tetano
Editor, Senior Moderator
Microbiol Immunol. 2019 May 9. doi: 10.1111/1348-0421.12687. [Epub ahead of print]
[h=1]Evaluation of influenza vaccine-induced cell-mediated immunity: Comparison between methods using peripheral blood mononuclear cells and whole blood.[/h] Otani N[SUP]1[/SUP], Shima M[SUP]1[/SUP], Ueda T[SUP]2[/SUP], Ichiki K[SUP]2[/SUP], Nakajima K[SUP]2[/SUP], Takesue Y[SUP]2[/SUP], Okuno T[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Assessments of cell-mediated immunity (CMI) might be critical to evaluate the ability of an individual to protect himself/herself against influenza virus infection. However, CMI has been difficult to evaluate because no simple CMI measurement method is available. We aimed to compare the performance of a CMI measurement method that we developed, which involves reacting whole blood with antigen, with the conventional method based on isolating peripheral blood mononuclear cells (PBMCs). We compared and tested correlations between these methods before and after vaccination in 26 healthy adults (28-58 years old; 12 men and 14 women). Changes in CMI after influenza vaccination in PBMCs cultured with antigen for 48 and 96 h and whole blood cultured with antigen for 48 h were studied. We found CMI measurement results using whole blood on day 2 and PBMCs on day 4 to be correlated. Spearman's correlation coefficients with four antigens [A (H1N1), A (H3N2), B (Yamagata lineage), and B (Victoria lineage)] before vaccination were 0.55, 0.61, 0.58, and 0.70, respectively, and were 0.40, 0.45, 0.62, and 0.52 after vaccination. CMI was detected sooner when whole blood was reacted with antigen than when PBMCs were reacted with antigen. The positive reaction rate of influenza A (H1N1 and H3N2) in whole blood on day 2 was higher than that in PBMCs on day 2. Our method is simple and may be useful for vaccine development as it can measure CMI with a small amount of blood without separation into PBMCs. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] cell-mediated immunity; influenza; peripheral blood mononuclear cell; vaccine; whole blood
PMID: 31070266 DOI: 10.1111/1348-0421.12687
[h=1]Evaluation of influenza vaccine-induced cell-mediated immunity: Comparison between methods using peripheral blood mononuclear cells and whole blood.[/h] Otani N[SUP]1[/SUP], Shima M[SUP]1[/SUP], Ueda T[SUP]2[/SUP], Ichiki K[SUP]2[/SUP], Nakajima K[SUP]2[/SUP], Takesue Y[SUP]2[/SUP], Okuno T[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Assessments of cell-mediated immunity (CMI) might be critical to evaluate the ability of an individual to protect himself/herself against influenza virus infection. However, CMI has been difficult to evaluate because no simple CMI measurement method is available. We aimed to compare the performance of a CMI measurement method that we developed, which involves reacting whole blood with antigen, with the conventional method based on isolating peripheral blood mononuclear cells (PBMCs). We compared and tested correlations between these methods before and after vaccination in 26 healthy adults (28-58 years old; 12 men and 14 women). Changes in CMI after influenza vaccination in PBMCs cultured with antigen for 48 and 96 h and whole blood cultured with antigen for 48 h were studied. We found CMI measurement results using whole blood on day 2 and PBMCs on day 4 to be correlated. Spearman's correlation coefficients with four antigens [A (H1N1), A (H3N2), B (Yamagata lineage), and B (Victoria lineage)] before vaccination were 0.55, 0.61, 0.58, and 0.70, respectively, and were 0.40, 0.45, 0.62, and 0.52 after vaccination. CMI was detected sooner when whole blood was reacted with antigen than when PBMCs were reacted with antigen. The positive reaction rate of influenza A (H1N1 and H3N2) in whole blood on day 2 was higher than that in PBMCs on day 2. Our method is simple and may be useful for vaccine development as it can measure CMI with a small amount of blood without separation into PBMCs. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] cell-mediated immunity; influenza; peripheral blood mononuclear cell; vaccine; whole blood
PMID: 31070266 DOI: 10.1111/1348-0421.12687