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doi:10.1016/j.vaccine.2010.10.065 | How to Cite or Link Using DOI
Copyright ? 2010 Published by Elsevier Ltd.
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Evaluation of the cellular immune responses induced by a non-adjuvanted inactivated whole virus A/H5N1/VN/1203 pandemic influenza vaccine in humans
Brian A. Crowea, Corresponding Author Contact Information, E-mail The Corresponding Author, Peter Br?hla, Marijan Gerencera, Michael G. Schwendingera, Andreas Pilza, Otfried Kistnera, Katrin Koelling-Schlebuschb, Gerald Aichingerb, Julia Singerb, Markus Zeitlingerc, Markus M?llerc, Hartmut Ehrlichb and P. Noel Barretta
a Baxter Bioscience, Biomedical Research Centre, Uferstr. 15, A-2304 Orth an der Donau, Austria
b Baxter Bioscience, IZD Tower, Wagramerstr. 17-19, A-1220 Vienna, Austria
c Department of Clinical Pharmacology, Medical University, Vienna General Hospital, Waehringer Guertel 18-20, 1090 Vienna, Austria
Received 11 June 2010;
revised 21 October 2010;
accepted 24 October 2010.
Available online 4 November 2010.
Abstract
In the present study the homologous and heterologous type and subtype specific cellular immune response induced by a wild type inactivated whole virus H5N1 Influenza (A/Vietnam/1203/2004) vaccine was evaluated. Two immunizations with the Vero cell derived H5N1 influenza vaccine on Day 0 and Day 21 induced significant H5N1 vaccine specific and H5 haemagglutinin specific clade and cross-clade reactive CD4+ T cell responses, which were maintained at significant levels for at least 6 months. The H5N1 vaccine specific response cross-reacted with the H1N1, but not with H3N2 or B seasonal Influenza strains. The vaccine significantly increased the number of H5N1 specific and H5 haemagglutinin specific memory B cells, 6 months after the primary immunization, however no H1N1 specific cross-reactivity was observed. Importantly, the inactivated whole virus H5N1 vaccine was just as effective in inducing CD4+ T cell and memory B cell response in the elderly (60 years or over) as in the adult population (18?59 years).
http://www.sciencedirect.com/scienc...cee3c73ac1265f3055a33418473834db&searchtype=a
Copyright ? 2010 Published by Elsevier Ltd.
Permissions & Reprints
Evaluation of the cellular immune responses induced by a non-adjuvanted inactivated whole virus A/H5N1/VN/1203 pandemic influenza vaccine in humans
Brian A. Crowea, Corresponding Author Contact Information, E-mail The Corresponding Author, Peter Br?hla, Marijan Gerencera, Michael G. Schwendingera, Andreas Pilza, Otfried Kistnera, Katrin Koelling-Schlebuschb, Gerald Aichingerb, Julia Singerb, Markus Zeitlingerc, Markus M?llerc, Hartmut Ehrlichb and P. Noel Barretta
a Baxter Bioscience, Biomedical Research Centre, Uferstr. 15, A-2304 Orth an der Donau, Austria
b Baxter Bioscience, IZD Tower, Wagramerstr. 17-19, A-1220 Vienna, Austria
c Department of Clinical Pharmacology, Medical University, Vienna General Hospital, Waehringer Guertel 18-20, 1090 Vienna, Austria
Received 11 June 2010;
revised 21 October 2010;
accepted 24 October 2010.
Available online 4 November 2010.
Abstract
In the present study the homologous and heterologous type and subtype specific cellular immune response induced by a wild type inactivated whole virus H5N1 Influenza (A/Vietnam/1203/2004) vaccine was evaluated. Two immunizations with the Vero cell derived H5N1 influenza vaccine on Day 0 and Day 21 induced significant H5N1 vaccine specific and H5 haemagglutinin specific clade and cross-clade reactive CD4+ T cell responses, which were maintained at significant levels for at least 6 months. The H5N1 vaccine specific response cross-reacted with the H1N1, but not with H3N2 or B seasonal Influenza strains. The vaccine significantly increased the number of H5N1 specific and H5 haemagglutinin specific memory B cells, 6 months after the primary immunization, however no H1N1 specific cross-reactivity was observed. Importantly, the inactivated whole virus H5N1 vaccine was just as effective in inducing CD4+ T cell and memory B cell response in the elderly (60 years or over) as in the adult population (18?59 years).
http://www.sciencedirect.com/scienc...cee3c73ac1265f3055a33418473834db&searchtype=a