tetano
Editor, Senior Moderator
Front Immunol
. 2022 Jun 15;13:915034.
doi: 10.3389/fimmu.2022.915034. eCollection 2022.
Cross-Reactivity of IgG Antibodies and Virus Neutralization in mRNA-Vaccinated People Against Wild-Type SARS-CoV-2 and the Five Most Common SARS-CoV-2 Variants of Concern
Mandy Schwarze[SUP] 1 2 [/SUP], Andor Krizsan[SUP] 1 2 [/SUP], Alexandra Brakel[SUP] 1 2 [/SUP], Fabian Pohl[SUP] 1 2 [/SUP], Daniela Volke[SUP] 1 2 [/SUP], Ralf Hoffmann[SUP] 1 2 [/SUP]
Affiliations
Abstract
The rapid development, approval, and production of vaccines against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in less than 1 year after the first reports of a new infectious disease was a real game changer, providing 80%-90% efficacy in preventing severe etiopathologies of the coronavirus disease 2019 (COVID-19). These vaccines induce an immune response against the SARS-CoV-2 spike (S) protein located on the surface of the virus particle. Antibodies (Abs) recognizing the S-protein can inhibit binding of the virus via the S-protein to the angiotensin-converting enzyme-2 (ACE-2) receptor expressed on different human cells, especially when these Abs bind to the interaction site, the so-called receptor-binding domain (RBD). We have expressed the RBDs of wild-type SARS-CoV-2 and five variants of concern (VOCs) to test the immune response in people before vaccination with mRNA vaccines BNT162b2 and mRNA-1273 and after up to three vaccinations using in-house ELISA and inhibition assays. The methods of both assays are provided. Both vaccines initiated similarly high IgG titers after two vaccinations against the wild-type and even two VOC-RBDs (alpha and delta) and strongly inhibited the corresponding RBD-ACE-2 binding. The IgG titers and inhibition of ACE-2 binding were lower for beta and gamma RBDs and much lower for omicron RBD. The third vaccination after 6 months strongly increased both the IgG titers and the neutralizing effect against all variants, especially for omicron, leading to 63% ± 13% neutralization potential. Importantly, neutralization linearly increased with the IgG titers.
Keywords: BNT162b2; ELISA; SARS-CoV-2; angiotensin-converting enzyme-2 (ACE-2); mRNA-1273; receptor-binding domain (RBD).
. 2022 Jun 15;13:915034.
doi: 10.3389/fimmu.2022.915034. eCollection 2022.
Cross-Reactivity of IgG Antibodies and Virus Neutralization in mRNA-Vaccinated People Against Wild-Type SARS-CoV-2 and the Five Most Common SARS-CoV-2 Variants of Concern
Mandy Schwarze[SUP] 1 2 [/SUP], Andor Krizsan[SUP] 1 2 [/SUP], Alexandra Brakel[SUP] 1 2 [/SUP], Fabian Pohl[SUP] 1 2 [/SUP], Daniela Volke[SUP] 1 2 [/SUP], Ralf Hoffmann[SUP] 1 2 [/SUP]
Affiliations
- PMID: 35784285
- PMCID: PMC9242094
- DOI: 10.3389/fimmu.2022.915034
Abstract
The rapid development, approval, and production of vaccines against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in less than 1 year after the first reports of a new infectious disease was a real game changer, providing 80%-90% efficacy in preventing severe etiopathologies of the coronavirus disease 2019 (COVID-19). These vaccines induce an immune response against the SARS-CoV-2 spike (S) protein located on the surface of the virus particle. Antibodies (Abs) recognizing the S-protein can inhibit binding of the virus via the S-protein to the angiotensin-converting enzyme-2 (ACE-2) receptor expressed on different human cells, especially when these Abs bind to the interaction site, the so-called receptor-binding domain (RBD). We have expressed the RBDs of wild-type SARS-CoV-2 and five variants of concern (VOCs) to test the immune response in people before vaccination with mRNA vaccines BNT162b2 and mRNA-1273 and after up to three vaccinations using in-house ELISA and inhibition assays. The methods of both assays are provided. Both vaccines initiated similarly high IgG titers after two vaccinations against the wild-type and even two VOC-RBDs (alpha and delta) and strongly inhibited the corresponding RBD-ACE-2 binding. The IgG titers and inhibition of ACE-2 binding were lower for beta and gamma RBDs and much lower for omicron RBD. The third vaccination after 6 months strongly increased both the IgG titers and the neutralizing effect against all variants, especially for omicron, leading to 63% ± 13% neutralization potential. Importantly, neutralization linearly increased with the IgG titers.
Keywords: BNT162b2; ELISA; SARS-CoV-2; angiotensin-converting enzyme-2 (ACE-2); mRNA-1273; receptor-binding domain (RBD).