tetano
Editor, Senior Moderator
Front Cell Infect Microbiol
. 2022 Jan 20;11:827790.
doi: 10.3389/fcimb.2021.827790. eCollection 2021.
Generation of a pdmH1N1 2018 Influenza A Reporter Virus Carrying a mCherry Fluorescent Protein in the PA Segment
Ling Bu[SUP] 1 2 [/SUP], Boqian Chen[SUP] 3 [/SUP], Lei Xing[SUP] 1 2 [/SUP], Xuejun Cai[SUP] 1 2 [/SUP], Shuhua Liang[SUP] 1 2 [/SUP], Liying Zhang[SUP] 4 [/SUP], Xinhua Wang[SUP] 1 2 3 [/SUP], Wenjun Song[SUP] 1 2 5 [/SUP]
Affiliations
Abstract
Influenza A virus (IAV) is a major human pathogen associated with significant morbidity and mortality worldwide. Through serial passage in mice, we generated a recombinant pdmH1N1 2009 IAV, A/Guangdong/GLW/2018 (GLW/18-MA), which encodes an mCherry gene fused to the C-terminal of a polymerase acidic (PA) segment and demonstrated comparable growth kinetics to the wild-type. Nine mutations were identified in the GLW/18-MA genome: PA (I61M, E351G, and G631S), NP (E292G), HA1 (T164I), HA2 (N117S and P160S), NA (W61R), and NEP (K44R). The recombinant IAV reporter expresses mCherry, a red fluorescent protein, at a high level and maintains its genetic integrity after five generations of serial passages in Madin-Darby Canine Kidney cells (MDCK) cells. Moreover, the imaging is noninvasive and permits the monitoring of infection in living mice. Treatment with oseltamivir or baicalin followed by infection with the reporter IAV led to a decrease in fluorescent protein signal in living mice. This result demonstrates that the IAV reporter virus is a powerful tool to study viral pathogenicity and transmission and to develop and evaluate novel anti-viral drugs, inhibitors, and vaccines in the future.
Keywords: H1N1; fluorescent virus; influenza A virus; mouse adaptation; reporter virus.
. 2022 Jan 20;11:827790.
doi: 10.3389/fcimb.2021.827790. eCollection 2021.
Generation of a pdmH1N1 2018 Influenza A Reporter Virus Carrying a mCherry Fluorescent Protein in the PA Segment
Ling Bu[SUP] 1 2 [/SUP], Boqian Chen[SUP] 3 [/SUP], Lei Xing[SUP] 1 2 [/SUP], Xuejun Cai[SUP] 1 2 [/SUP], Shuhua Liang[SUP] 1 2 [/SUP], Liying Zhang[SUP] 4 [/SUP], Xinhua Wang[SUP] 1 2 3 [/SUP], Wenjun Song[SUP] 1 2 5 [/SUP]
Affiliations
- PMID: 35127568
- PMCID: PMC8811159
- DOI: 10.3389/fcimb.2021.827790
Abstract
Influenza A virus (IAV) is a major human pathogen associated with significant morbidity and mortality worldwide. Through serial passage in mice, we generated a recombinant pdmH1N1 2009 IAV, A/Guangdong/GLW/2018 (GLW/18-MA), which encodes an mCherry gene fused to the C-terminal of a polymerase acidic (PA) segment and demonstrated comparable growth kinetics to the wild-type. Nine mutations were identified in the GLW/18-MA genome: PA (I61M, E351G, and G631S), NP (E292G), HA1 (T164I), HA2 (N117S and P160S), NA (W61R), and NEP (K44R). The recombinant IAV reporter expresses mCherry, a red fluorescent protein, at a high level and maintains its genetic integrity after five generations of serial passages in Madin-Darby Canine Kidney cells (MDCK) cells. Moreover, the imaging is noninvasive and permits the monitoring of infection in living mice. Treatment with oseltamivir or baicalin followed by infection with the reporter IAV led to a decrease in fluorescent protein signal in living mice. This result demonstrates that the IAV reporter virus is a powerful tool to study viral pathogenicity and transmission and to develop and evaluate novel anti-viral drugs, inhibitors, and vaccines in the future.
Keywords: H1N1; fluorescent virus; influenza A virus; mouse adaptation; reporter virus.