Mary Wilson
Well-known member
5 April 2023
DOI: https://doi.org/10.1128/jvi.00359-23
Authors: Maja Lang Balija https://orcid.org/0000-0003-2786-0054 mlbalija@unizg.hr, Adela Štimac, TanjaKošutić Gulija, Andrea Gudan Kurilj, Ana Bekavac, AntePlećaš, Beata Halassy, Maja Jagušić, Dubravko Forčić
ABSTRACT
Mumps is a highly contagious viral disease that can be prevented by vaccination. In the last decade, we have encountered repeated outbreaks of mumps in highly vaccinated populations, which call into question the effectiveness of available vaccines. Animal models are crucial for understanding virus-host interactions, and viruses such as mumps virus (MuV), whose only natural host is the human, pose a particular challenge. In our study, we examined the interaction between MuV and the guinea pig. Our results present the first evidence that guinea pigs of the Hartley strain can be infected in vivo after intranasal and intratesticular inoculation. We observed a significant viral replication in infected tissues up to 5 days following infection and induction of cellular and humoral immune responses as well as histopathological changes in infected lungs and testicles, without clinical signs of disease. Transmission of the infection through direct contact between animals was not possible. Our results demonstrate that guinea pigs and guinea pig primary cell cultures represent a promising model for immunological and pathogenetic studies of the complex MuV infection.
https://journals.asm.org/doi/10.1128/jvi.00359-23
DOI: https://doi.org/10.1128/jvi.00359-23
Authors: Maja Lang Balija https://orcid.org/0000-0003-2786-0054 mlbalija@unizg.hr, Adela Štimac, TanjaKošutić Gulija, Andrea Gudan Kurilj, Ana Bekavac, AntePlećaš, Beata Halassy, Maja Jagušić, Dubravko Forčić
ABSTRACT
Mumps is a highly contagious viral disease that can be prevented by vaccination. In the last decade, we have encountered repeated outbreaks of mumps in highly vaccinated populations, which call into question the effectiveness of available vaccines. Animal models are crucial for understanding virus-host interactions, and viruses such as mumps virus (MuV), whose only natural host is the human, pose a particular challenge. In our study, we examined the interaction between MuV and the guinea pig. Our results present the first evidence that guinea pigs of the Hartley strain can be infected in vivo after intranasal and intratesticular inoculation. We observed a significant viral replication in infected tissues up to 5 days following infection and induction of cellular and humoral immune responses as well as histopathological changes in infected lungs and testicles, without clinical signs of disease. Transmission of the infection through direct contact between animals was not possible. Our results demonstrate that guinea pigs and guinea pig primary cell cultures represent a promising model for immunological and pathogenetic studies of the complex MuV infection.
https://journals.asm.org/doi/10.1128/jvi.00359-23