tetano
Editor, Senior Moderator
Clin Vaccine Immunol. 2012 Jan 4. [Epub ahead of print]
Evaluation of three commercially available influenza A type-specific blocking-ELISA assays for seroepidemiological studies of influenza A virus infection in pigs.
Tse M, Kim M, Chan CH, Ho PL, Ma SK, Guan Y, Peiris JS.
Source
State Key Laboratory of Emerging Infectious Diseases & School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong Special Administrative Region, China.
Abstract
The reverse zoonotic transmission of the pandemic H1N1 2009 virus to swine necessitates enhanced surveillance of swine for influenza virus infection. Using a well characterized panel of naturally infected swine sera we evaluate and optimize the performance of three commercially available competitive ELISA assays, IDEXX? Influenza A Ab test, IDEXX? AI MultiS-Screen Ab Test and IDVet ID Screen? Influenza A Antibody Competition ELISA kit for detecting influenza type A reactive antibodies in swine. ROC analysis suggests that adjustment of the manufacturer recommended cut-off values would optimize sensitivity and specificity of these assays making them applicable for sero-epidemiology studies of swine influenza. Using such optimized cut-off levels, sensitivity and specificity of the IDEXX? Influenza A Ab test was 86% and 89% respectively; for IDEXX? AI MultiS-Screen Ab Test was 91% and 87% and for IDVet ID Screen? Influenza A was 95% and 79%, respectively.
PMID:
22219314
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22219314
Evaluation of three commercially available influenza A type-specific blocking-ELISA assays for seroepidemiological studies of influenza A virus infection in pigs.
Tse M, Kim M, Chan CH, Ho PL, Ma SK, Guan Y, Peiris JS.
Source
State Key Laboratory of Emerging Infectious Diseases & School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong Special Administrative Region, China.
Abstract
The reverse zoonotic transmission of the pandemic H1N1 2009 virus to swine necessitates enhanced surveillance of swine for influenza virus infection. Using a well characterized panel of naturally infected swine sera we evaluate and optimize the performance of three commercially available competitive ELISA assays, IDEXX? Influenza A Ab test, IDEXX? AI MultiS-Screen Ab Test and IDVet ID Screen? Influenza A Antibody Competition ELISA kit for detecting influenza type A reactive antibodies in swine. ROC analysis suggests that adjustment of the manufacturer recommended cut-off values would optimize sensitivity and specificity of these assays making them applicable for sero-epidemiology studies of swine influenza. Using such optimized cut-off levels, sensitivity and specificity of the IDEXX? Influenza A Ab test was 86% and 89% respectively; for IDEXX? AI MultiS-Screen Ab Test was 91% and 87% and for IDVet ID Screen? Influenza A was 95% and 79%, respectively.
PMID:
22219314
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22219314