tetano
Editor, Senior Moderator
Exp Ther Med
. 2023 Jul 6;26(2):398.
doi: 10.3892/etm.2023.12097. eCollection 2023 Aug. Reverse transcription loop‑mediated isothermal amplification has a high performance in the detection of SARS‑CoV‑2 in saliva samples and nasal swabs from asymptomatic and symptomatic individuals
Rodolfo Reyes-Morales[SUP] 1 [/SUP], Patricia Segundo-Ibañez[SUP] 2 [/SUP], César Flores-de Los Ángeles[SUP] 3 [/SUP], David Vizcarra-Ramos[SUP] 2 [/SUP], Damián Iñaki Ibañez-Galeana[SUP] 2 [/SUP], Gabriela Salas-Cuevas[SUP] 4 [/SUP], Ángel Olvera-Serrano[SUP] 4 [/SUP], Nancy Bibiana Pérez-Silva[SUP] 3 [/SUP], Valeria Magali Rocha-Rocha[SUP] 5 [/SUP], Elie Girgis El-Kassi[SUP] 5 [/SUP], Jorge Escobedo-Straffon[SUP] 5 [/SUP], Laura Contreras-Mioni[SUP] 5 [/SUP], Marisol Rosas-Díaz[SUP] 6 [/SUP], Karla María Lopez-Martinez[SUP] 1 [/SUP], Carlos Eduardo Arias-Matus[SUP] 1 [/SUP], Elizabeth Bautista-Rodriguez[SUP] 1 [/SUP], Manuel Nolasco-Quiroga[SUP] 4 [/SUP]
Affiliations
The detection of coronavirus disease 2019 cases represents a significant challenge at the epidemiological level. Limitations exist in effectively detecting asymptomatic cases, achieving good follow-up in hospitals without the infrastructure for reverse transcription-quantitative PCR (RT-qPCR) or in difficult-to-access areas and developing methods with the need for less invasive sampling procedures. Therefore, the present study evaluated the performance of the direct reverse transcription loop-mediated isothermal amplification (RT-LAMP) test for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the saliva and nasal samples of asymptomatic individuals belonging to the university population. In addition, this test was also assessed for effectiveness in symptomatic individuals referred from a hospital with poor infrastructure in molecular biology and located outside the urban area. The RT-LAMP assay was compared with the results obtained from the RT-qPCR nasopharyngeal swab test, where the diagnosis was confirmed by lateral flow immunoassay test for rapid antigen detection. A total of 128 samples were analyzed, of which 43% were symptomatic positive individuals, 25% were asymptomatic positive individuals and 32% were SARS-CoV2-negative control individuals. Among positive individuals, no differences were found between the Cq values determined by RT-qPCR. A sensitivity of 96.5% and a specificity of 97.6% was reported for the detection of SARS-CoV-2 in symptomatic individuals by salivary and nasal RT-LAMP, as well as a sensitivity of 100% and a specificity of 97.6% for the detection of SARS-CoV-2 in asymptomatic individuals. These findings indicated that performance of the direct RT-LAMP test using saliva and nasal samples has high sensitivity and specificity, which in turn suggest that it is a viable and reliable alternative for use in epidemiological monitoring.
Keywords: asymptomatic; nasal swab; reverse transcription loop-mediated isothermal amplification; saliva; severe acute respiratory syndrome coronavirus 2; symptomatic.
. 2023 Jul 6;26(2):398.
doi: 10.3892/etm.2023.12097. eCollection 2023 Aug. Reverse transcription loop‑mediated isothermal amplification has a high performance in the detection of SARS‑CoV‑2 in saliva samples and nasal swabs from asymptomatic and symptomatic individuals
Rodolfo Reyes-Morales[SUP] 1 [/SUP], Patricia Segundo-Ibañez[SUP] 2 [/SUP], César Flores-de Los Ángeles[SUP] 3 [/SUP], David Vizcarra-Ramos[SUP] 2 [/SUP], Damián Iñaki Ibañez-Galeana[SUP] 2 [/SUP], Gabriela Salas-Cuevas[SUP] 4 [/SUP], Ángel Olvera-Serrano[SUP] 4 [/SUP], Nancy Bibiana Pérez-Silva[SUP] 3 [/SUP], Valeria Magali Rocha-Rocha[SUP] 5 [/SUP], Elie Girgis El-Kassi[SUP] 5 [/SUP], Jorge Escobedo-Straffon[SUP] 5 [/SUP], Laura Contreras-Mioni[SUP] 5 [/SUP], Marisol Rosas-Díaz[SUP] 6 [/SUP], Karla María Lopez-Martinez[SUP] 1 [/SUP], Carlos Eduardo Arias-Matus[SUP] 1 [/SUP], Elizabeth Bautista-Rodriguez[SUP] 1 [/SUP], Manuel Nolasco-Quiroga[SUP] 4 [/SUP]
Affiliations
- PMID: 37522063
- PMCID: PMC10375439
- DOI: 10.3892/etm.2023.12097
The detection of coronavirus disease 2019 cases represents a significant challenge at the epidemiological level. Limitations exist in effectively detecting asymptomatic cases, achieving good follow-up in hospitals without the infrastructure for reverse transcription-quantitative PCR (RT-qPCR) or in difficult-to-access areas and developing methods with the need for less invasive sampling procedures. Therefore, the present study evaluated the performance of the direct reverse transcription loop-mediated isothermal amplification (RT-LAMP) test for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the saliva and nasal samples of asymptomatic individuals belonging to the university population. In addition, this test was also assessed for effectiveness in symptomatic individuals referred from a hospital with poor infrastructure in molecular biology and located outside the urban area. The RT-LAMP assay was compared with the results obtained from the RT-qPCR nasopharyngeal swab test, where the diagnosis was confirmed by lateral flow immunoassay test for rapid antigen detection. A total of 128 samples were analyzed, of which 43% were symptomatic positive individuals, 25% were asymptomatic positive individuals and 32% were SARS-CoV2-negative control individuals. Among positive individuals, no differences were found between the Cq values determined by RT-qPCR. A sensitivity of 96.5% and a specificity of 97.6% was reported for the detection of SARS-CoV-2 in symptomatic individuals by salivary and nasal RT-LAMP, as well as a sensitivity of 100% and a specificity of 97.6% for the detection of SARS-CoV-2 in asymptomatic individuals. These findings indicated that performance of the direct RT-LAMP test using saliva and nasal samples has high sensitivity and specificity, which in turn suggest that it is a viable and reliable alternative for use in epidemiological monitoring.
Keywords: asymptomatic; nasal swab; reverse transcription loop-mediated isothermal amplification; saliva; severe acute respiratory syndrome coronavirus 2; symptomatic.