tetano
Editor, Senior Moderator
Forensic Sci Int
. 2021 Dec 24;331:111168.
doi: 10.1016/j.forsciint.2021.111168. Online ahead of print.
SmartAmp method can rapidly detect SARS-CoV-2 in dead bodies
Sayaka Nagasawa[SUP] 1 [/SUP], Aika Mori[SUP] 2 [/SUP], Yuichiro Hirata[SUP] 2 [/SUP], Ayumi Motomura[SUP] 3 [/SUP], Namiko Ishii[SUP] 3 [/SUP], Keisuke Okaba[SUP] 4 [/SUP], Kie Horioka[SUP] 4 [/SUP], Yohsuke Makino[SUP] 5 [/SUP], Makoto Nakajima[SUP] 6 [/SUP], Suguru Torimitsu[SUP] 5 [/SUP], Rutsuko Yamaguchi[SUP] 5 [/SUP], Go Inokuchi[SUP] 5 [/SUP], Fumiko Chiba[SUP] 5 [/SUP], Yumi Hoshioka[SUP] 2 [/SUP], Naoki Saito[SUP] 2 [/SUP], Maiko Yoshida[SUP] 2 [/SUP], Daisuke Yajima[SUP] 3 [/SUP], Shinji Akitomi[SUP] 7 [/SUP], Hirotaro Iwase[SUP] 5 [/SUP], Hisako Saitoh[SUP] 2 [/SUP]
Affiliations
Abstract
Rapid and accurate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in dead bodies is essential to prevent infection among those working with dead bodies. This study focused on the Smart Amplification (SmartAmp) method, which has a short examination time (approximately an hour), is simple to perform, and demonstrates high specificity and sensitivity. This method has already been used for clinical specimens; however, its effectiveness in dead bodies has not been reported. This study examined the SmartAmp method using 11 autopsies or postmortem needle biopsies performed from January to May, 2021 (of these, five cases tested positive for SARS-CoV-2 by quantitative real-time polymerase chain reaction (qRT-PCR) and six cases tested negative). Swab samples were collected from the nasopharynx, oropharynx, or anus and the SmartAmp and qRT-PCR results were compared. For the nasopharynx and oropharynx samples, the same results were obtained for both methods in all cases; however, for the anal swabs, there was one case that was positive according to qRT-PCR but negative according to the SmartAmp method. The SmartAmp method may therefore be less sensitive than qRT-PCR and results may differ in specimens with a low viral load, such as anal swabs. However, in the nasopharynx and oropharynx specimens, which are normally used for testing, the results were the same using each method, suggesting that the SmartAmp method is useful in dead bodies. In the future, the SmartAmp method may be applied not only during autopsies, but also in various situations where dead bodies are handled.
Keywords: Autopsy; Rapid test; SARS-CoV-2; SmartAmp method; Swab test.
. 2021 Dec 24;331:111168.
doi: 10.1016/j.forsciint.2021.111168. Online ahead of print.
SmartAmp method can rapidly detect SARS-CoV-2 in dead bodies
Sayaka Nagasawa[SUP] 1 [/SUP], Aika Mori[SUP] 2 [/SUP], Yuichiro Hirata[SUP] 2 [/SUP], Ayumi Motomura[SUP] 3 [/SUP], Namiko Ishii[SUP] 3 [/SUP], Keisuke Okaba[SUP] 4 [/SUP], Kie Horioka[SUP] 4 [/SUP], Yohsuke Makino[SUP] 5 [/SUP], Makoto Nakajima[SUP] 6 [/SUP], Suguru Torimitsu[SUP] 5 [/SUP], Rutsuko Yamaguchi[SUP] 5 [/SUP], Go Inokuchi[SUP] 5 [/SUP], Fumiko Chiba[SUP] 5 [/SUP], Yumi Hoshioka[SUP] 2 [/SUP], Naoki Saito[SUP] 2 [/SUP], Maiko Yoshida[SUP] 2 [/SUP], Daisuke Yajima[SUP] 3 [/SUP], Shinji Akitomi[SUP] 7 [/SUP], Hirotaro Iwase[SUP] 5 [/SUP], Hisako Saitoh[SUP] 2 [/SUP]
Affiliations
- PMID: 34999365
- DOI: 10.1016/j.forsciint.2021.111168
Abstract
Rapid and accurate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in dead bodies is essential to prevent infection among those working with dead bodies. This study focused on the Smart Amplification (SmartAmp) method, which has a short examination time (approximately an hour), is simple to perform, and demonstrates high specificity and sensitivity. This method has already been used for clinical specimens; however, its effectiveness in dead bodies has not been reported. This study examined the SmartAmp method using 11 autopsies or postmortem needle biopsies performed from January to May, 2021 (of these, five cases tested positive for SARS-CoV-2 by quantitative real-time polymerase chain reaction (qRT-PCR) and six cases tested negative). Swab samples were collected from the nasopharynx, oropharynx, or anus and the SmartAmp and qRT-PCR results were compared. For the nasopharynx and oropharynx samples, the same results were obtained for both methods in all cases; however, for the anal swabs, there was one case that was positive according to qRT-PCR but negative according to the SmartAmp method. The SmartAmp method may therefore be less sensitive than qRT-PCR and results may differ in specimens with a low viral load, such as anal swabs. However, in the nasopharynx and oropharynx specimens, which are normally used for testing, the results were the same using each method, suggesting that the SmartAmp method is useful in dead bodies. In the future, the SmartAmp method may be applied not only during autopsies, but also in various situations where dead bodies are handled.
Keywords: Autopsy; Rapid test; SARS-CoV-2; SmartAmp method; Swab test.