tetano
Editor, Senior Moderator
J Chromatogr A. 2019 Sep 27:460578. doi: 10.1016/j.chroma.2019.460578. [Epub ahead of print] [h=1]Fabrication of macroporous microspheres with core-shell structure for negative chromatography purification of virus.[/h]
An N[SUP]1[/SUP], Gong P[SUP]1[/SUP], Hou H[SUP]1[/SUP], Chi W[SUP]1[/SUP], Jin H[SUP]1[/SUP], Zhao L[SUP]2[/SUP], Tan Q[SUP]1[/SUP], Tang X[SUP]1[/SUP], Wang F[SUP]1[/SUP], Jin H[SUP]1[/SUP], Zhang R[SUP]3[/SUP].
[h=3]Author information[/h] 1 Beijing Key Laboratory of Fuels Cleaning and Advanced Catalytic Emission Reduction Technology/College of Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102617, China. 2 National Key Lab of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China. 3 Beijing Key Laboratory of Fuels Cleaning and Advanced Catalytic Emission Reduction Technology/College of Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102617, China. Electronic address: zhangrongyue@bipt.edu.cn.
[h=3]Abstract[/h] The macroporous microspheres with core-shell structure, based on a copolymer of 4-Vinylbenzyl chloride, glycidyl methacrylate, and ethylene glycol dimethacrylate, were fabricated through atom transfer radical polymerization suspension polymerization. The microspheres showed 100-200 nm pores in shell and 500-900 nm pores in core. The shell was hydrophilic modified through grafting of poly(N-hydroxyethyl acrylamide) onto the shell surface for reducing adsorption of proteins. The core was coupled with a ligand of poly(ethylene imine) that could bind the proteins. Feedstock of avian influenza virus could be purified on these modified microspheres through negative chromatography. Avian influenza virus cannot enter the core and was recovered from the flow-through, while other proteins with negative charges were able to penetrate into the core and bind to the poly(ethylene imine) ligands. The dynamic binding capacity of proteins was higher on this medium (61 mg/mL) than the commercially available resin (12 mg/mL, Capto Core 700).
Copyright ? 2019 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] Avian influenza virus; Macroporous microsphere with core-shell structure; Negative chromatography; Purification of virus
PMID: 31623846 DOI: 10.1016/j.chroma.2019.460578
An N[SUP]1[/SUP], Gong P[SUP]1[/SUP], Hou H[SUP]1[/SUP], Chi W[SUP]1[/SUP], Jin H[SUP]1[/SUP], Zhao L[SUP]2[/SUP], Tan Q[SUP]1[/SUP], Tang X[SUP]1[/SUP], Wang F[SUP]1[/SUP], Jin H[SUP]1[/SUP], Zhang R[SUP]3[/SUP].
[h=3]Author information[/h] 1 Beijing Key Laboratory of Fuels Cleaning and Advanced Catalytic Emission Reduction Technology/College of Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102617, China. 2 National Key Lab of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China. 3 Beijing Key Laboratory of Fuels Cleaning and Advanced Catalytic Emission Reduction Technology/College of Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102617, China. Electronic address: zhangrongyue@bipt.edu.cn.
[h=3]Abstract[/h] The macroporous microspheres with core-shell structure, based on a copolymer of 4-Vinylbenzyl chloride, glycidyl methacrylate, and ethylene glycol dimethacrylate, were fabricated through atom transfer radical polymerization suspension polymerization. The microspheres showed 100-200 nm pores in shell and 500-900 nm pores in core. The shell was hydrophilic modified through grafting of poly(N-hydroxyethyl acrylamide) onto the shell surface for reducing adsorption of proteins. The core was coupled with a ligand of poly(ethylene imine) that could bind the proteins. Feedstock of avian influenza virus could be purified on these modified microspheres through negative chromatography. Avian influenza virus cannot enter the core and was recovered from the flow-through, while other proteins with negative charges were able to penetrate into the core and bind to the poly(ethylene imine) ligands. The dynamic binding capacity of proteins was higher on this medium (61 mg/mL) than the commercially available resin (12 mg/mL, Capto Core 700).
Copyright ? 2019 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] Avian influenza virus; Macroporous microsphere with core-shell structure; Negative chromatography; Purification of virus
PMID: 31623846 DOI: 10.1016/j.chroma.2019.460578