tetano
Editor, Senior Moderator
J Clin Virol. 2019 Mar 14;114:37-42. doi: 10.1016/j.jcv.2019.02.003. [Epub ahead of print]
[h=1]Evaluation of nasal levels of interferon and clinical severity of influenza in children.[/h] Comerlato Scotta M[SUP]1[/SUP], Greff Machado D[SUP]2[/SUP], Goecks Oliveira S[SUP]1[/SUP], de Moura A[SUP]1[/SUP], Rhoden Estorgato G[SUP]1[/SUP], de Souza APD[SUP]1[/SUP], Nery Porto B[SUP]1[/SUP], de Ara?jo PD[SUP]1[/SUP], Sarria EE[SUP]3[/SUP], Pitrez PM[SUP]4[/SUP], Jones MH[SUP]1[/SUP], Ara?jo Pinto L[SUP]1[/SUP], Tetelbom Stein R[SUP]1[/SUP], Polack FP[SUP]5[/SUP], Mattiello R[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Experimental data show that type I interferon has a key role in innate immune response against influenza infection.
[h=4]OBJECTIVE:[/h] We compared nasal levels of interferon-α2 and β among inpatients and outpatients with influenza.
[h=4]STUDY DESIGN:[/h] Children younger than 5 years of age with influenza-like illness seeking care at the emergency department within the first 72 h of disease onset were prospectively included. Clinical and demographic data and secretions through nasal wash were obtained. Influenza infection was assessed through reverse-transcription polymerase chain reaction and nasal levels of interferon-α2 and β were measured by enzyme-linked immunosorbent assay. All patients followed until the end of the disease.
[h=4]RESULTS:[/h] One hundred patients were included, of which 24 had confirmed influenza infection, and 5 of them were hospitalized. Subtypes A (H3N2) and B were confirmed in 10 and 14 patients, respectively. Seventy-six patients without influenza, including 48% of outpatients, were recruited as controls. All hospitalized patients were significantly younger regardless of influenza status (age <6 months in 59% vs. 23.2%, p < 0.001). All other data were similar among the groups. Comparing median levels of interferon-α2 among children with influenza, levels were significantly higher in outpatients than in hospitalized patients and were 263.2 pg/mL (25-75 interquartile range: 58.3-634) and detectable in only one patient (90 pg/mL), respectively. The levels of interferon-α2 in controls and those of interferon-β in all groups were not detected.
[h=4]CONCLUSIONS:[/h] Higher levels of interferon-α2 in patients- with less severe influenza reinforce experimental evidence about the protective role of interferon-α2 against influenza infection.
Copyright ? 2019 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] Child; Hospitalization; Human influenza; Innate immunity; Interferons
PMID: 30913521 DOI: 10.1016/j.jcv.2019.02.003
[h=1]Evaluation of nasal levels of interferon and clinical severity of influenza in children.[/h] Comerlato Scotta M[SUP]1[/SUP], Greff Machado D[SUP]2[/SUP], Goecks Oliveira S[SUP]1[/SUP], de Moura A[SUP]1[/SUP], Rhoden Estorgato G[SUP]1[/SUP], de Souza APD[SUP]1[/SUP], Nery Porto B[SUP]1[/SUP], de Ara?jo PD[SUP]1[/SUP], Sarria EE[SUP]3[/SUP], Pitrez PM[SUP]4[/SUP], Jones MH[SUP]1[/SUP], Ara?jo Pinto L[SUP]1[/SUP], Tetelbom Stein R[SUP]1[/SUP], Polack FP[SUP]5[/SUP], Mattiello R[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Experimental data show that type I interferon has a key role in innate immune response against influenza infection.
[h=4]OBJECTIVE:[/h] We compared nasal levels of interferon-α2 and β among inpatients and outpatients with influenza.
[h=4]STUDY DESIGN:[/h] Children younger than 5 years of age with influenza-like illness seeking care at the emergency department within the first 72 h of disease onset were prospectively included. Clinical and demographic data and secretions through nasal wash were obtained. Influenza infection was assessed through reverse-transcription polymerase chain reaction and nasal levels of interferon-α2 and β were measured by enzyme-linked immunosorbent assay. All patients followed until the end of the disease.
[h=4]RESULTS:[/h] One hundred patients were included, of which 24 had confirmed influenza infection, and 5 of them were hospitalized. Subtypes A (H3N2) and B were confirmed in 10 and 14 patients, respectively. Seventy-six patients without influenza, including 48% of outpatients, were recruited as controls. All hospitalized patients were significantly younger regardless of influenza status (age <6 months in 59% vs. 23.2%, p < 0.001). All other data were similar among the groups. Comparing median levels of interferon-α2 among children with influenza, levels were significantly higher in outpatients than in hospitalized patients and were 263.2 pg/mL (25-75 interquartile range: 58.3-634) and detectable in only one patient (90 pg/mL), respectively. The levels of interferon-α2 in controls and those of interferon-β in all groups were not detected.
[h=4]CONCLUSIONS:[/h] Higher levels of interferon-α2 in patients- with less severe influenza reinforce experimental evidence about the protective role of interferon-α2 against influenza infection.
Copyright ? 2019 Elsevier B.V. All rights reserved.
[h=4]KEYWORDS:[/h] Child; Hospitalization; Human influenza; Innate immunity; Interferons
PMID: 30913521 DOI: 10.1016/j.jcv.2019.02.003