Mary Wilson
Well-known member
May 24/May 23 2022
Markus H. Antwerpen, Daniel Lang, Sabine Zange, Mathias C. Walter* and Roman Wölfel
Bundeswehr Institute of Microbiology, Munich, Germany
Since early May 2022, dozens of suspected and confirmed monkeypox infections have been reported in several European and North American countries. The first German monkeypox case was reported in Munich on May 19, where the 26 year-old patient had shown characteristic skin changes. During his travel through Europe, he finally showed mild symptoms and requested medical examination.
The Bundeswehr Institute of Microbiology performed primary diagnostics of a swab taken from a skin lesion and subsequently sequenced the sample. Here, we announce the available data to the scientific community.
DNA extraction was performed using DNeasy Mini Kit (Qiagen, Hilden, Germany) from clinical material and eluted in 100µl EB-Buffer. DNA concentrations were quantified using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Dreieich, Germany) according to the manufacturers’ protocols. From total DNA, an Illumina-compatible library was prepared (NEBNext® Ultra™ II DNA Library Prep Kit, NEB, Frankfurt am Main, Germany) and sequenced on a MiSeq instrument (Illumina, San Diego, CA, USA) using 2x 150bp v2 chemistry in order to obtained paired-end reads. ...
https://virological.org/t/first-ger...multi-country-outbreak-in-may-2022/812#post_1
Markus H. Antwerpen, Daniel Lang, Sabine Zange, Mathias C. Walter* and Roman Wölfel
Bundeswehr Institute of Microbiology, Munich, Germany
Since early May 2022, dozens of suspected and confirmed monkeypox infections have been reported in several European and North American countries. The first German monkeypox case was reported in Munich on May 19, where the 26 year-old patient had shown characteristic skin changes. During his travel through Europe, he finally showed mild symptoms and requested medical examination.
The Bundeswehr Institute of Microbiology performed primary diagnostics of a swab taken from a skin lesion and subsequently sequenced the sample. Here, we announce the available data to the scientific community.
DNA extraction was performed using DNeasy Mini Kit (Qiagen, Hilden, Germany) from clinical material and eluted in 100µl EB-Buffer. DNA concentrations were quantified using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Dreieich, Germany) according to the manufacturers’ protocols. From total DNA, an Illumina-compatible library was prepared (NEBNext® Ultra™ II DNA Library Prep Kit, NEB, Frankfurt am Main, Germany) and sequenced on a MiSeq instrument (Illumina, San Diego, CA, USA) using 2x 150bp v2 chemistry in order to obtained paired-end reads. ...
https://virological.org/t/first-ger...multi-country-outbreak-in-may-2022/812#post_1
