tetano
Editor, Senior Moderator
Microbiol Immunol. 2012 Nov 6. doi: 10.1111/j.1348-0421.2012.00517.x. [Epub ahead of print]
Expression of recombinant HA1 protein for specific detection of influenza A/H1N1/ 2009 antibodies in human serum.
Luo L, Nishi K, Macleod E, Sabara MI, Coleman BL, Gubbay JB, Li Y.
Source
National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3M4 Department of Microbiology, Mount Sinai Hospital, 600 University Avenue, Toronto Ontario Canada M5G 1?5 Ontario Agency for Health Promotion & Protection, 81 Resources Road, Toronto, Ontario, Canada M9P 3T1 National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2.
Abstract
The hemagglutinin genes (HA1 subunit) from human and animal 2009 pandemic H1N1 virus isolates were expressed with a baculovirus vector. The recombinant HA1 (rHA1) protein-based ELISA was evaluated for detection of specific influenza A(H1N1)pdm09 antibodies in vaccinated human serum samples. The results showed that the rHA1-ELISA consistently discerned between antibodies recognizing the seasonal influenza H1N1 and pdm09 viruses, showing a concordance of 94% as compared to the hemagglutination inhibition (HI) test. This study suggests the utility of rHA1-ELISA in serosurveillance.
? 2012 The Societies and Wiley Publishing Asia Pty Ltd.
PMID:
23126568
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23126568
Expression of recombinant HA1 protein for specific detection of influenza A/H1N1/ 2009 antibodies in human serum.
Luo L, Nishi K, Macleod E, Sabara MI, Coleman BL, Gubbay JB, Li Y.
Source
National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3M4 Department of Microbiology, Mount Sinai Hospital, 600 University Avenue, Toronto Ontario Canada M5G 1?5 Ontario Agency for Health Promotion & Protection, 81 Resources Road, Toronto, Ontario, Canada M9P 3T1 National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2.
Abstract
The hemagglutinin genes (HA1 subunit) from human and animal 2009 pandemic H1N1 virus isolates were expressed with a baculovirus vector. The recombinant HA1 (rHA1) protein-based ELISA was evaluated for detection of specific influenza A(H1N1)pdm09 antibodies in vaccinated human serum samples. The results showed that the rHA1-ELISA consistently discerned between antibodies recognizing the seasonal influenza H1N1 and pdm09 viruses, showing a concordance of 94% as compared to the hemagglutination inhibition (HI) test. This study suggests the utility of rHA1-ELISA in serosurveillance.
? 2012 The Societies and Wiley Publishing Asia Pty Ltd.
PMID:
23126568
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23126568