tetano
Editor, Senior Moderator
Mol Med Report. 2012 Jul 26. doi: 10.3892/mmr.2012.1005. [Epub ahead of print]
Expression of hBD-2 induced by 23-valent pneumococcal polysaccharide vaccine, Haemophilus influenzae type b vaccine and split influenza virus vaccine.
Shen Z, Lei H.
Source
Department of Intensive Care Unit, Eastern Hospital, Tongji University, Shanghai 200120, P.R. China.
Abstract
Human β-defensin-2 (hBD-2) is an antimicrobial peptide with high activity and broad spectrum activity. hBD-2 expression may be highly elevated by microorganisms and inflammation. We reported that the majority of common vaccines used, including 23-valent pneumococcal polysaccharide vaccine, Haemophilus influenzae type b vaccine and split influenza virus vaccine, could induce the expression of hBD-2 in epithelial cells. Among them, the 23-valent pneumococcal polysaccharide vaccine was effective at a lower concentration (0.5 ?g/ml), while Haemophilus influenzae type b vaccine and split influenza virus vaccine were effective at the concentration of 1 ?g/ml. However, bacteriostatic experiments revealed that the split influenza virus vaccine was capable of inducing the highest antimicrobial activity. The medium of the 23-valent pneumococcal polysaccharide vaccine treatment group had a higher antimicrobial activity than the medium of the Haemophilus influenzae type b vaccine treatment group. The transcriptional regulator of hBD-2, that is, the NF-κB subunit, had a high level of activity, while the normal epithelial cells showed barely detectable activity, indicating that these vaccines have potential for clinical application.
PMID:
22842707
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22842707
Expression of hBD-2 induced by 23-valent pneumococcal polysaccharide vaccine, Haemophilus influenzae type b vaccine and split influenza virus vaccine.
Shen Z, Lei H.
Source
Department of Intensive Care Unit, Eastern Hospital, Tongji University, Shanghai 200120, P.R. China.
Abstract
Human β-defensin-2 (hBD-2) is an antimicrobial peptide with high activity and broad spectrum activity. hBD-2 expression may be highly elevated by microorganisms and inflammation. We reported that the majority of common vaccines used, including 23-valent pneumococcal polysaccharide vaccine, Haemophilus influenzae type b vaccine and split influenza virus vaccine, could induce the expression of hBD-2 in epithelial cells. Among them, the 23-valent pneumococcal polysaccharide vaccine was effective at a lower concentration (0.5 ?g/ml), while Haemophilus influenzae type b vaccine and split influenza virus vaccine were effective at the concentration of 1 ?g/ml. However, bacteriostatic experiments revealed that the split influenza virus vaccine was capable of inducing the highest antimicrobial activity. The medium of the 23-valent pneumococcal polysaccharide vaccine treatment group had a higher antimicrobial activity than the medium of the Haemophilus influenzae type b vaccine treatment group. The transcriptional regulator of hBD-2, that is, the NF-κB subunit, had a high level of activity, while the normal epithelial cells showed barely detectable activity, indicating that these vaccines have potential for clinical application.
PMID:
22842707
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22842707