tetano
Editor, Senior Moderator
J Ethnopharmacol. 2011 Jun 12. [Epub ahead of print]
Evaluation of the anti-neuraminidase activity of the traditional Chinese medicines and determination of the anti-influenza A virus effects of the neuraminidase inhibitory TCMs in vitro and in vivo.
Tian L, Wang Z, Wu H, Wang S, Wang Y, Wang Y, Xu J, Wang L, Qi F, Fang M, Yu D, Fang X.
Source
Key Laboratory of Molecular Enzymology and Enzyme Engineering of Ministry Education, Jilin University, Changchun 130012, PR China.
Abstract
Influenza A viruses epidemic causes severe health problems in humans and animal species. Neuraminidase (NA) inhibitors are currently the most effective drugs to treat influenza A viruses infection. Here we tested the inhibitory activity of water extracts from 439 traditional Chinese medicines (TCMs) towards NA. Five TCMs, Duchesnea indica (Andr.) Focke [Fragaria indica Andr.], Liquidambar formosana Hance., Lithospermum erythrorhizon Sieb. et Zucc., Melia toosendan Sieb. et Zucc., and Prunella vulgaris L., exerted potent inhibitory activity towards NA. The in vitro anti-influenza virus activities of the 5 TCMs were evaluated using the strain A/California/7/2009(H1N1)NYMC X-179A of influenza A virus. The results showed that these TCMs in the range of 25-250μg/mL had the ability to reduce virus-induced cytopathic effect (CPE) and the virus yield in MDCK cells. The antiviral activities of Melia toosendan extract was further evaluated using a mouse model infected with influenza A virus, and the result showed it significantly reduced death rate and prolonged mean day to death (MDD) of the viral infected mice. In conclusion, our results showed that 5 TCMs exerted strong inhibitory activities towards NA, and exhibited antiviral effect against influenza A virus by reducing viral reproduction and reduced CPE of the viral infected cells. Furthermore, a randomly selected TCM with NA inhibitory activity, Melia toosendan, significantly reduced death rate and prolonged survival of the H1N1 viral infected mice.
Copyright ? 2011. Published by Elsevier Ireland Ltd.
PMID:
21699971
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21699971
Evaluation of the anti-neuraminidase activity of the traditional Chinese medicines and determination of the anti-influenza A virus effects of the neuraminidase inhibitory TCMs in vitro and in vivo.
Tian L, Wang Z, Wu H, Wang S, Wang Y, Wang Y, Xu J, Wang L, Qi F, Fang M, Yu D, Fang X.
Source
Key Laboratory of Molecular Enzymology and Enzyme Engineering of Ministry Education, Jilin University, Changchun 130012, PR China.
Abstract
Influenza A viruses epidemic causes severe health problems in humans and animal species. Neuraminidase (NA) inhibitors are currently the most effective drugs to treat influenza A viruses infection. Here we tested the inhibitory activity of water extracts from 439 traditional Chinese medicines (TCMs) towards NA. Five TCMs, Duchesnea indica (Andr.) Focke [Fragaria indica Andr.], Liquidambar formosana Hance., Lithospermum erythrorhizon Sieb. et Zucc., Melia toosendan Sieb. et Zucc., and Prunella vulgaris L., exerted potent inhibitory activity towards NA. The in vitro anti-influenza virus activities of the 5 TCMs were evaluated using the strain A/California/7/2009(H1N1)NYMC X-179A of influenza A virus. The results showed that these TCMs in the range of 25-250μg/mL had the ability to reduce virus-induced cytopathic effect (CPE) and the virus yield in MDCK cells. The antiviral activities of Melia toosendan extract was further evaluated using a mouse model infected with influenza A virus, and the result showed it significantly reduced death rate and prolonged mean day to death (MDD) of the viral infected mice. In conclusion, our results showed that 5 TCMs exerted strong inhibitory activities towards NA, and exhibited antiviral effect against influenza A virus by reducing viral reproduction and reduced CPE of the viral infected cells. Furthermore, a randomly selected TCM with NA inhibitory activity, Melia toosendan, significantly reduced death rate and prolonged survival of the H1N1 viral infected mice.
Copyright ? 2011. Published by Elsevier Ireland Ltd.
PMID:
21699971
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21699971