tetano
Editor, Senior Moderator
FEBS Open Bio
. 2022 Oct 22.
doi: 10.1002/2211-5463.13500. Online ahead of print.
Transcriptional dynamics of granulocytes in direct response to incubation with SARS-CoV-2
Daigo Nakazawa[SUP] #[/SUP][SUP] 1 [/SUP], Yohei Takeda[SUP] #[/SUP][SUP] 2 3 [/SUP], Masatoshi Kanda[SUP] #[/SUP][SUP] 4 [/SUP], Utano Tomaru[SUP] 5 [/SUP], Haruko Ogawa[SUP] 3 [/SUP], Takashi Kudo[SUP] 1 [/SUP], Satoka Shiratori-Aso[SUP] 1 [/SUP], Kanako Watanabe-Kusunoki[SUP] 1 [/SUP], Yusho Ueda[SUP] 1 [/SUP], Atsuko Miyoshi[SUP] 1 [/SUP], Fumihiko Hattanda[SUP] 1 [/SUP], Saori Nishio[SUP] 1 [/SUP], Ryo Uozumi[SUP] 6 [/SUP], Akihiro Ishizu[SUP] 7 [/SUP], Tatsuya Atsumi[SUP] 1 [/SUP]
Affiliations
Abstract
Severe coronavirus disease 2019 (COVID-19) is characterized by acute respiratory distress syndrome and multiple organ dysfunction, in which the host immune response plays a pivotal role. Excessive neutrophil activation and subsequent superfluity of neutrophil extracellular traps (NETs) can lead to tissue damage, and several studies have shown the involvement of neutrophils in severe COVID-19. However, the detailed responses of each neutrophil subset to SARS-CoV-2 infection has not been fully described. To explore this issue, we incubated normal-density granulocytes (NDGs) and low-density granulocytes (LDGs) with different viral titers of SARS-CoV-2. NDGs form NETs with chromatin fibers in response to SARS-CoV-2, whereas LDGs incubated with SARS-CoV-2 display a distinct morphology with condensed nuclei and moderate transcriptional changes. Based on these transcriptional changes, we suggest that AGO2 possibly plays a role in LDG regulation in response to SARS-CoV-2.
Keywords: SARS-CoV-2; granulocytes; low density granulocytes; neutrophil extracellular traps; severe COVID-19.
. 2022 Oct 22.
doi: 10.1002/2211-5463.13500. Online ahead of print.
Transcriptional dynamics of granulocytes in direct response to incubation with SARS-CoV-2
Daigo Nakazawa[SUP] #[/SUP][SUP] 1 [/SUP], Yohei Takeda[SUP] #[/SUP][SUP] 2 3 [/SUP], Masatoshi Kanda[SUP] #[/SUP][SUP] 4 [/SUP], Utano Tomaru[SUP] 5 [/SUP], Haruko Ogawa[SUP] 3 [/SUP], Takashi Kudo[SUP] 1 [/SUP], Satoka Shiratori-Aso[SUP] 1 [/SUP], Kanako Watanabe-Kusunoki[SUP] 1 [/SUP], Yusho Ueda[SUP] 1 [/SUP], Atsuko Miyoshi[SUP] 1 [/SUP], Fumihiko Hattanda[SUP] 1 [/SUP], Saori Nishio[SUP] 1 [/SUP], Ryo Uozumi[SUP] 6 [/SUP], Akihiro Ishizu[SUP] 7 [/SUP], Tatsuya Atsumi[SUP] 1 [/SUP]
Affiliations
- PMID: 36271697
- DOI: 10.1002/2211-5463.13500
Abstract
Severe coronavirus disease 2019 (COVID-19) is characterized by acute respiratory distress syndrome and multiple organ dysfunction, in which the host immune response plays a pivotal role. Excessive neutrophil activation and subsequent superfluity of neutrophil extracellular traps (NETs) can lead to tissue damage, and several studies have shown the involvement of neutrophils in severe COVID-19. However, the detailed responses of each neutrophil subset to SARS-CoV-2 infection has not been fully described. To explore this issue, we incubated normal-density granulocytes (NDGs) and low-density granulocytes (LDGs) with different viral titers of SARS-CoV-2. NDGs form NETs with chromatin fibers in response to SARS-CoV-2, whereas LDGs incubated with SARS-CoV-2 display a distinct morphology with condensed nuclei and moderate transcriptional changes. Based on these transcriptional changes, we suggest that AGO2 possibly plays a role in LDG regulation in response to SARS-CoV-2.
Keywords: SARS-CoV-2; granulocytes; low density granulocytes; neutrophil extracellular traps; severe COVID-19.