Shiloh
Editor, Senior Moderator
Source: http://7thspace.com/headlines/34355...r_microcell_mediated_chromosome_transfer.html
Exploitation of the interaction of measles virus fusogenic envelope proteins with the surface receptor CD46 on human cells for microcell-mediated chromosome transfer
Microcell-mediated chromosome transfer (MMCT) is a technique by which a chromosome(s) is moved from donor to recipient cells by microcell fusion. Polyethylene glycol (PEG) has conventionally been used as a fusogen, and has been very successful in various genetic studies.
However, PEG is not applicable for all types of recipient cells, because of its cell type-dependent toxicity. The cytotoxicity of PEG limits the yield of microcell hybrids to low level (10-6 to 10-5 per recipient cells).
To harness the full potential of MMCT, a less toxic and more efficient fusion protocol that can be easily manipulated needs to be developed...
...Conclusions: Ectopic expression of MV envelope proteins provides an efficient recipient cell-oriented MMCT protocol, facilitating extensive applications for studies of gene function and genetic corrections.
Author: Motonobu KatohYasuhiro KazukiKanako KazukiNaoyo KajitaniMasato TakiguchiYuji NakayamaTakafumi NakamuraMitsuo Oshimura
Credits/Source: BMC Biotechnology 2010, 10:37
Exploitation of the interaction of measles virus fusogenic envelope proteins with the surface receptor CD46 on human cells for microcell-mediated chromosome transfer
Microcell-mediated chromosome transfer (MMCT) is a technique by which a chromosome(s) is moved from donor to recipient cells by microcell fusion. Polyethylene glycol (PEG) has conventionally been used as a fusogen, and has been very successful in various genetic studies.
However, PEG is not applicable for all types of recipient cells, because of its cell type-dependent toxicity. The cytotoxicity of PEG limits the yield of microcell hybrids to low level (10-6 to 10-5 per recipient cells).
To harness the full potential of MMCT, a less toxic and more efficient fusion protocol that can be easily manipulated needs to be developed...
...Conclusions: Ectopic expression of MV envelope proteins provides an efficient recipient cell-oriented MMCT protocol, facilitating extensive applications for studies of gene function and genetic corrections.
Author: Motonobu KatohYasuhiro KazukiKanako KazukiNaoyo KajitaniMasato TakiguchiYuji NakayamaTakafumi NakamuraMitsuo Oshimura
Credits/Source: BMC Biotechnology 2010, 10:37