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Evaluation of new hemagglutinin-based rapid antigen test for influenza A pandemic (H1N1) 2009

tetano

Editor, Senior Moderator
J Clin Virol. 2010 Jul 19. [Epub ahead of print]
Evaluation of new hemagglutinin-based rapid antigen test for influenza A pandemic (H1N1) 2009.

Kim YK, Uh Y, Chun JK, Kim C, Kim HY.

Department of Internal Medicine, Yonsei University Wonju College of Medicine, Wonju, Republic of Korea.
Abstract

BACKGROUND: A new rapid antigen test (RAT), based on hemagglutinin, was developed for the improvement of influenza A pandemic (H1N1) 2009 detection. OBJECTIVE: To evaluate the performance of the new RAT for the diagnosis of influenza A pandemic (H1N1) 2009. STUDY DESIGN: The new RAT included 2009 H1N1 hemagglutinin-based band and influenza A and influenza B nucleoprotein-based bands. During the period from November 24, 2009 to December 14, 2009, 948 patients underwent the new RAT and real-time reverse transcriptase polymerase chain reaction (rRT-PCR) at the same time. The result of the new RAT was compared with that of rRT-PCR, and the results of hemagglutinin-based and nucleoprotein-based antigen tests were compared. RESULT: Among the 260 patients confirmed by rRT-PCR, 153 (58.8%) were positive in the nucleoprotein-based antigen test, and 182 (70.0%) were positive in the hemagglutinin-based antigen test. These results show that the new hemagglutinin-based antigen test was more sensitive than the nucleoprotein-based antigen test for the detection of influenza A pandemic (H1N1) 2009 (p<0.001, the McNemar test). CONCLUSION: The new hemagglutinin-based antigen test improved the sensitivity of diagnosis for influenza A pandemic (H1N1) 2009 and it might be helpful for the diagnosis of influenza A pandemic (H1N1) 2009. Copyright ? 2010 Elsevier B.V. All rights reserved.

PMID: 20663709 [PubMed - as supplied by publisher]

http://www.ncbi.nlm.nih.gov/pubmed/20663709
 
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