tetano
Editor, Senior Moderator
Front Immunol
. 2024 Jul 19:15:1442160.
doi: 10.3389/fimmu.2024.1442160. eCollection 2024. A potent, broadly neutralizing human monoclonal antibody that efficiently protects hACE2-transgenic mice from infection with the Wuhan, BA.5, and XBB.1.5 SARS-CoV-2 variants
Sergey V Guselnikov[SUP] #[/SUP][SUP] 1 [/SUP], Konstantin O Baranov[SUP] #[/SUP][SUP] 1 [/SUP], Sergey V Kulemzin[SUP] 1 [/SUP], Tatyana N Belovezhets[SUP] 1 [/SUP], Anton N Chikaev[SUP] 1 [/SUP], Svetlana V Murasheva[SUP] 1 [/SUP], Olga Y Volkova[SUP] 1 [/SUP], Ludmila V Mechetina[SUP] 1 [/SUP], Alexander M Najakshin[SUP] 1 [/SUP], Nikolai A Chikaev[SUP] 1 [/SUP], Pavel P Solodkov[SUP] 1 [/SUP], Maria V Sergeeva[SUP] 2 [/SUP], Alexander V Smirnov[SUP] 3 [/SUP], Irina A Serova[SUP] 3 [/SUP], Oleg L Serov[SUP] 3 [/SUP], Alexander G Markhaev[SUP] 4 [/SUP], Yulia V Kononova[SUP] 4 [/SUP], Alexander Y Alekseev[SUP] 4 5 [/SUP], Marina A Gulyaeva[SUP] 4 5 [/SUP], Daria M Danilenko[SUP] 6 [/SUP], Nariman R Battulin[SUP] 3 5 [/SUP], Alexander M Shestopalov[SUP] 4 5 [/SUP], Alexander V Taranin[SUP] 1 [/SUP]
Affiliations
The COVID-19 pandemic has uncovered the high genetic variability of the SARS-CoV-2 virus and its ability to evade the immune responses that were induced by earlier viral variants. Only a few monoclonal antibodies that have been reported to date are capable of neutralizing a broad spectrum of SARS-CoV-2 variants. Here, we report the isolation of a new broadly neutralizing human monoclonal antibody, iC1. The antibody was identified through sorting the SARS-CoV-1 RBD-stained individual B cells that were isolated from the blood of a vaccinated donor following a breakthrough infection. In vitro, iC1 potently neutralizes pseudoviruses expressing a wide range of SARS-CoV-2 Spike variants, including those of the XBB sublineage. In an hACE2-transgenic mouse model, iC1 provided effective protection against the Wuhan strain of the virus as well as the BA.5 and XBB.1.5 variants. Therefore, iC1 can be considered as a potential component of the broadly neutralizing antibody cocktails resisting the SARS-CoV-2 mutation escape.
Keywords: COVID-19; Omicron; SARS-CoV-1; iC1 antibody; in vivo protection.
. 2024 Jul 19:15:1442160.
doi: 10.3389/fimmu.2024.1442160. eCollection 2024. A potent, broadly neutralizing human monoclonal antibody that efficiently protects hACE2-transgenic mice from infection with the Wuhan, BA.5, and XBB.1.5 SARS-CoV-2 variants
Sergey V Guselnikov[SUP] #[/SUP][SUP] 1 [/SUP], Konstantin O Baranov[SUP] #[/SUP][SUP] 1 [/SUP], Sergey V Kulemzin[SUP] 1 [/SUP], Tatyana N Belovezhets[SUP] 1 [/SUP], Anton N Chikaev[SUP] 1 [/SUP], Svetlana V Murasheva[SUP] 1 [/SUP], Olga Y Volkova[SUP] 1 [/SUP], Ludmila V Mechetina[SUP] 1 [/SUP], Alexander M Najakshin[SUP] 1 [/SUP], Nikolai A Chikaev[SUP] 1 [/SUP], Pavel P Solodkov[SUP] 1 [/SUP], Maria V Sergeeva[SUP] 2 [/SUP], Alexander V Smirnov[SUP] 3 [/SUP], Irina A Serova[SUP] 3 [/SUP], Oleg L Serov[SUP] 3 [/SUP], Alexander G Markhaev[SUP] 4 [/SUP], Yulia V Kononova[SUP] 4 [/SUP], Alexander Y Alekseev[SUP] 4 5 [/SUP], Marina A Gulyaeva[SUP] 4 5 [/SUP], Daria M Danilenko[SUP] 6 [/SUP], Nariman R Battulin[SUP] 3 5 [/SUP], Alexander M Shestopalov[SUP] 4 5 [/SUP], Alexander V Taranin[SUP] 1 [/SUP]
Affiliations
- PMID: 39100673
- PMCID: PMC11294225
- DOI: 10.3389/fimmu.2024.1442160
The COVID-19 pandemic has uncovered the high genetic variability of the SARS-CoV-2 virus and its ability to evade the immune responses that were induced by earlier viral variants. Only a few monoclonal antibodies that have been reported to date are capable of neutralizing a broad spectrum of SARS-CoV-2 variants. Here, we report the isolation of a new broadly neutralizing human monoclonal antibody, iC1. The antibody was identified through sorting the SARS-CoV-1 RBD-stained individual B cells that were isolated from the blood of a vaccinated donor following a breakthrough infection. In vitro, iC1 potently neutralizes pseudoviruses expressing a wide range of SARS-CoV-2 Spike variants, including those of the XBB sublineage. In an hACE2-transgenic mouse model, iC1 provided effective protection against the Wuhan strain of the virus as well as the BA.5 and XBB.1.5 variants. Therefore, iC1 can be considered as a potential component of the broadly neutralizing antibody cocktails resisting the SARS-CoV-2 mutation escape.
Keywords: COVID-19; Omicron; SARS-CoV-1; iC1 antibody; in vivo protection.