tetano
Editor, Senior Moderator
F1000Res
. 2023 Jun 15;11:403.
doi: 10.12688/f1000research.110305.2. eCollection 2022. Effect of elevated temperature on SARS-CoV-2 viability
Harapan Harapan[SUP] 1 2 3 [/SUP], Edison Johar[SUP] 4 [/SUP], Chairin Nisa Maroef[SUP] 4 [/SUP], Ida Yus Sriyani[SUP] 4 [/SUP], Muhammad Iqhrammullah[SUP] 5 [/SUP], Hendrix Indra Kusuma[SUP] 3 6 [/SUP], Maimun Syukri[SUP] 7 [/SUP], Razali Razali[SUP] 8 [/SUP], Hamdani Hamdani[SUP] 8 [/SUP], Rudi Kurniawan[SUP] 8 [/SUP], Irwansyah Irwansyah[SUP] 8 [/SUP], Sarwo Edhy Sofyan[SUP] 8 [/SUP], Khin Saw Myint[SUP] 4 [/SUP], T M Indra Mahlia[SUP] 9 [/SUP], Samsul Rizal[SUP] 8 [/SUP]
Affiliations
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a worldwide disruption of global health putting healthcare workers at high risk. To reduce the transmission of SARS-CoV-2, in particular during treating the patients, our team aims to develop an optimized isolation chamber. The present study was conducted to evaluate the role of temperature elevation against SARS-CoV-2 viability, where the information would be used to build the isolation chamber. 0.6 mL of the Indonesian isolate of SARS-CoV-2 strain 20201012747 (approximately 10 [SUP]13[/SUP] PFU/mL) was incubated for one hour with a variation of temperatures: 25, 30, 35, 40, 45, 50, 55, 60, and 65°C in digital block heater as well as at room temperature (21-23°C) before used to infect Vero E6 cells. The viability was determined using a plaque assay. Our data found a significant reduction of the viral viability from 10 [SUP]13[/SUP] PFU/mL to 10 [SUP]9[/SUP] PFU/mL after the room temperature was increase to 40°C. Further elevation revealed that 55°C and above resulted in the total elimination of the viral viability. Increasing the temperature 40°C to reduce the SARS-CoV-2 survival could create mild hyperthermia conditions in a patient which could act as a thermotherapy. In addition, according to our findings, thermal sterilization of the vacant isolation chamber could be conducted by increasing the temperature to 55°C. In conclusion, elevating the temperature of the isolation chamber could be one of the main variables for developing an optimized isolation chamber for COVID-19 patients.
Keywords: COVID-19; Isolation chamber; SARS-CoV-2; Temperature; Transmission.
. 2023 Jun 15;11:403.
doi: 10.12688/f1000research.110305.2. eCollection 2022. Effect of elevated temperature on SARS-CoV-2 viability
Harapan Harapan[SUP] 1 2 3 [/SUP], Edison Johar[SUP] 4 [/SUP], Chairin Nisa Maroef[SUP] 4 [/SUP], Ida Yus Sriyani[SUP] 4 [/SUP], Muhammad Iqhrammullah[SUP] 5 [/SUP], Hendrix Indra Kusuma[SUP] 3 6 [/SUP], Maimun Syukri[SUP] 7 [/SUP], Razali Razali[SUP] 8 [/SUP], Hamdani Hamdani[SUP] 8 [/SUP], Rudi Kurniawan[SUP] 8 [/SUP], Irwansyah Irwansyah[SUP] 8 [/SUP], Sarwo Edhy Sofyan[SUP] 8 [/SUP], Khin Saw Myint[SUP] 4 [/SUP], T M Indra Mahlia[SUP] 9 [/SUP], Samsul Rizal[SUP] 8 [/SUP]
Affiliations
- PMID: 37745627
- PMCID: PMC10517306
- DOI: 10.12688/f1000research.110305.2
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a worldwide disruption of global health putting healthcare workers at high risk. To reduce the transmission of SARS-CoV-2, in particular during treating the patients, our team aims to develop an optimized isolation chamber. The present study was conducted to evaluate the role of temperature elevation against SARS-CoV-2 viability, where the information would be used to build the isolation chamber. 0.6 mL of the Indonesian isolate of SARS-CoV-2 strain 20201012747 (approximately 10 [SUP]13[/SUP] PFU/mL) was incubated for one hour with a variation of temperatures: 25, 30, 35, 40, 45, 50, 55, 60, and 65°C in digital block heater as well as at room temperature (21-23°C) before used to infect Vero E6 cells. The viability was determined using a plaque assay. Our data found a significant reduction of the viral viability from 10 [SUP]13[/SUP] PFU/mL to 10 [SUP]9[/SUP] PFU/mL after the room temperature was increase to 40°C. Further elevation revealed that 55°C and above resulted in the total elimination of the viral viability. Increasing the temperature 40°C to reduce the SARS-CoV-2 survival could create mild hyperthermia conditions in a patient which could act as a thermotherapy. In addition, according to our findings, thermal sterilization of the vacant isolation chamber could be conducted by increasing the temperature to 55°C. In conclusion, elevating the temperature of the isolation chamber could be one of the main variables for developing an optimized isolation chamber for COVID-19 patients.
Keywords: COVID-19; Isolation chamber; SARS-CoV-2; Temperature; Transmission.