tetano
Editor, Senior Moderator
Clin Infect Dis. 2018 Feb 1;66(4):533-540. doi: 10.1093/cid/cix823.
[h=1]Evidence for Cross-species Influenza A Virus Transmission Within Swine Farms, China: A One Health, Prospective Cohort Study.[/h] Ma MJ[SUP]1[/SUP], Wang GL[SUP]1[/SUP], Anderson BD[SUP]2[/SUP], Bi ZQ[SUP]3,[/SUP][SUP]4[/SUP], Lu B[SUP]5[/SUP], Wang XJ[SUP]3,[/SUP][SUP]4[/SUP], Wang CX[SUP]6[/SUP], Chen SH[SUP]5[/SUP], Qian YH[SUP]5[/SUP], Song SX[SUP]3,[/SUP][SUP]4[/SUP], Li M[SUP]6[/SUP], Lednicky JA[SUP]7[/SUP], Zhao T[SUP]1[/SUP], Wu MN[SUP]1[/SUP], Cao WC[SUP]1[/SUP], Gray GC[SUP]2,[/SUP][SUP]8,[/SUP][SUP]9[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]Background:[/h] Our understanding of influenza A virus transmission between humans and pigs is limited.
[h=4]Methods:[/h] Beginning in 2015, we used a One Health approach and serial sampling to prospectively study 299 swine workers and 100 controls, their 9000 pigs, and 6 pig farm environments in China for influenza A viruses (IAVs) using molecular, culture, and immunological techniques. Study participants were closely monitored for influenza-like illness (ILI) events.
[h=4]Results:[/h] Upon enrollment, swine workers had higher serum neutralizing antibody titers against swine H1N1 and higher nasal wash total immunoglobulin A (IgA) and specific IgA titers against swine H1N1 and H3N2 viruses. Over a period of 12 months, IAVs were detected by quantitative reverse-transcription polymerase chain reaction in 46 of 396 (11.6%) environmental swabs, 235 of 3300 (7.1%) pig oral secretion, 23 of 396 (5.8%) water, 20 of 396 (5.1%) aerosol, and 19 of 396 (4.8%) fecal-slurry specimens. Five of 32 (15.6%) participants with ILI events had nasopharyngeal swab specimens that were positive for IAV, and 17 (53.1%) demonstrated 4-fold rises in neutralization titers against a swine virus. Reassorted Eurasian avian-lineage H1N1, A(H1N1)pdm09-like, and swine-lineage H3N2 viruses were identified in pig farms. The A(H1N1)pdm09-like H1N1 viruses identified in swine were nearly genetically identical to the human H1N1 viruses isolated from the participants with ILI.
[h=4]Conclusions:[/h] There was considerable evidence of A(H1N1)pdm09-like, swine-lineage H1N1, and swine-lineage H3N2 viruses circulating, likely reassorting, and likely crossing species within the pig farms. These data suggest that stronger surveillance for novel influenza virus emergence within swine farms is imperative.
[h=4]KEYWORDS:[/h] China; One Health; emerging pathogens; influenza A virus; swine
PMID: 29401271 DOI: 10.1093/cid/cix823
[h=1]Evidence for Cross-species Influenza A Virus Transmission Within Swine Farms, China: A One Health, Prospective Cohort Study.[/h] Ma MJ[SUP]1[/SUP], Wang GL[SUP]1[/SUP], Anderson BD[SUP]2[/SUP], Bi ZQ[SUP]3,[/SUP][SUP]4[/SUP], Lu B[SUP]5[/SUP], Wang XJ[SUP]3,[/SUP][SUP]4[/SUP], Wang CX[SUP]6[/SUP], Chen SH[SUP]5[/SUP], Qian YH[SUP]5[/SUP], Song SX[SUP]3,[/SUP][SUP]4[/SUP], Li M[SUP]6[/SUP], Lednicky JA[SUP]7[/SUP], Zhao T[SUP]1[/SUP], Wu MN[SUP]1[/SUP], Cao WC[SUP]1[/SUP], Gray GC[SUP]2,[/SUP][SUP]8,[/SUP][SUP]9[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]Background:[/h] Our understanding of influenza A virus transmission between humans and pigs is limited.
[h=4]Methods:[/h] Beginning in 2015, we used a One Health approach and serial sampling to prospectively study 299 swine workers and 100 controls, their 9000 pigs, and 6 pig farm environments in China for influenza A viruses (IAVs) using molecular, culture, and immunological techniques. Study participants were closely monitored for influenza-like illness (ILI) events.
[h=4]Results:[/h] Upon enrollment, swine workers had higher serum neutralizing antibody titers against swine H1N1 and higher nasal wash total immunoglobulin A (IgA) and specific IgA titers against swine H1N1 and H3N2 viruses. Over a period of 12 months, IAVs were detected by quantitative reverse-transcription polymerase chain reaction in 46 of 396 (11.6%) environmental swabs, 235 of 3300 (7.1%) pig oral secretion, 23 of 396 (5.8%) water, 20 of 396 (5.1%) aerosol, and 19 of 396 (4.8%) fecal-slurry specimens. Five of 32 (15.6%) participants with ILI events had nasopharyngeal swab specimens that were positive for IAV, and 17 (53.1%) demonstrated 4-fold rises in neutralization titers against a swine virus. Reassorted Eurasian avian-lineage H1N1, A(H1N1)pdm09-like, and swine-lineage H3N2 viruses were identified in pig farms. The A(H1N1)pdm09-like H1N1 viruses identified in swine were nearly genetically identical to the human H1N1 viruses isolated from the participants with ILI.
[h=4]Conclusions:[/h] There was considerable evidence of A(H1N1)pdm09-like, swine-lineage H1N1, and swine-lineage H3N2 viruses circulating, likely reassorting, and likely crossing species within the pig farms. These data suggest that stronger surveillance for novel influenza virus emergence within swine farms is imperative.
[h=4]KEYWORDS:[/h] China; One Health; emerging pathogens; influenza A virus; swine
PMID: 29401271 DOI: 10.1093/cid/cix823