Sally Furniss
Well-known member
EVIDENCE OF HUMAN-EQUINE INTERSPECIES INFLUENZA TRANSMISSION
Dalva A. P. Mancini, Elenice M. S. Cunha, Maria do Carmo C. S. H. Lara, Rita M. Z. Mendon?a, Andrea L. F. Dias, Alessandra F. de Castro, Jos? R. Pinto, Ronaldo Z. Mendon?a.
ABSTRACT
High levels of antibodies against the human influenza A(H1N1) and A(H3N2) strains in horse sera have been reported. The aim of the present study was to survey the human influenza virus strains present in horses using two methodologies, viral isolation and serology.
Sera and swabs (oro-nasal) were collected from horses belonging to stud and racing stables farms situated in S?o Paulo and Rio de Janeiro States, Brazil.
The HI test demonstrated that 46 horse serum samples presented antibodies to A/Eq1/SP/56(H7N7), A/Eq2/SP/1/85(H3N8) and A/SP/ /91(H1N1), A/SP/2/95(H3N2) and type B/SP/1/91, B/SP/6/2000 antigens of influenza virus.
Passages of 23 oro-nasal samples collected from race-horses and breeding horses were performed on MDCK cells.
After three and five passages of the samples, it was observed that 9 race-horses and 10 breeding horses showed CPE typical of influenza on MDCK cells.
These virus isolates had a mean HA titer of 4.3 HAU/25μl and 16.0 HAU/25μl, respectively and four horse samples were characterized by the Rapid Influenza A-B Test (Gloria-Roche).
The HI test demonstrated that 78.26%, 76.08%, 80.43% and 93.47% of the samples reacted to A/Eq1(H7N7), A/Eq2(H3N8), A(H1N1) and to A(H3N2) respectively, and 93.47% to type B/90 and 75% to type B/2000.
These results demonstrated that horses are reservoirs of the circulating influenza viruses of both the equine-specific and the non-specific strains.
http://www.sbv.org.br/revistavirusr&r/volume11pdf/Artigo Mancini et al. (b).pdf
Dalva A. P. Mancini, Elenice M. S. Cunha, Maria do Carmo C. S. H. Lara, Rita M. Z. Mendon?a, Andrea L. F. Dias, Alessandra F. de Castro, Jos? R. Pinto, Ronaldo Z. Mendon?a.
ABSTRACT
High levels of antibodies against the human influenza A(H1N1) and A(H3N2) strains in horse sera have been reported. The aim of the present study was to survey the human influenza virus strains present in horses using two methodologies, viral isolation and serology.
Sera and swabs (oro-nasal) were collected from horses belonging to stud and racing stables farms situated in S?o Paulo and Rio de Janeiro States, Brazil.
The HI test demonstrated that 46 horse serum samples presented antibodies to A/Eq1/SP/56(H7N7), A/Eq2/SP/1/85(H3N8) and A/SP/ /91(H1N1), A/SP/2/95(H3N2) and type B/SP/1/91, B/SP/6/2000 antigens of influenza virus.
Passages of 23 oro-nasal samples collected from race-horses and breeding horses were performed on MDCK cells.
After three and five passages of the samples, it was observed that 9 race-horses and 10 breeding horses showed CPE typical of influenza on MDCK cells.
These virus isolates had a mean HA titer of 4.3 HAU/25μl and 16.0 HAU/25μl, respectively and four horse samples were characterized by the Rapid Influenza A-B Test (Gloria-Roche).
The HI test demonstrated that 78.26%, 76.08%, 80.43% and 93.47% of the samples reacted to A/Eq1(H7N7), A/Eq2(H3N8), A(H1N1) and to A(H3N2) respectively, and 93.47% to type B/90 and 75% to type B/2000.
These results demonstrated that horses are reservoirs of the circulating influenza viruses of both the equine-specific and the non-specific strains.
http://www.sbv.org.br/revistavirusr&r/volume11pdf/Artigo Mancini et al. (b).pdf
