tetano
Editor, Senior Moderator
J Clin Virol. 2014 Jun 30. pii: S1386-6532(14)00253-4. doi: 10.1016/j.jcv.2014.06.020. [Epub ahead of print]
Evolution of an influenza pandemic in 13 countries from 5 continents monitored by protein microarray from neonatal screening bloodspots.
de Bruin E1, Loeber JG2, Meijer A2, Castillo GM3, Cepeda ML4, Torres-Sep?lveda MR5, Borrajo GJ6, Caggana M7, Giguere Y8, Meyer M9, Fukushi M10, Devi AR11, Khneisser I12, Vilarinho L13, von D?beln U14, Torresani T15, Mackenzie J16, Zutt I2, Schipper M2, Elvers LH2, Koopmans MP17.
Author information
Abstract
BACKGROUND:
Because of lack of worldwide standardization of influenza virus surveillance, comparison between countries of impact of a pandemic is challenging. For that, other approaches to allow internationally comparative serosurveys are welcome.
OBJECTIVES:
Here we explore the use of neonatal screening dried blood spots to monitor the trends of the 2009 influenza A (H1N1) pdm virus by the use of a protein microarray.
STUDY DESIGN:
We contacted colleagues from neonatal screening laboratories and asked for their willingness to participate in a study by testing anonymized neonatal screening bloodspots collected during the course of the pandemic. In total, 7749 dried blood spots from 13 countries in 5 continents where analyzed by using a protein microarray containing HA1 recombinant proteins derived from pandemic influenza A (H1N1) 2009 as well as seasonal influenza viruses.
RESULTS:
Results confirm the early start of the pandemic with extensive circulation in the US and Canada, when circulation of the new virus was limited in other parts of the world. The data collected from sites in Mexico suggested limited circulation of the virus during the early pandemic phase in this country. In contrast and to our surprise, an increase in seroprevalence early in 2009 was noted in the dataset from Argentina, suggestive of much more widespread circulation of the novel virus in this country than in Mexico.
CONCLUSIONS:
We conclude that this uniform serological testing of samples from a highly standardized screening system offers an interesting opportunity for monitoring population level attack rates of widespread diseases outbreaks and pandemics.
Copyright ? 2014 The Authors. Published by Elsevier B.V. All rights reserved.
KEYWORDS:
Dried bloodspots; Influenza virus; Neonatal screening; Pandemic; Protein microarray
PMID:
25017954
[PubMed - as supplied by publisher]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/25017954
Evolution of an influenza pandemic in 13 countries from 5 continents monitored by protein microarray from neonatal screening bloodspots.
de Bruin E1, Loeber JG2, Meijer A2, Castillo GM3, Cepeda ML4, Torres-Sep?lveda MR5, Borrajo GJ6, Caggana M7, Giguere Y8, Meyer M9, Fukushi M10, Devi AR11, Khneisser I12, Vilarinho L13, von D?beln U14, Torresani T15, Mackenzie J16, Zutt I2, Schipper M2, Elvers LH2, Koopmans MP17.
Author information
Abstract
BACKGROUND:
Because of lack of worldwide standardization of influenza virus surveillance, comparison between countries of impact of a pandemic is challenging. For that, other approaches to allow internationally comparative serosurveys are welcome.
OBJECTIVES:
Here we explore the use of neonatal screening dried blood spots to monitor the trends of the 2009 influenza A (H1N1) pdm virus by the use of a protein microarray.
STUDY DESIGN:
We contacted colleagues from neonatal screening laboratories and asked for their willingness to participate in a study by testing anonymized neonatal screening bloodspots collected during the course of the pandemic. In total, 7749 dried blood spots from 13 countries in 5 continents where analyzed by using a protein microarray containing HA1 recombinant proteins derived from pandemic influenza A (H1N1) 2009 as well as seasonal influenza viruses.
RESULTS:
Results confirm the early start of the pandemic with extensive circulation in the US and Canada, when circulation of the new virus was limited in other parts of the world. The data collected from sites in Mexico suggested limited circulation of the virus during the early pandemic phase in this country. In contrast and to our surprise, an increase in seroprevalence early in 2009 was noted in the dataset from Argentina, suggestive of much more widespread circulation of the novel virus in this country than in Mexico.
CONCLUSIONS:
We conclude that this uniform serological testing of samples from a highly standardized screening system offers an interesting opportunity for monitoring population level attack rates of widespread diseases outbreaks and pandemics.
Copyright ? 2014 The Authors. Published by Elsevier B.V. All rights reserved.
KEYWORDS:
Dried bloodspots; Influenza virus; Neonatal screening; Pandemic; Protein microarray
PMID:
25017954
[PubMed - as supplied by publisher]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/25017954