tetano
Editor, Senior Moderator
PLoS One. 2017 Jan 30;12(1):e0170681. doi: 10.1371/journal.pone.0170681. eCollection 2017.
[h=1]Evaluation of the Protective Efficacy of Poly I:C as an Adjuvant for H9N2 Subtype Avian Influenza Inactivated Vaccine and Its Mechanism of Action in Ducks.[/h] Zhang A[SUP]1[/SUP], Lai H[SUP]2[/SUP], Xu J[SUP]2[/SUP], Huang W[SUP]2[/SUP], Liu Y[SUP]1[/SUP], Zhao D[SUP]1[/SUP], Chen R[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Current commercial H9 avian influenza vaccines cannot provide satisfactory protective immunity against antigenic variant influenza viruses in ducks. Poly I:C, when used as an adjuvant, improves humoral and cellular immunity in many animals but has not been tested in ducks. In this study, we investigated the protective efficacy of Poly I:C as an adjuvant for an inactivated H9N2 Avian influenza vaccine in ducks. We found that an H9N2 vaccine administered with poly I:C (H9-PIC vaccine) induced a significantly more rapid response with higher anti-influenza antibody titers than those of the vaccine alone (H9 vaccine). Moreover, virus shedding was reduced in ducks immunized with the H9-PIC vaccine after challenge with an H9 subtype antigenic variant viruses. IFN-α, IFN-γ, IL-6 and MHC-II mRNA levels were all elevated in ducks receiving the H9-PIC vaccine. In addition, lower expression level of MHC-I may be a reason for inefficient protective ability against heterologous influenza viruses in H9-PIC vaccination of ducks. In conclusion, poly I:C adjuvant enhanced both humoral and cellular immune responses in ducks induced by immunization of inactivated H9N2 vaccine.
PMID: 28135294 DOI: 10.1371/journal.pone.0170681
[PubMed - in process] Free full text
[h=1]Evaluation of the Protective Efficacy of Poly I:C as an Adjuvant for H9N2 Subtype Avian Influenza Inactivated Vaccine and Its Mechanism of Action in Ducks.[/h] Zhang A[SUP]1[/SUP], Lai H[SUP]2[/SUP], Xu J[SUP]2[/SUP], Huang W[SUP]2[/SUP], Liu Y[SUP]1[/SUP], Zhao D[SUP]1[/SUP], Chen R[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Current commercial H9 avian influenza vaccines cannot provide satisfactory protective immunity against antigenic variant influenza viruses in ducks. Poly I:C, when used as an adjuvant, improves humoral and cellular immunity in many animals but has not been tested in ducks. In this study, we investigated the protective efficacy of Poly I:C as an adjuvant for an inactivated H9N2 Avian influenza vaccine in ducks. We found that an H9N2 vaccine administered with poly I:C (H9-PIC vaccine) induced a significantly more rapid response with higher anti-influenza antibody titers than those of the vaccine alone (H9 vaccine). Moreover, virus shedding was reduced in ducks immunized with the H9-PIC vaccine after challenge with an H9 subtype antigenic variant viruses. IFN-α, IFN-γ, IL-6 and MHC-II mRNA levels were all elevated in ducks receiving the H9-PIC vaccine. In addition, lower expression level of MHC-I may be a reason for inefficient protective ability against heterologous influenza viruses in H9-PIC vaccination of ducks. In conclusion, poly I:C adjuvant enhanced both humoral and cellular immune responses in ducks induced by immunization of inactivated H9N2 vaccine.
PMID: 28135294 DOI: 10.1371/journal.pone.0170681
[PubMed - in process] Free full text