis of multisystem inflammatory syndrome in adults (MIS-A). Molecular targets of MIS-associated inflammation are not known. Our data indicate that SARS-CoV-2 antigens selected high-frequency T-cell clones that mediated fatal myocarditis.
Keywords: CDR3 sequences; MIS-A; SARS-CoV-2; SARS-CoV-2 epitopes; T cell receptor (TCR); cd-hit; myocarditis.
. 2021 Dec 9;12:779026.
doi: 10.3389/fimmu.2021.779026. eCollection 2021.
Evidence of SARS-CoV-2-Specific T-Cell-Mediated Myocarditis in a MIS-A Case
Kevin M Vannella[SUP] 1 2 [/SUP], Cihan Oguz[SUP] 3 4 [/SUP], Sydney R Stein[SUP] 1 2 [/SUP], Stefania Pittaluga[SUP] 5 [/SUP], Esra Dikoglu[SUP] 5 [/SUP], Arjun Kanwal[SUP] 6 [/SUP], Sabrina C Ramelli[SUP] 7 [/SUP], Thomas Briese[SUP] 8 [/SUP], Ling Su[SUP] 9 [/SUP], Xiaolin Wu[SUP] 9 [/SUP], Marcos J Ramos-Benitez[SUP] 1 2 10 [/SUP], Luis J Perez-Valencia[SUP] 1 2 [/SUP], Ashley Babyak[SUP] 1 2 [/SUP], Nu Ri Cha[SUP] 11 [/SUP], Joon-Yong Chung[SUP] 5 [/SUP], Kris Ylaya[SUP] 5 [/SUP], Ronson J Madathil[SUP] 12 [/SUP], Kapil K Saharia[SUP] 13 [/SUP], Thomas M Scalea[SUP] 14 [/SUP], Quincy K Tran[SUP] 15 [/SUP], Daniel L Herr[SUP] 16 [/SUP], David E Kleiner[SUP] 5 [/SUP], Stephen M Hewitt[SUP] 5 [/SUP], Luigi D Notarangelo[SUP] 17 [/SUP], Alison Grazioli[SUP] 18 [/SUP], Daniel S Chertow[SUP] 1 2 [/SUP]
Affiliations
- PMID: 34956207
- PMCID: PMC8695925
- DOI: 10.3389/fimmu.2021.779026
Abstract
A 26-year-old otherwise healthy man died of fulminant myocarditis. Nasopharyngeal specimens collected premortem tested negative for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Histopathological evaluation of the heart showed myocardial necrosis surrounded by cytotoxic T-cells and tissue-repair macrophages. Myocardial T-cell receptor (TCR) sequencing revealed hyper-dominant clones with highly similar sequences to TCRs that are specific for SARS-CoV-2 epitopes. SARS-CoV-2 RNA was detected in the gut, supporting a diagnosis of multisystem inflammatory syndrome in adults (MIS-A). Molecular targets of MIS-associated inflammation are not known. Our data indicate that SARS-CoV-2 antigens selected high-frequency T-cell clones that mediated fatal myocarditis.
Keywords: CDR3 sequences; MIS-A; SARS-CoV-2; SARS-CoV-2 epitopes; T cell receptor (TCR); cd-hit; myocarditis.