tetano
Editor, Senior Moderator
Diagn Microbiol Infect Dis. 2014 Dec 3. pii: S0732-8893(14)00484-2. doi: 10.1016/j.diagmicrobio.2014.11.013. [Epub ahead of print]
[h=1]Evaluation of novel second-generation RSV and influenza rapid tests at the point of care.[/h] Tuttle R[SUP]1[/SUP], Weick A[SUP]2[/SUP], Schwarz WS[SUP]3[/SUP], Chen X[SUP]4[/SUP], Obermeier P[SUP]5[/SUP], Seeber L[SUP]6[/SUP], Tief F[SUP]7[/SUP], Muehlhans S[SUP]8[/SUP], Karsch K[SUP]9[/SUP], Peiser C[SUP]10[/SUP], Duwe S[SUP]11[/SUP], Schweiger B[SUP]12[/SUP], Rath B[SUP]13[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Acute respiratory infections represent common pediatric emergencies. Infection control warrants immediate and accurate diagnoses. In the past, first-generation respiratory syncytial virus (RSV) rapid tests (strip tests) have shown suboptimal sensitivities. In 2013, the Food and Drug Administration licensed a second-generation RSV rapid test providing user-independent readouts (SOFIA?-RSV) using automated fluorescence assay technology known to yield superior results with influenza rapid testing. We are reporting the first point-of-care evaluation of the SOFIA?-RSV rapid test. In the Charit? Influenza-Like Disease Cohort, 686 nasopharyngeal samples were tested in parallel with SOFIA?-RSV and SOFIA?-Influenza A+B. Compared to real-time PCR, SOFIA?-RSV sensitivities/specificities were 78.6%/93.9%, respectively (SOFIA?-Influenza A: 80.6%/99.3%). Performance was greatest in patients below 2years of age with a test sensitivity of 81.8%. RSV sensitivities were highest (85%) in the first 2days of illness and with nasopharyngeal compared to nasal swabs (P=0.055, McNemar's test). Second-generation RSV and influenza rapid testing provides highly accurate results facilitating timely patient cohortation and management.
Copyright ? 2014 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Influenza-like illness; Minimally-invasive testing; Point-of-care diagnostics; Rapid testing; Respiratory syncytial virus
PMID: 25583129 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25583129
[h=1]Evaluation of novel second-generation RSV and influenza rapid tests at the point of care.[/h] Tuttle R[SUP]1[/SUP], Weick A[SUP]2[/SUP], Schwarz WS[SUP]3[/SUP], Chen X[SUP]4[/SUP], Obermeier P[SUP]5[/SUP], Seeber L[SUP]6[/SUP], Tief F[SUP]7[/SUP], Muehlhans S[SUP]8[/SUP], Karsch K[SUP]9[/SUP], Peiser C[SUP]10[/SUP], Duwe S[SUP]11[/SUP], Schweiger B[SUP]12[/SUP], Rath B[SUP]13[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Acute respiratory infections represent common pediatric emergencies. Infection control warrants immediate and accurate diagnoses. In the past, first-generation respiratory syncytial virus (RSV) rapid tests (strip tests) have shown suboptimal sensitivities. In 2013, the Food and Drug Administration licensed a second-generation RSV rapid test providing user-independent readouts (SOFIA?-RSV) using automated fluorescence assay technology known to yield superior results with influenza rapid testing. We are reporting the first point-of-care evaluation of the SOFIA?-RSV rapid test. In the Charit? Influenza-Like Disease Cohort, 686 nasopharyngeal samples were tested in parallel with SOFIA?-RSV and SOFIA?-Influenza A+B. Compared to real-time PCR, SOFIA?-RSV sensitivities/specificities were 78.6%/93.9%, respectively (SOFIA?-Influenza A: 80.6%/99.3%). Performance was greatest in patients below 2years of age with a test sensitivity of 81.8%. RSV sensitivities were highest (85%) in the first 2days of illness and with nasopharyngeal compared to nasal swabs (P=0.055, McNemar's test). Second-generation RSV and influenza rapid testing provides highly accurate results facilitating timely patient cohortation and management.
Copyright ? 2014 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Influenza-like illness; Minimally-invasive testing; Point-of-care diagnostics; Rapid testing; Respiratory syncytial virus
PMID: 25583129 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25583129