tetano
Editor, Senior Moderator
J Med Microbiol. 2011 Nov 17. [Epub ahead of print]
False positive PCR results linked to administration of seasonal influenza vaccine.
Curran T, McCaughey C, Ellis J, Mitchell SJ, Feeney SA, Watt AP, Mitchell F, Fairley DJ, Crawford L, McKenna J, Coyle PV.
Source
1 Regional Virus Laboratory, Dept of Microbiology, Belfast BT12 6BA;
Abstract
False positive PCRs occur as a consequence of specimen to specimen or amplicon to specimen contamination within the laboratory. Evidence of a novel contamination at time of specimen collection linked to influenza vaccine administration in the same location as influenza sampling is described. Clinical, circumstantial and laboratory evidence was gathered for each of 5 cases of influenza like illness (ILI) with unusual PCR patterns of positivity for seasonal H1N1, H3N2, H1N1 (2009), and influenza B viruses. Two trivalent influenza vaccine and environmental swabs of a hospital influenza vaccination room were also tested. Sequencing of influenza A matrix gene amplicons from the 5 cases and vaccines was undertaken. Four 2009 GP specimens were seasonal H1N1, H3N2 and influenza B PCR positive. One 2010 GP specimen was H1N1 (2009), H3N2 and influenza B positive. PCR of 2010 trivalent vaccines showed high loads of detectable influenza A and B RNA. Sequencing of the 5 specimens and vaccines showed greatest homology with M gene sequence of Influenza A/Puerto Rico/8/1934 H1N1 virus (used in generation of influenza vaccine strains). Environmental swabs had detectable influenza A and B RNA. Survival studies demonstrated vaccine RNA still detectable for at least 66 days. Administration of influenza vaccines in the same area as clinical sampling resulted in the contamination with vaccine strains of surveillance swabs collected from patients with ILI. Vaccine contamination should therefore be considered, particularly where multiple influenza virus RNA PCR positive signals (e.g. H1N1, H3N2 and influenza B) are detected in the same specimen.
PMID:
22096134
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22096134
False positive PCR results linked to administration of seasonal influenza vaccine.
Curran T, McCaughey C, Ellis J, Mitchell SJ, Feeney SA, Watt AP, Mitchell F, Fairley DJ, Crawford L, McKenna J, Coyle PV.
Source
1 Regional Virus Laboratory, Dept of Microbiology, Belfast BT12 6BA;
Abstract
False positive PCRs occur as a consequence of specimen to specimen or amplicon to specimen contamination within the laboratory. Evidence of a novel contamination at time of specimen collection linked to influenza vaccine administration in the same location as influenza sampling is described. Clinical, circumstantial and laboratory evidence was gathered for each of 5 cases of influenza like illness (ILI) with unusual PCR patterns of positivity for seasonal H1N1, H3N2, H1N1 (2009), and influenza B viruses. Two trivalent influenza vaccine and environmental swabs of a hospital influenza vaccination room were also tested. Sequencing of influenza A matrix gene amplicons from the 5 cases and vaccines was undertaken. Four 2009 GP specimens were seasonal H1N1, H3N2 and influenza B PCR positive. One 2010 GP specimen was H1N1 (2009), H3N2 and influenza B positive. PCR of 2010 trivalent vaccines showed high loads of detectable influenza A and B RNA. Sequencing of the 5 specimens and vaccines showed greatest homology with M gene sequence of Influenza A/Puerto Rico/8/1934 H1N1 virus (used in generation of influenza vaccine strains). Environmental swabs had detectable influenza A and B RNA. Survival studies demonstrated vaccine RNA still detectable for at least 66 days. Administration of influenza vaccines in the same area as clinical sampling resulted in the contamination with vaccine strains of surveillance swabs collected from patients with ILI. Vaccine contamination should therefore be considered, particularly where multiple influenza virus RNA PCR positive signals (e.g. H1N1, H3N2 and influenza B) are detected in the same specimen.
PMID:
22096134
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22096134