tetano
Editor, Senior Moderator
ACS Appl Mater Interfaces. 2019 Oct 15. doi: 10.1021/acsami.9b16718. [Epub ahead of print] [h=1]Fluorescent-Magnetic-Catalytic Nanospheres for Dual-Modality Detection of H9N2 Avian Influenza Virus.[/h]
Peng X, Luo G, Wu Z, Wen W, Zhang X, Wang SF.
[h=3]Abstract[/h] The outbreak of H9N2 avian influenza virus (H9N2 AIV) brings high mortality and huge economic losses every year. Sensitive and reliable detection methods are essential to timely diagnosis and treatment. Herein, a dual-modality immunoassay is proposed for H9N2 AIV detection by employing fluorescent-magnetic-catalytic nanospheres (FMCNs) as labels and alkaline phosphatase (ALP)-induced metallization as signal amplification strategy. The excellent magnetic properties of FMCNs make the assay a potential application in complex samples. And the wonderful fluorescence properties of FMCNs enable fluorescence modality readout. The antibodies on FMCNs surface can achieve efficient capture and separation of targets. And amplified electrochemical modality readout can be obtained through ALP-catalyzed silver deposition. Dual-modality immunoassay combined the advantages of electrochemical assay with fluorescence assay provides accurate detection results to meet different testing needs. With two quantitative analysis forms, H9N2 AIV can be detected by electrochemical signals with a quantitation range of 0.1 to 1000 ng/mL and a detection limit of 10 pg/mL. The linear range is 300 to 1000 ng/mL with a detection limit of 69.8 ng/mL by fluorescence signal readout. Moreover, the specificity, anti-interference ability, accuracy and diversity of the proposal have unlimited potential for early diagnosis of suspect infections.
PMID: 31613583 DOI: 10.1021/acsami.9b16718
Peng X, Luo G, Wu Z, Wen W, Zhang X, Wang SF.
[h=3]Abstract[/h] The outbreak of H9N2 avian influenza virus (H9N2 AIV) brings high mortality and huge economic losses every year. Sensitive and reliable detection methods are essential to timely diagnosis and treatment. Herein, a dual-modality immunoassay is proposed for H9N2 AIV detection by employing fluorescent-magnetic-catalytic nanospheres (FMCNs) as labels and alkaline phosphatase (ALP)-induced metallization as signal amplification strategy. The excellent magnetic properties of FMCNs make the assay a potential application in complex samples. And the wonderful fluorescence properties of FMCNs enable fluorescence modality readout. The antibodies on FMCNs surface can achieve efficient capture and separation of targets. And amplified electrochemical modality readout can be obtained through ALP-catalyzed silver deposition. Dual-modality immunoassay combined the advantages of electrochemical assay with fluorescence assay provides accurate detection results to meet different testing needs. With two quantitative analysis forms, H9N2 AIV can be detected by electrochemical signals with a quantitation range of 0.1 to 1000 ng/mL and a detection limit of 10 pg/mL. The linear range is 300 to 1000 ng/mL with a detection limit of 69.8 ng/mL by fluorescence signal readout. Moreover, the specificity, anti-interference ability, accuracy and diversity of the proposal have unlimited potential for early diagnosis of suspect infections.
PMID: 31613583 DOI: 10.1021/acsami.9b16718